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<p class="MsoNormal">Dirk: do you know (or does anyone else) the effect of Phenoxyethanol on DNA? If it does not degrade it, then we should be aware that refixing in formalin will and that snips should thus be taken ahead of that stage if future gene work is
contemplated. <o:p></o:p></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black">Paul Callomon<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black">Collection Manager, Malacology and General Invertebrates<i><o:p></o:p></i></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:12.0pt;color:black">Academy of Natural Sciences of Drexel University</span></b><b><span style="font-size:12.0pt;color:black"><o:p></o:p></span></b></p>
<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black">1900 Benjamin Franklin Parkway, Philadelphia PA 19103-1195, USA<br>
<i><a href="mailto:prc44@drexel.edu"><span style="color:#0563C1">prc44@drexel.edu</span></a> Tel 215-405-5096 - Fax 215-299-1170</i><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-left:.5in"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu>
<b>On Behalf Of </b>Dirk Neumann<br>
<b>Sent:</b> Friday, November 12, 2021 2:53 AM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: [Nhcoll-l] Phenoxyethanol<o:p></o:p></p>
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<p><b><span style="font-family:"Arial",sans-serif;font-variant:small-caps">External.<o:p></o:p></span></b></p>
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<p class="MsoNormal" style="margin-left:.5in">Dear Tonya,<o:p></o:p></p>
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<p class="MsoNormal" style="margin-left:.5in">several recipes exist and have been used in collections since the mid 1950ies, mainly for marine invertebrates, but no only. Simon Moore
<a href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.natsca.org%2Fsites%2Fdefault%2Ffiles%2Fpublications%2Fbooks%2FFluid%2520Preservation.pdf&data=04%7C01%7Cprc44%40drexel.edu%7C939afca06c0f4ba50f7808d9a5b1b4fa%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C1%7C637723004955162859%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=qOGYqNfaSmkouCEmUgptIcSpMtYrAfsHwXyVltDMx%2F0%3D&reserved=0">
published</a> on it back in 1999 and colleagues from the NHM in London may chip-in.<o:p></o:p></p>
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<p class="MsoNormal" style="margin-left:.5in">John Simmons also has a own section on phenoxetol in his
<a href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Frowman.com%2FISBN%2F9781442229662%2FFluid-Preservation-A-Comprehensive-Reference&data=04%7C01%7Cprc44%40drexel.edu%7C939afca06c0f4ba50f7808d9a5b1b4fa%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C1%7C637723004955172856%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=HeuQSswtm30y5pq9UpmfuknnVezf9Z0gmg68WTRCIWo%3D&reserved=0">
Fluid Preservation book</a> (pages 69-70)<o:p></o:p></p>
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<p class="MsoNormal" style="margin-left:.5in">The recommendation we give in our forthcoming book is that specimens from phenoxetol should first be re-fixed in a 4% formaldehyde solution for few days (depending on specimen size, body mass etc.) and then stepped
up from 30-50-70% EtOH. This seems to have worked fine, but this experience so far refers exclusively to elasmobranchs, which tend to behave slightly different to fixatives and preservatives compared to, for example, bony fish (likely because of the cartilage).
Close monitoring during stepping specimens up surely is advised.<o:p></o:p></p>
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<p class="MsoNormal" style="margin-left:.5in">Hope this helps<o:p></o:p></p>
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<p class="MsoNormal" style="margin-left:.5in">Dirk<o:p></o:p></p>
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<p class="MsoNormal" style="margin-left:.5in">Am 12.11.2021 um 01:43 schrieb Haff, Tonya (NCMI, Crace):<o:p></o:p></p>
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<p class="MsoNormal" style="margin-left:.5in"><span style="font-size:12.0pt;color:black">Hello all,<o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-left:.5in"><span style="font-size:12.0pt;color:black">We just come upon some bird specimens in our collection that have been preserved with Phenoxyethanol. There are no notes on its concentration. The fluid needs to be changed
over. I think we should change it to 70% ETOH, but I am not sure if we should slowly step up the concentration, or if another solution would be preferable?<o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-left:.5in"><span style="font-size:12.0pt;color:black">Thanks!<o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-left:.5in"><span style="font-size:12.0pt;color:black">Tonya<o:p></o:p></span></p>
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<pre style="margin-left:.5in">_______________________________________________<o:p></o:p></pre>
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<pre style="margin-left:.5in">NHCOLL-L is brought to you by the Society for the Preservation of<o:p></o:p></pre>
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<pre style="margin-left:.5in">society. See <a href="https://nam10.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.spnhc.org%2F&data=04%7C01%7Cprc44%40drexel.edu%7C939afca06c0f4ba50f7808d9a5b1b4fa%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C1%7C637723004955192843%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=r82PWj4%2FNQlibn8aNSWByyAqJDSG7k%2FBazdRLH%2BZMWI%3D&reserved=0">http://www.spnhc.org</a> for membership information.<o:p></o:p></pre>
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<p class="MsoNormal" style="margin-left:.5in">-- <o:p></o:p></p>
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Dirk Neumann<br>
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