<div dir="ltr"><div>In case it's helpful, here are a few papers with different concentrations for different specimens, and one that used diceCT and then did histology. It's destructive in the sense that, regardless of "de-staining" with sodium thiosulfate, you'll still end up with iodine remaining in the specimen (but it won't be red anymore!) You can at least remove the red color, and specimens are still amenable to later histology, etc. The shrinkage can be mitigated well with Sorensen's buffer, and specimens are then stable in ethanol. (I mean, it's a new-ish protocol, so who knows what they'll look like in 100 years, but I don't know why it'd be much different from any other specimen in alcohol!)<br></div><div>In addition to talking with Jaimi Gray, I also recommend talking to Catherine Early, Paul Gignac, and Aki Watanabe - all super knowledgeable about diceCT techniques for vertebrate specimens.</div><div><br></div><div>Allison</div><div><br></div><div><br></div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Mar 23, 2022 at 1:28 PM Randall, Zachary <<a href="mailto:zrandall@flmnh.ufl.edu">zrandall@flmnh.ufl.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
<div style="overflow-wrap: break-word;" lang="EN-US">
<div class="gmail-m_-6391998431288440204WordSection1">
<p class="MsoNormal">The biggest issue with diceCT has been soft-tissue shrinkage, but this has been remedied by using Sorensen’s buffer as published by Dawood et al. 2021. This is the buffer used in the Gray protocol shared by Andy. Links to the Dawood paper
as well as others of potential interest are below. <u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">Dawood et al. 2021: <a href="https://www.nature.com/articles/s41598-021-99202-2" target="_blank">
https://www.nature.com/articles/s41598-021-99202-2</a><u></u><u></u></p>
<p class="MsoNormal">Early et al. 2020: <a href="https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238783" target="_blank">
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238783</a><u></u><u></u></p>
<p class="MsoNormal">Hedrick et al. 2018: <a href="https://www.researchgate.net/publication/325862458_Assessing_Soft-Tissue_Shrinkage_Estimates_in_Museum_Specimens_Imaged_With_Diffusible_Iodine-Based_Contrast-Enhanced_Computed_Tomography_diceCT" target="_blank">
https://www.researchgate.net/publication/325862458_Assessing_Soft-Tissue_Shrinkage_Estimates_in_Museum_Specimens_Imaged_With_Diffusible_Iodine-Based_Contrast-Enhanced_Computed_Tomography_diceCT</a>
<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal">Best,<u></u><u></u></p>
<p class="MsoNormal">Zach<u></u><u></u></p>
<p class="MsoNormal"><span style="color:rgb(31,73,125)">--</span> <u></u><u></u></p>
<p class="MsoNormal"><b><span style="color:rgb(31,73,125)">Zachary S. Randall</span><u></u><u></u></b></p>
<p class="MsoNormal"><span style="color:rgb(31,73,125)">Digital Imaging Manager </span><u></u><u></u></p>
<p class="MsoNormal"><span style="color:rgb(31,73,125)">Florida Museum of Natural History
</span><u></u><u></u></p>
<p class="MsoNormal"><span style="color:rgb(31,73,125)">1659 Museum Road, Gainesville, FL 32611-7800</span>
<u></u><u></u></p>
<p class="MsoNormal"><span style="color:rgb(31,73,125)">(352) 273-1958 | <a href="mailto:zrandall@flmnh.ufl.edu" target="_blank">
zrandall@flmnh.ufl.edu</a> <u></u><u></u></span></p>
<p class="MsoNormal"><span style="color:rgb(31,73,125)"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="color:rgb(31,73,125)"><a href="https://zachrandall.weebly.com/" target="_blank">https://zachrandall.weebly.com/</a><u></u><u></u></span></p>
<p class="MsoNormal"><a href="http://www.floridamuseum.ufl.edu/digital-lab/" target="_blank">www.floridamuseum.ufl.edu/digital-lab/</a><u></u><u></u></p>
<p class="MsoNormal"><a href="https://sketchfab.com/FloridaMuseum" target="_blank">https://sketchfab.com/FloridaMuseum</a><u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<div style="border-color:rgb(225,225,225) currentcolor currentcolor;border-style:solid none none;border-width:1pt medium medium;padding:3pt 0in 0in">
<p class="MsoNormal"><b>From:</b> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu" target="_blank">nhcoll-l-bounces@mailman.yale.edu</a>> <b>
On Behalf Of </b>Bentley, Andrew Charles<br>
<b>Sent:</b> Wednesday, March 23, 2022 10:01 AM<br>
<b>To:</b> Esther Dondorp <<a href="mailto:esther.dondorp@naturalis.nl" target="_blank">esther.dondorp@naturalis.nl</a>>; <a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> Re: [Nhcoll-l] DICE-CT on NHspecimens<u></u><u></u></p>
</div>
</div>
<p class="MsoNormal"><u></u> <u></u></p>
<table style="width:100%" width="100%" cellspacing="0" cellpadding="0" border="0" align="left">
<tbody>
<tr>
<td style="padding:0in">
<div>
<p class="MsoNormal">
<b><span style="font-size:9pt;font-family:"Arial",sans-serif;color:rgb(250,70,22)">[External Email]</span></b>
<u></u><u></u></p>
</div>
</td>
</tr>
</tbody>
</table>
<div>
<p class="MsoNormal"><span style="color:black">Esther</span><u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">The oVert project here in the US has had extensive experience with diceCT and have developed the attached protocol. Jaimi Gray would be the person to contact in this regard - <a href="mailto:jaimigray@floridamuseum.ufl.edu" target="_blank">jaimigray@floridamuseum.ufl.edu</a>. She also co-authored the following
publication - <a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__onlinelibrary.wiley.com_doi_full_10.1002_ece3.7467&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=PCoqPP0RplincQmDRfDfKbAf08gmtmu0zMnevmF7kYE&e=" target="_blank">
https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467</a><u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">Hope that helps<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">Andy<u></u><u></u></p>
<p class="MsoNormal"><span lang="EN-AU"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-AU">Dyche Hall<br>
<a><span style="color:rgb(5,99,193)">1345 Jayhawk Boulevard</span></a><br>
<a><span style="color:rgb(5,99,193)">Lawrence, KS, 66045-7561</span></a><br>
<a><span style="color:rgb(5,99,193)">USA</span></a><br>
<br>
Tel: <a href="tel:%28785%29%20864-3863" target="_blank"><span style="color:rgb(5,99,193)">(785) 864-3863</span></a><br>
Fax: <a href="tel:%28785%29%20864-5335" target="_blank"><span style="color:rgb(5,99,193)">(785) 864-5335</span></a> <br>
Email: <a href="mailto:abentley@ku.edu" target="_blank"><span style="color:rgb(5,99,193)">abentley@ku.edu</span></a> <u></u><u></u></span></p>
<p class="MsoNormal">ORCID: <span style="color:rgb(73,74,76);background:white none repeat scroll 0% 0%"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__orcid.org_0000-2D0002-2D3093-2D1258&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=W6K85Ze9PmgioL9GHoEqKgA6yf-ISXmJ6QyjpUy0Uyw&e=" target="_blank"><span style="color:rgb(5,99,193)">https://orcid.org/0000-0002-3093-1258</span></a><u></u><u></u></span></p>
<p class="MsoNormal"><span lang="EN-AU"><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu_&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=ANS6f0lN9_8S28FjQKr0jwy3geQCl1FB4n7T1ALKhwg&e=" target="_blank"><span style="color:rgb(5,99,193)">http://ichthyology.biodiversity.ku.edu</span></a></span><u></u><u></u></p>
<p class="MsoNormal"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal"><b>From:</b> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu" target="_blank">nhcoll-l-bounces@mailman.yale.edu</a>> <b>
On Behalf Of </b>Esther Dondorp<br>
<b>Sent:</b> Wednesday, March 23, 2022 4:24 AM<br>
<b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] DICE-CT on NHspecimens<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal">Dear colleagues,<u></u><u></u></p>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Does anyone have experience with <span style="font-family:"Helvetica",sans-serif;color:rgb(64,64,64)">Diffusible Iodine-based Contrast-enhanced Computed Tomography</span> (DICE-CT) performed on fluid preserved natural history specimens? I want
to know if it is really non destructive, if the specimens do stay stable for the next many years or more after this treatment? <u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Many thanks!<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<div>
<div>
<div>
<p class="MsoNormal"><span style="font-size:10pt">Esther Dondorp</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:10pt">Senior Collectiebeheerder</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__nam10.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Fnaturalis-2Dpublic-2Dmedia-2Dassets.s3.eu-2Dcentral-2D1.amazonaws.com-252FNaturalis-2Dmail-2Dsignature-252Flogo.png-26data-3D04-257C01-257Cabentley-2540ku.edu-257C045b40042878448dbb0208da0caef50d-257C3c176536afe643f5b96636feabbe3c1a-257C0-257C0-257C637836242826560921-257CUnknown-257CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0-253D-257C3000-26sdata-3D9F3QxE2YraYusdBUWfenHfsfCdHXPhXAQ4PcmIBbZkk-253D-26reserved-3D0&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=RYSSbVNkyqIqkc1C-VlPbEfSBXyqzx7etNMkIzMCpGk&e=" title="Naturalis-logo" target="_blank"><span style="font-family:"Arial",sans-serif;border:1pt solid windowtext;padding:0in;text-decoration:none"><img style="width: 1.0416in; height: 1.0416in;" id="gmail-m_-6391998431288440204Picture_x0020_1" src="cid:17fbd50bb67bf2963031" alt="Image removed by sender." width="100" height="100" border="0"></span></a><u></u><u></u></p>
</div>
<p><br>
<br>
<br>
<br>
<br>
<sub><span style="font-size:8pt">- -</span></sub><br>
<sub><span style="font-size:8pt"><a href="mailto:esther.dondorp@naturalis.nl" target="_blank">esther.dondorp@naturalis.nl</a> -
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__nam10.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Fwww.naturalis.nl-252F-26data-3D04-257C01-257Cabentley-2540ku.edu-257C045b40042878448dbb0208da0caef50d-257C3c176536afe643f5b96636feabbe3c1a-257C0-257C0-257C637836242826560921-257CUnknown-257CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0-253D-257C3000-26sdata-3Dy-252B43gsCV3bwJk4g4Wdm06Z8fbmHMM1HfR20aqEGlWBQ-253D-26reserved-3D0&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=adbMMe-pIUO8NhDu0IUIdOuKJcJMbH9yn8KRx5h8nVY&e=" target="_blank">
www.naturalis.nl</a></span></sub><br>
<sub><span style="font-size:8pt">Darwinweg 2, 2333 CR Leiden</span></sub><br>
<sub><span style="font-size:8pt">Postbus 9517, 2300 RA Leiden</span></sub><u></u><u></u></p>
<div>
<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__nam10.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Fwww.naturalis.nl-252F-26data-3D04-257C01-257Cabentley-2540ku.edu-257C045b40042878448dbb0208da0caef50d-257C3c176536afe643f5b96636feabbe3c1a-257C0-257C0-257C637836242826560921-257CUnknown-257CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0-253D-257C3000-26sdata-3Dy-252B43gsCV3bwJk4g4Wdm06Z8fbmHMM1HfR20aqEGlWBQ-253D-26reserved-3D0&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=adbMMe-pIUO8NhDu0IUIdOuKJcJMbH9yn8KRx5h8nVY&e=" target="_blank"><span style="border:1pt solid windowtext;padding:0in;text-decoration:none"><img style="width: 1.0416in; height: 1.0416in;" id="gmail-m_-6391998431288440204Picture_x0020_2" src="cid:17fbd50bb67bf2963031" alt="Image removed by sender." width="100" height="100" border="0"></span></a><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"> <u></u><u></u></p>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
_______________________________________________<br>
Nhcoll-l mailing list<br>
<a href="mailto:Nhcoll-l@mailman.yale.edu" target="_blank">Nhcoll-l@mailman.yale.edu</a><br>
<a href="https://mailman.yale.edu/mailman/listinfo/nhcoll-l" rel="noreferrer" target="_blank">https://mailman.yale.edu/mailman/listinfo/nhcoll-l</a><br>
<br>
_______________________________________________<br>
NHCOLL-L is brought to you by the Society for the Preservation of<br>
Natural History Collections (SPNHC), an international society whose<br>
mission is to improve the preservation, conservation and management of<br>
natural history collections to ensure their continuing value to<br>
society. See <a href="http://www.spnhc.org" rel="noreferrer" target="_blank">http://www.spnhc.org</a> for membership information.<br>
Advertising on NH-COLL-L is inappropriate.<br>
</blockquote></div><br clear="all"><br>-- <br><div dir="ltr" class="gmail_signature"><div dir="ltr">Dr. Allison W. Bronson<div><div>Adjunct Faculty and Research Associate</div></div><div>Department of Biological Sciences</div><div>California State Polytechnic University, Humboldt<br></div><div><a href="https://sites.google.com/humboldt.edu/fish-anatomy" target="_blank">https://sites.google.com/humboldt.edu/fish-anatomy</a><br></div></div></div>