<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">Many thanks for these ‘aside’ observations Dave,<div class=""><br class=""></div><div class="">I know nothing of the DICE-CT technique having never needed to use it; however, the thought that Lugol’s iodine or the use of iodine as a pre-staining mordant for alizarin transparency preparations is most interesting. Do you or does anyone have any idea of how this enhancement might work?</div><div class=""><br class=""><div class=""><span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);">With all good wishes, Simon<br class=""><br class="">Simon Moore MIScT, RSci, FLS, ACR<br class="">Conservator of Natural Sciences and Cutlery Historian,</span><br class=""><br class=""><a href="http://www.natural-history-conservation.com" class="">www.natural-history-conservation.com</a><br class=""><br class=""><span></span><br class=""><span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none; caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0);"></span><span style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none;"></span><span><img apple-inline="yes" id="9C04849E-6A39-489C-978E-66D9BEF60DE5" src="cid:3543D570-FCD3-4F5F-B04C-2A6D3A8D9E27@home" class=""></span><span><img apple-inline="yes" id="29B72228-3BC8-4B55-B2A6-FEE9EC4F7851" src="cid:44ED9140-C81F-497F-B52B-A41A76C7F679@home" class=""></span><br class=""></div><br class=""><blockquote type="cite" class="">On 23 Mar 2022, at 18:49, David Cannatella <<a href="mailto:catfish@utexas.edu" class="">catfish@utexas.edu</a>> wrote:<br class=""><br class="">Hi all,<br class="">I thought I'd share some non-experimental observations on Lugol's solution and its effect on formalin fixed material, specifically frogs.<br class=""><br class="">I have not used in DICE-CT. However, I have dissected muscles on hundreds of frogs, using Lugol's to improve visualization of muscles and enhance photography.<br class=""><br class="">I used a "standard" histology-type solution of iodine and potassium iodide in 70% ethanol, not water. I'd reflect the skin to allow the stain to penetrate quickly, and did the dissections in layers, exposing deeper muscles and re-staining. I moved the specimen directly for storage alcohol to the Lugols with no rinsing. Often a specimen was re-stained several times, and destained in 70% ethanol (which was discarded after a few uses). I never noticed any desiccation or softening of the muscles or other organs such as the liver or intestines, but I was not collecting data on this.<br class="">I did not measure changes in pH, but I threw out the Lugols after a week to avoid potential acidification. I did not notice softening of bones, as some have seen. <br class=""><br class="">After muscle dissections, I would sometimes clear and stain the specimen using standard alizarin-alcian blue techniques. The specimens seemed to take up both the alizarin and alcian stains more quickly and evenly than did an untreated specimen. Perhaps this was a mordant effect of the iodine.<br class=""><br class="">Based on these qualitative but numerous observations, as a curator I would have little reservation about allowing DICE-CT staining of specimens, but bear in mind that I was working with generally smaller vertebrates that were sufficiently stained in 3-6 hours.<br class=""><br class="">Of course, it should be noted in the catalog that the specimen was treated in this way, to inform future researchers.<br class=""><br class="">Best,<br class="">Dave<br class=""><br class=""><br class=""><br class=""><br class=""><br class=""><br class=""><br class=""><br class="">On Wed, Mar 23, 2022 at 9:01 AM Bentley, Andrew Charles <<a href="mailto:abentley@ku.edu" class="">abentley@ku.edu</a>> wrote:<br class="">Esther<br class=""><br class=""> <br class=""><br class="">The oVert project here in the US has had extensive experience with diceCT and have developed the attached protocol. Jaimi Gray would be the person to contact in this regard - jaimigray@floridamuseum.ufl.edu. She also co-authored the following publication - https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467<br class=""><br class=""> <br class=""><br class="">Hope that helps<br class=""><br class=""> <br class=""><br class="">Andy<br class=""><br class=""> A : A : A :<br class=""> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br class=""> V V V<br class="">Andy Bentley<br class="">Ichthyology Collection Manager<br class="">University of Kansas<br class="">Biodiversity Institute<br class=""><br class="">Dyche Hall<br class="">1345 Jayhawk Boulevard<br class="">Lawrence, KS, 66045-7561<br class="">USA<br class=""><br class="">Tel: (785) 864-3863<br class="">Fax: (785) 864-5335 <br class="">Email: abentley@ku.edu <br class=""><br class="">ORCID: https://orcid.org/0000-0002-3093-1258<br class=""><br class="">http://ichthyology.biodiversity.ku.edu<br class=""><br class=""> A : A : A :<br class=""> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br class=""> V V V<br class=""><br class=""> <br class=""><br class="">From: Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> On Behalf Of Esther Dondorp<br class="">Sent: Wednesday, March 23, 2022 4:24 AM<br class="">To: nhcoll-l@mailman.yale.edu<br class="">Subject: [Nhcoll-l] DICE-CT on NHspecimens<br class=""><br class=""> <br class=""><br class="">Dear colleagues,<br class=""><br class=""> <br class=""><br class="">Does anyone have experience with Diffusible Iodine-based Contrast-enhanced Computed Tomography (DICE-CT) performed on fluid preserved natural history specimens? I want to know if it is really non destructive, if the specimens do stay stable for the next many years or more after this treatment? <br class=""><br class=""> <br class=""><br class="">Many thanks!<br class=""><br class=""> <br class=""><br class="">Esther Dondorp<br class=""><br class="">Senior Collectiebeheerder<br class=""><br class=""><br class=""><br class=""><br class=""><br class=""><br class=""><br class="">- -<br class="">esther.dondorp@naturalis.nl - www.naturalis.nl<br class="">Darwinweg 2, 2333 CR Leiden<br class="">Postbus 9517, 2300 RA Leiden<br class=""><br class=""><br class=""> <br class=""><br class="">_______________________________________________<br class="">Nhcoll-l mailing list<br class="">Nhcoll-l@mailman.yale.edu<br class="">https://mailman.yale.edu/mailman/listinfo/nhcoll-l<br class=""><br class="">_______________________________________________<br class="">NHCOLL-L is brought to you by the Society for the Preservation of<br class="">Natural History Collections (SPNHC), an international society whose<br class="">mission is to improve the preservation, conservation and management of<br class="">natural history collections to ensure their continuing value to<br class="">society. See http://www.spnhc.org for membership information.<br class="">Advertising on NH-COLL-L is inappropriate.<br class="">>> This message is from an external sender. Learn more about why this <<<br class="">>> matters at https://links.utexas.edu/rtyclf. <<<br class=""><br class=""><br class="">-- <br class="">David Cannatella <br class="">Assoc. Director, Biodiversity Center<br class="">Department of Integrative Biology<br class="">Patterson Labs, 2415 Speedway<br class="">University of Texas, Austin, Texas 78712<br class="">www.cannatellalab.org<br class="">512.453.1620 cell<br class="">_______________________________________________<br class="">Nhcoll-l mailing list<br class="">Nhcoll-l@mailman.yale.edu<br class="">https://mailman.yale.edu/mailman/listinfo/nhcoll-l<br class=""><br class="">_______________________________________________<br class="">NHCOLL-L is brought to you by the Society for the Preservation of<br class="">Natural History Collections (SPNHC), an international society whose<br class="">mission is to improve the preservation, conservation and management of<br class="">natural history collections to ensure their continuing value to<br class="">society. See http://www.spnhc.org for membership information.<br class="">Advertising on NH-COLL-L is inappropriate.<br class=""></blockquote><br class=""></div></body></html>