<div dir="ltr"><div class="gmail_default" style="font-family:garamond,serif;font-size:large">Hi all,</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">I thought I'd share some non-experimental observations on Lugol's solution and its effect on formalin fixed material, specifically frogs.</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">I have not used in DICE-CT. However, I have dissected muscles on hundreds of frogs, using Lugol's to improve visualization of muscles and enhance photography.</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">I used a "standard" histology-type solution of iodine and potassium iodide in 70% ethanol, not water. I'd reflect the skin to allow the stain to penetrate quickly, and did the dissections in layers, exposing deeper muscles and re-staining. I moved the specimen directly for storage alcohol to the Lugols with no rinsing. Often a specimen was re-stained several times, and destained in 70% ethanol (which was discarded after a few uses). I never noticed any desiccation or softening of the muscles or other organs such as the liver or intestines, but I was not collecting data on this.</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">I did not measure changes in pH, but I threw out the Lugols after a week to avoid potential acidification. I did not notice softening of bones, as some have seen. </div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">After muscle dissections, I would sometimes clear and stain the specimen using standard alizarin-alcian blue techniques. The specimens seemed to take up both the alizarin and alcian stains more quickly and evenly than did an untreated specimen. Perhaps this was a mordant effect of the iodine.</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">Based on these qualitative but numerous observations, as a curator I would have little reservation about allowing DICE-CT staining of specimens, but bear in mind that I was working with generally smaller vertebrates that were sufficiently stained in 3-6 hours.</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">Of course, it should be noted in the catalog that the specimen was treated in this way, to inform future researchers.</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">Best,</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large">Dave</div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div><div class="gmail_default" style="font-family:garamond,serif;font-size:large"><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Mar 23, 2022 at 9:01 AM Bentley, Andrew Charles <<a href="mailto:abentley@ku.edu">abentley@ku.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex">
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<p class="MsoNormal">Esther<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">The oVert project here in the US has had extensive experience with diceCT and have developed the attached protocol. Jaimi Gray would be the person to contact in this regard - <a href="mailto:jaimigray@floridamuseum.ufl.edu" target="_blank">jaimigray@floridamuseum.ufl.edu</a>. She also co-authored the following
publication - <a href="https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467" target="_blank">
https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467</a><u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">Hope that helps<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">Andy<u></u><u></u></p>
<p class="MsoNormal"><span lang="EN-AU"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-AU">Dyche Hall<br>
<a><span style="color:rgb(5,99,193)">1345 Jayhawk Boulevard</span></a><br>
<a><span style="color:rgb(5,99,193)">Lawrence, KS, 66045-7561</span></a><br>
<a><span style="color:rgb(5,99,193)">USA</span></a><br>
<br>
Tel: <a href="tel:%28785%29%20864-3863" target="_blank"><span style="color:rgb(5,99,193)">(785) 864-3863</span></a><br>
Fax: <a href="tel:%28785%29%20864-5335" target="_blank"><span style="color:rgb(5,99,193)">(785) 864-5335</span></a> <br>
Email: <a href="mailto:abentley@ku.edu" target="_blank"><span style="color:rgb(5,99,193)">abentley@ku.edu</span></a> <u></u><u></u></span></p>
<p class="MsoNormal">ORCID: <span style="color:rgb(73,74,76);background-color:white"><a href="https://orcid.org/0000-0002-3093-1258" target="_blank"><span style="color:rgb(5,99,193)">https://orcid.org/0000-0002-3093-1258</span></a><u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-AU"><a href="http://ichthyology.biodiversity.ku.edu/" target="_blank"><span style="color:rgb(5,99,193)">http://ichthyology.biodiversity.ku.edu</span></a></span><u></u><u></u></p>
<p class="MsoNormal"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal"><b>From:</b> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu" target="_blank">nhcoll-l-bounces@mailman.yale.edu</a>> <b>
On Behalf Of </b>Esther Dondorp<br>
<b>Sent:</b> Wednesday, March 23, 2022 4:24 AM<br>
<b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] DICE-CT on NHspecimens<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
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<p class="MsoNormal">Dear colleagues,<u></u><u></u></p>
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<p class="MsoNormal"><u></u> <u></u></p>
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<p class="MsoNormal">Does anyone have experience with <span style="font-family:Helvetica,sans-serif;color:rgb(64,64,64)">Diffusible Iodine-based Contrast-enhanced Computed Tomography</span> (DICE-CT) performed on fluid preserved natural history specimens? I want
to know if it is really non destructive, if the specimens do stay stable for the next many years or more after this treatment? <u></u><u></u></p>
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<p class="MsoNormal"><u></u> <u></u></p>
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<p class="MsoNormal">Many thanks!<u></u><u></u></p>
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<p class="MsoNormal"><u></u> <u></u></p>
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<p class="MsoNormal"><span style="font-size:10pt">Esther Dondorp</span><u></u><u></u></p>
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<p class="MsoNormal"><span style="font-size:10pt">Senior Collectiebeheerder</span><u></u><u></u></p>
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</blockquote></div><br clear="all"><div><br></div>-- <br><div dir="ltr" class="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div><div><div><div><font size="2"><span style="color:rgb(153,153,153)">David Cannatella </span></font><font size="2"><span style="color:rgb(153,153,153)"><span><font size="2"><span style="color:rgb(153,153,153)"><br>Assoc. Director, Biodiversity Center<br></span></font></span>Department of Integrative Biology<br></span></font></div><font size="2"><span style="color:rgb(153,153,153)"></span></font></div><font size="2"><span style="color:rgb(153,153,153)">Patterson Labs, 2415 Speedway<br></span></font></div><font size="2"><span style="color:rgb(153,153,153)"><span style="color:rgb(230,145,56)">University of Texas,</span> Austin, Texas 78712<br></span></font></div><font size="2"><span style="color:rgb(153,153,153)"><span style="color:rgb(204,204,204)"><a href="http://www.cannatellalab.org" target="_blank">www.cannatellalab.org</a></span><br></span></font></div><font size="2"><span style="color:rgb(153,153,153)">512.453.1620 cell</span></font><br></div></div></div></div>