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<p class="MsoNormal"><span style="mso-fareast-language:JA">We cut 95% ethanol to 80% in 25-liter carboys, rolling them on their sides to mix. I regularly test the fluid during use, and it holds steady at 80-81% (I just tested the remains of a batch mixed in
March; 80.6 originally, 81.0 now in a sample drawn through the spigot; presumably slightly lower at the surface). I haven’t, therefore, seen major re-separation, though I’m aware (thanks to John’s work!) of the properties of binary azeotropes. Could it be,
I wonder, that the slightly higher concentration we use helps avoid this problem? We switched from 70% to 80% about 20 years ago.
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<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black;mso-fareast-language:JA">Paul Callomon<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black;mso-fareast-language:JA">Collection Manager, Malacology and General Invertebrates<i><o:p></o:p></i></span></p>
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<p class="MsoNormal" style="background:white"><b><span style="font-size:12.0pt;color:black;mso-fareast-language:JA">Academy of Natural Sciences of Drexel University</span></b><b><span style="font-size:12.0pt;color:black;mso-fareast-language:JA"><o:p></o:p></span></b></p>
<p class="MsoNormal" style="background:white"><span style="font-size:12.0pt;color:black;mso-fareast-language:JA">1900 Benjamin Franklin Parkway, Philadelphia PA 19103-1195, USA<br>
<i><a href="mailto:prc44@drexel.edu">prc44@drexel.edu</a> Tel 215-405-5096 - Fax 215-299-1170</i><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-left:.5in"><b><span style="mso-fareast-language:JA">From:</span></b><span style="mso-fareast-language:JA"> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu>
<b>On Behalf Of </b>Haff, Tonya (NCMI, Crace)<br>
<b>Sent:</b> Tuesday, September 6, 2022 9:40 PM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [Nhcoll-l] Mixing EtOH<o:p></o:p></span></p>
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<p><b><span style="font-family:"Arial",sans-serif;font-variant:small-caps;color:black">External.</span></b><b><span style="font-family:"Arial",sans-serif;font-variant:small-caps"><o:p></o:p></span></b></p>
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<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU">Hello all,<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU">I have a question about mixing storage strength (70%) undenatured EtOH. Typically we add the correct proportions of 95% EtOH and water to a container and paddle or invert the container repeatedly
to mix the liquids, and allow it to sit for ~24+ hrs. The containers we like best to use for dispensing 70% EtOH are 20L plastic water containers with a tap at the bottom. Recently we used our digital alcohol meter to test the alcohol concentration from the
top and bottom (tap) of one of these containers and found the alcohol concentrations wildly different - ~80% at the top and ~60% at the bottom – despite having been mixed more than 24 hours earlier. This makes me really concerned that we could be regularly
using concentrations that are much above 70% with specimens. I wonder if any of you have had a similar problem, or if anyone can suggest a solution? Is there a better way of mixing or of ensuring the solution is properly combined? Any thoughts appreciated.<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU">Thank you!<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU">Cheers,<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU">Tonya<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU" style="mso-fareast-language:EN-AU">-------------------------------------------------<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Dr. Tonya M. Haff<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Collection Manager<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Australian National Wildlife Collection<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU" style="mso-fareast-language:EN-AU">CSIRO<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU" style="mso-fareast-language:EN-AU">+61(0)419569109<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:.5in"><span lang="EN-AU" style="mso-fareast-language:EN-AU"><a href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.csiro.au%2Fen%2Fabout%2Ffacilities-collections%2Fcollections%2Fanwc&data=05%7C01%7Cprc44%40drexel.edu%7Cd03592d84dd74241473408da9071dc8a%7C3664e6fa47bd45a696708c4f080f8ca6%7C0%7C0%7C637981115973263441%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=K%2F95ao2%2FCHvq5tt%2FfPjFqZ0ORcgpA8z54x3HB1fI284%3D&reserved=0">https://www.csiro.au/en/about/facilities-collections/collections/anwc</a><o:p></o:p></span></p>
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