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<p class="MsoNormal">The biggest issue with diceCT has been soft-tissue shrinkage, but this has been remedied by using Sorensen’s buffer as published by Dawood et al. 2021. This is the buffer used in the Gray protocol shared by Andy. Links to the Dawood paper
as well as others of potential interest are below. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Dawood et al. 2021: <a href="https://www.nature.com/articles/s41598-021-99202-2">
https://www.nature.com/articles/s41598-021-99202-2</a><o:p></o:p></p>
<p class="MsoNormal">Early et al. 2020: <a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238783">
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238783</a><o:p></o:p></p>
<p class="MsoNormal">Hedrick et al. 2018: <a href="https://www.researchgate.net/publication/325862458_Assessing_Soft-Tissue_Shrinkage_Estimates_in_Museum_Specimens_Imaged_With_Diffusible_Iodine-Based_Contrast-Enhanced_Computed_Tomography_diceCT">
https://www.researchgate.net/publication/325862458_Assessing_Soft-Tissue_Shrinkage_Estimates_in_Museum_Specimens_Imaged_With_Diffusible_Iodine-Based_Contrast-Enhanced_Computed_Tomography_diceCT</a>
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Best,<o:p></o:p></p>
<p class="MsoNormal">Zach<o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">--</span> <o:p></o:p></p>
<p class="MsoNormal"><b><span style="color:#1F497D">Zachary S. Randall</span><o:p></o:p></b></p>
<p class="MsoNormal"><span style="color:#1F497D">Digital Imaging Manager </span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Florida Museum of Natural History
</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">1659 Museum Road, Gainesville, FL 32611-7800</span>
<o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">(352) 273-1958 | <a href="mailto:zrandall@flmnh.ufl.edu">
zrandall@flmnh.ufl.edu</a> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><a href="https://zachrandall.weebly.com/">https://zachrandall.weebly.com/</a><o:p></o:p></span></p>
<p class="MsoNormal"><a href="http://www.floridamuseum.ufl.edu/digital-lab/">www.floridamuseum.ufl.edu/digital-lab/</a><o:p></o:p></p>
<p class="MsoNormal"><a href="https://sketchfab.com/FloridaMuseum">https://sketchfab.com/FloridaMuseum</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> <b>
On Behalf Of </b>Bentley, Andrew Charles<br>
<b>Sent:</b> Wednesday, March 23, 2022 10:01 AM<br>
<b>To:</b> Esther Dondorp <esther.dondorp@naturalis.nl>; nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: [Nhcoll-l] DICE-CT on NHspecimens<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<b><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#FA4616">[External Email]</span></b>
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<p class="MsoNormal"><span style="color:black">Esther</span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">The oVert project here in the US has had extensive experience with diceCT and have developed the attached protocol. Jaimi Gray would be the person to contact in this regard - jaimigray@floridamuseum.ufl.edu. She also co-authored the following
publication - <a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__onlinelibrary.wiley.com_doi_full_10.1002_ece3.7467&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=PCoqPP0RplincQmDRfDfKbAf08gmtmu0zMnevmF7kYE&e=">
https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Hope that helps<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Andy<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU">Dyche Hall<br>
<a href="x-apple-data-detectors://9/"><span style="color:#0563C1">1345 Jayhawk Boulevard</span></a><br>
<a href="x-apple-data-detectors://9/"><span style="color:#0563C1">Lawrence, KS, 66045-7561</span></a><br>
<a href="x-apple-data-detectors://9/"><span style="color:#0563C1">USA</span></a><br>
<br>
Tel: <a href="tel:%28785%29%20864-3863" target="_blank"><span style="color:#0563C1">(785) 864-3863</span></a><br>
Fax: <a href="tel:%28785%29%20864-5335" target="_blank"><span style="color:#0563C1">(785) 864-5335</span></a> <br>
Email: <a href="mailto:abentley@ku.edu" target="_blank"><span style="color:#0563C1">abentley@ku.edu</span></a> <o:p></o:p></span></p>
<p class="MsoNormal">ORCID: <span style="color:#494A4C;background:white"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__orcid.org_0000-2D0002-2D3093-2D1258&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=W6K85Ze9PmgioL9GHoEqKgA6yf-ISXmJ6QyjpUy0Uyw&e="><span style="color:#0563C1">https://orcid.org/0000-0002-3093-1258</span></a><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu_&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=ANS6f0lN9_8S28FjQKr0jwy3geQCl1FB4n7T1ALKhwg&e=" target="_blank"><span style="color:#0563C1">http://ichthyology.biodiversity.ku.edu</span></a></span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> <b>
On Behalf Of </b>Esther Dondorp<br>
<b>Sent:</b> Wednesday, March 23, 2022 4:24 AM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [Nhcoll-l] DICE-CT on NHspecimens<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Dear colleagues,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Does anyone have experience with <span style="font-family:"Helvetica",sans-serif;color:#404040">Diffusible Iodine-based Contrast-enhanced Computed Tomography</span> (DICE-CT) performed on fluid preserved natural history specimens? I want
to know if it is really non destructive, if the specimens do stay stable for the next many years or more after this treatment? <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Many thanks!<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt">Esther Dondorp</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt">Senior Collectiebeheerder</span><o:p></o:p></p>
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<sub><span style="font-size:8.0pt">- -</span></sub><br>
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