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<p class="MsoNormal">I agree with everything Greg stated and we consider it destructive sampling as well. The staining and other alterations are permanent and specimens should be stored separately when returned to the collection with notes about the staining
and scanning methods used. In the birds we tested, the internal organs are stained permanently as shown in scans done before and after ‘destaining’.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Chris<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><b><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#1F497D">Christopher Milensky</span></b><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#1F497D">Collection Manager</span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#1F497D"><a href="https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Fvertebrates.si.edu%2Fbirds%2F&data=04%7C01%7CMilenskyC%40si.edu%7Cb95c5eae604e4f3c742408da0ce31373%7C989b5e2a14e44efe93b78cdd5fc5d11c%7C0%7C0%7C637836466677167556%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=LM0YKvSERd9QS1VHGqn2J58IBGIPTrpLf6ewI9Fb5Bk%3D&reserved=0" target="_blank">Division
of Birds</a></span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><b><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#1F497D">w</span></b><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#1F497D"> 202.633.0794
<a href="mailto:milenskyc@si.edu" target="_blank">milenskyc@si.edu</a></span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#00C1D5">SMITHSONIAN INSTITUTION</span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:9.0pt;font-family:"Arial",sans-serif;color:#00778B">NATIONAL MUSEUM OF NATURAL HISTORY</span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> <b>
On Behalf Of </b>Watkins-Colwell, Gregory<br>
<b>Sent:</b> Wednesday, March 23, 2022 9:59 AM<br>
<b>To:</b> Esther Dondorp <esther.dondorp@naturalis.nl>; nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> Re: [Nhcoll-l] DICE-CT on NHspecimens<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" style="line-height:12.0pt;background:#FFEB9C"><b><span style="font-size:9.0pt;color:#9C6500">External Email - Exercise Caution</span></b><o:p></o:p></p>
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<p class="MsoNormal">We consider it “destructive sampling” because the specimen is forever altered. Rinsing in multiple washes of ethanol can get the specimen back to near original color (though likely pulling out more pigments and other etoh-soluble organics
in the process). However, the odds are pretty good that there’s still iodine in the specimen somewhere… and that could impact future use. So because of that we consider it a form of destructive sampling.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I also suspect there is damage done during the long soak in the aqueous solution. I know some folks have used iodine dissolved in ethanol because of that reason and it may help, though it also might not stain as well thus permanently altering
a specimen for insufficient gain. I tend to just document the process, call it destructive, and not dictate that the iodine be in ETOH. I also request that it be returned in ETOH, but not “washed”. I can do that here and have more control over how many
ETOH soaks it gets before going back into the collection. Generally they all get their own jar too so they can’t accidentally stain a room-mate. And I’d rather there be slightly more iodine left in the specimen than risk damaging it by repeated ETOH changes.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">My 2 cents…. Maybe 3.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Greg<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#1F3864">****************<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496">Gregory J. Watkins-Colwell<o:p></o:p></span></p>
<p class="MsoNormal"><i><span style="font-size:10.0pt;color:#2F5496">Sr. Collection Manager, Herpetology and Ichthyology<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="font-size:10.0pt;color:#2F5496">Division of Vertebrate Zoology<o:p></o:p></span></i></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:red;background:white"><a href="https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Forcid.org%2F0000-0002-7789-9806&data=04%7C01%7CMilenskyC%40si.edu%7Ceaa0ff108b354f02c44d08da0cd55180%7C989b5e2a14e44efe93b78cdd5fc5d11c%7C0%7C0%7C637836407586256295%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=YpeWly1KJOdeF3tVeBMYJlnR1n36sy2nNAeC3dvkn2w%3D&reserved=0"><span style="color:red">https://orcid.org/0000-0002-7789-9806</span></a>
</span><span style="font-size:10.0pt;color:red"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496">YALE PEABODY MUSEUM OF NATURAL HISTORY<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496">Main Office: 203-432-3791; West Campus: 203-737-7568; Fax 203-432-9277<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#1F3864">Package shipping address:<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496">Greg Watkins-Colwell<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496">Division of Vertebrate Zoology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496">YALE PEABODY MUSEUM OF NATURAL HISTORY<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496">170–210 Whitney Avenue<br>
New Haven, CT 06511 USA<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#2F5496">203-432-3791<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;color:#1F3864">******************<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a>>
<b>On Behalf Of </b>Esther Dondorp<br>
<b>Sent:</b> Wednesday, March 23, 2022 5:24 AM<br>
<b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] DICE-CT on NHspecimens<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Dear colleagues,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Does anyone have experience with <span style="font-family:"Helvetica",sans-serif;color:#404040">Diffusible Iodine-based Contrast-enhanced Computed Tomography</span> (DICE-CT) performed on fluid preserved natural history specimens? I want
to know if it is really non destructive, if the specimens do stay stable for the next many years or more after this treatment? <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Many thanks!<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt">Esther Dondorp</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt">Senior Collectiebeheerder</span><o:p></o:p></p>
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