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<p class="MsoNormal">Hi Tonya<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">It is true that frozen specimens do not make ideal ethanol specimens as the formalin and ethanol do not pervade the tissues as well when frozen an thawed. You can counteract some of that by injecting with formalin into all body cavities
and leaving it soaking in formalin for a little longer. It also helps to step the specimen up through concentrations of ethanol to ensure good preservation throughout the specimen.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Andy<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU">Dyche Hall<br>
<a href="x-apple-data-detectors://9/">1345 Jayhawk Boulevard</a><br>
<a href="x-apple-data-detectors://9/">Lawrence, KS, 66045-7561</a><br>
<a href="x-apple-data-detectors://9/">USA</a><br>
<br>
Tel: <a href="tel:%28785%29%20864-3863" target="_blank">(785) 864-3863</a><br>
Fax: <a href="tel:%28785%29%20864-5335" target="_blank">(785) 864-5335</a> <br>
Email: <a href="mailto:abentley@ku.edu" target="_blank">abentley@ku.edu</a> <o:p></o:p></span></p>
<p class="MsoNormal">ORCID: <span style="color:#494A4C;background:white"><a href="https://orcid.org/0000-0002-3093-1258">https://orcid.org/0000-0002-3093-1258</a><o:p></o:p></span></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="EN-AU"><a href="http://ichthyology.biodiversity.ku.edu/" target="_blank">http://ichthyology.biodiversity.ku.edu</a></span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> <b>
On Behalf Of </b>Haff, Tonya (NCMI, Crace)<br>
<b>Sent:</b> Tuesday, April 26, 2022 11:26 PM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [Nhcoll-l] prepping frozen herps<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="EN-AU">Hello all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">I have read (in John Simmon’s book on herpetological collecting, among other places) that frozen snake and lizard specimens do not make suitable formalin-preserved specimens, and should be instead skeletonised. We have
quite a few herp specimens in the freezer that we have been planning on prepping as ‘pickles’ (fixed in formalin and then stored in ETOH), but I haven’t started yet because of the concern that it may not be worthwhile. I wonder if any of you could weigh in
on this and tell me what your experiences have been, and whether or not you would bother preserving these specimens in spirit, or if we should just prep them as skeletons?<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Thanks!<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Tonya<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">-------------------------------------------------<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Dr. Tonya M. Haff<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Collection Manager<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Australian National Wildlife Collection<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">CSIRO<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
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