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<p class="MsoNormal">I just thought I’d add a comment on trying to preserve colour by embedding in resin. All resins yellow and discolour over time, so will affect the colour of what you are looking at in them. Polyester is likely to be visibly yellowed within
20 years unless kept in cold, dark, anoxic conditions. You’re better off keeping the specimen in cold, dark, anoxic conditions. Different resins age at different rates, but all of them age.<o:p></o:p></p>
<p class="MsoNormal">Valerie Tomlinson<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu>
<b>On Behalf Of </b>a.j.van_dam@lumc.nl<br>
<b>Sent:</b> Sunday, September 18, 2022 1:40 PM<br>
<b>To:</b> d.neumann@leibniz-lib.de; cutraccimaxine@gmail.com; nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [EXT]Re: [Nhcoll-l] [MOGELIJK SPAM ! *****] Preserve fish specimens<o:p></o:p></p>
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<p><span style="font-size:12.0pt;color:black">Hi Dirk,<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black">Preserving the color of living fish does not seem to me a problem at all, as long as you nurture them well.
</span><span style="font-size:12.0pt;font-family:"Segoe UI Emoji",sans-serif;color:black">😉</span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black">I think the question of Maxine is (@Maxine, am I right?): What is the best fixation/preservation method to prevent further colour loss post-mortem. Glycerol preservation in combination with embedding in polyester
could certainly be worthwhile to try out.<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black">I experienced myself some good results with regard to colour with the preservation of small sharks on 65% glycerol. In case of small fish (5-10 mm in length), fixation and preservation steps can be very short and
thus giving quick results for comparison to the standard preservation fluids (ethanol/formalin). Additional embedding in polyester might even further stabilize the colour.<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black">Regards, Dries<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="NL" style="font-size:8.0pt;line-height:106%;font-family:"Verdana",sans-serif;color:black"><a href="http://www.linkedin.com/in/andriesvandam">Andries J. van Dam</a> |
curator-conservator</span><span style="font-size:10.0pt;line-height:106%;font-family:"Tahoma",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="color:black">Van:</span></b><span style="color:black"> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu">nhcoll-l-bounces@mailman.yale.edu</a>> namens Dirk Neumann <<a href="mailto:d.neumann@leibniz-lib.de">d.neumann@leibniz-lib.de</a>><br>
<b>Verzonden:</b> zondag 18 september 2022 17:11:25<br>
<b>Aan:</b> <a href="mailto:cutraccimaxine@gmail.com">cutraccimaxine@gmail.com</a>;
<a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br>
<b>Onderwerp:</b> Re: [Nhcoll-l] [MOGELIJK SPAM ! *****] Preserve fish specimens</span>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal">Dear Maxine,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">it is very difficult to preserve the colour of living fish; even if you follow Dries' recommendation and recipe, the colour in the preserved specimen will not be the same as in the living one. If the colour carries important information,
e.g. to characterise or determine the species, it would be best to photograph the still living fish in a small photo tank, because as soon as the fish is dead, the colour usually fade within seconds or minutes.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">High resolution images allow to zoom in an even can give nuances of different melanophores. If you need this colour information for your research, you should make sure that you take the photos in a standardised setting.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">The SPNHC Wiki pages offer further infromation that might be useful
<a href="https://spnhc.biowikifarm.net/wiki/Digital_Imaging">https://spnhc.biowikifarm.net/wiki/Digital_Imaging</a>, at the end there are links that direct you to specific sites/PDFs that provide further details on the setup.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Hope this helps<o:p></o:p></p>
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<p class="MsoNormal">Dirk<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Am 18.09.2022 um 13:13 schrieb <a href="mailto:a.j.van_dam@lumc.nl">
a.j.van_dam@lumc.nl</a>:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black">Dear Maxine,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black">You could indeed do some experiments with glycerol to see if it would better preserve the colours. To avoid shrinkage, it is important that after fixation you transfer to glycerol
by increasing concentration steps (e.g. 30-50-70%). A concentration between 65-70% should be sufficient to keep them well preserved and prevent fungal growth, provided that the RH in the room where the jars are kept does not exceed 70%.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black">You might also want to embed them after a complete glycerol transfer (30-50-70-80-90-95-100-100%) in polyester resin. Before embedding them, you should get most of the glycerol
off the surface by using paper or cotton towels and cotton swabs. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black">Just before placing them in the unhardened polyester (3 layers: ground, middle embedding layer, and top layer), dip the specimens for about a minute or so in acetone and then cellosolve.
This ensures that the polyester will bind to the skin of the fish. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black">Kind regards,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black">Dries<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:12.0pt;line-height:106%;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span lang="NL" style="font-size:8.0pt;line-height:106%;font-family:"Verdana",sans-serif;color:black"><a href="http://www.linkedin.com/in/andriesvandam">Andries J. van Dam</a> |
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<p class="MsoNormal"><b><span style="color:black">Van:</span></b><span style="color:black"> Nhcoll-l
<a href="mailto:nhcoll-l-bounces@mailman.yale.edu"><nhcoll-l-bounces@mailman.yale.edu></a> namens Maxine C
<a href="mailto:cutraccimaxine@gmail.com"><cutraccimaxine@gmail.com></a><br>
<b>Verzonden:</b> vrijdag 16 september 2022 05:40:43<br>
<b>Aan:</b> <a href="mailto:nhcoll-l@mailman.yale.edu">nhcoll-l@mailman.yale.edu</a><br>
<b>Onderwerp:</b> [MOGELIJK SPAM ! *****] [Nhcoll-l] Preserve fish specimens</span>
<o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal">Hi all, <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I'm working on a research project at the University of Hong Kong on fish biodiversity. We would like to preserve very small cryptobenthic fish species ( 5 - 10 mm in length). <o:p></o:p></p>
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<p class="MsoNormal">In the past, I've used formalin and ethanol 70% but I'd like to preserve the coloration.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Any advice? Would resin work?<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Regards,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Maxine <o:p></o:p></p>
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<p class="MsoNormal"><br>
<br>
<o:p></o:p></p>
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<pre><a href="mailto:Nhcoll-l@mailman.yale.edu">Nhcoll-l@mailman.yale.edu</a><o:p></o:p></pre>
<pre><a href="https://mailman.yale.edu/mailman/listinfo/nhcoll-l">https://mailman.yale.edu/mailman/listinfo/nhcoll-l</a><o:p></o:p></pre>
<pre><o:p> </o:p></pre>
<pre>_______________________________________________<o:p></o:p></pre>
<pre>NHCOLL-L is brought to you by the Society for the Preservation of<o:p></o:p></pre>
<pre>Natural History Collections (SPNHC), an international society whose<o:p></o:p></pre>
<pre>mission is to improve the preservation, conservation and management of<o:p></o:p></pre>
<pre>natural history collections to ensure their continuing value to<o:p></o:p></pre>
<pre>society. See <a href="http://www.spnhc.org">http://www.spnhc.org</a> for membership information.<o:p></o:p></pre>
<pre>Advertising on NH-COLL-L is inappropriate.<o:p></o:p></pre>
</blockquote>
<p><o:p> </o:p></p>
<div>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal">-- <o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><b><span style="font-family:"Source Sans Pro ,sans-serif",serif"> </span></b><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><b><span style="font-family:"Source Sans Pro ,sans-serif",serif">Dirk Neumann</span></b><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><span style="font-family:"Source Sans Pro ,sans-serif",serif">Collection Manager, Hamburg</span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><span style="font-family:"Source Sans Pro ,sans-serif",serif"> </span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><span style="font-family:"Source Sans Pro ,sans-serif",serif">Postal address:</span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><b><span style="font-family:"Source Sans Pro ,sans-serif",serif">Museum of Nature Hamburg</span></b><span style="font-family:"Source Sans Pro",sans-serif"><br>
</span>Leibniz Institute for the Analysis<o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal">of Biodiversity Change<o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><span style="font-family:"Source Sans Pro ,sans-serif",serif">Dirk Neumann</span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><span style="font-family:"Source Sans Pro ,sans-serif",serif">Martin-Luther-King-Platz 3</span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><span style="font-family:"Source Sans Pro ,sans-serif",serif">20146</span><span style="font-family:"Source Sans Pro",sans-serif">
</span><span style="font-family:"Source Sans Pro ,sans-serif",serif">Hamburg</span><span style="font-family:"Source Sans Pro",sans-serif"><br>
+49 </span><span style="font-family:"Source Sans Pro ,sans-serif",serif">40</span><span style="font-family:"Source Sans Pro",sans-serif">
</span><span style="font-family:"Source Sans Pro ,sans-serif",serif">238 3</span><span style="font-family:"Source Sans Pro",sans-serif">17 –
</span><span style="font-family:"Source Sans Pro ,sans-serif",serif">628</span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><a href="mailto:d.neumann@lebniz-lib.de"><span style="font-family:"Source Sans Pro",sans-serif">d.neumann@lebniz-lib.de</span></a><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><a href="imap://dneumann@webmail.leibniz-lib.de:993/fetch%3EUID%3E/INBOX/Privat/www.leibniz-lib.de"><span style="font-family:"Source Sans Pro",sans-serif">www.leibniz-lib.de</span></a><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><span style="font-size:10.0pt;font-family:"Source Sans Pro ,sans-serif",serif"> </span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal"><span style="font-size:10.0pt;font-family:"Source Sans Pro ,sans-serif",serif">--
<br>
Stiftung Leibniz-Institut zur Analyse des Biodiversitätswandels<br>
Postanschrift: Adenauerallee 127, 53113 Bonn, Germany<br>
<br>
Stiftung des öffentlichen Rechts;<br>
Generaldirektion: Prof. Dr. Bernhard Misof (Generaldirektor), Adrian Grüter (Kaufm. Geschäftsführer)<br>
Sitz der Stiftung: Adenauerallee 160 in Bonn<br>
Vorsitzender des Stiftungsrates: Dr. Michael Wappelhorst</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt;line-height:106%;font-family:"Source Sans Pro",sans-serif"> </span><o:p></o:p></p>
</div>
<p class="MsoNormal" style="margin-bottom:0in;line-height:normal">-- <br>
Stiftung Leibniz-Institut zur Analyse des Biodiversitätswandels<br>
Postanschrift: Adenauerallee 127, 53113 Bonn, Germany<br>
<br>
Stiftung des öffentlichen Rechts;<br>
Generaldirektion: Prof. Dr. Bernhard Misof (Generaldirektor), Adrian Grüter (Kaufm. Geschäftsführer)<br>
Sitz der Stiftung: Adenauerallee 160 in Bonn<br>
Vorsitzender des Stiftungsrates: Dr. Michael Wappelhorst<o:p></o:p></p>
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