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<p>Hey Tonya,</p>
<p>I would recommend to measure the density of the solution before
assuming anything. With this you can calculate what percentage of
strength you can expect. Usually this can vary very much from
literature, to literature. And i really have never heard the use
of ethanol in clearings. The ones I have made are all in plain
glycerol and water and are preserved very well.<br>
</p>
<p>And I would not add any thymol, since if the solution is above
65% mold growth is inhibited. Why use poison if not needed...</p>
<p><br>
</p>
<p>All the best,<br>
</p>
<p><br>
</p>
<div class="moz-cite-prefix">Am 06.10.22 um 04:32 schrieb Bentley,
Andrew Charles:<br>
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<p class="MsoNormal">Tonya<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We have sometimes mixed different
proportions of glycerin and 70% ethanol to make the liquid
less viscous and thus reduce bubble formation in cavities of
C&S specimens but otherwise use full strength glycerin for
these specimens with a couple of crystals of thymol added to
prevent mold growth. The addition of 70% ethanol, as long as
not too much, does not seem to affect these specimens in any
adverse way – that we can tell.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Andy<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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A : A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<br>
Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black" lang="EN-AU">Dyche
Hall<br>
<a href="x-apple-data-detectors://9/"
title="x-apple-data-detectors://9/"
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KS, 66045-7561</span></a><br>
<a href="x-apple-data-detectors://9/"
title="x-apple-data-detectors://9/"
moz-do-not-send="true"><span style="color:#954F72">USA</span></a><br>
<br>
Tel: <a href="tel:%28785%29%20864-3863" target="_blank"
title="tel:%28785%29%20864-3863"
moz-do-not-send="true"><span style="color:#954F72">(785)
864-3863</span></a><br>
Fax: <a href="tel:%28785%29%20864-5335" target="_blank"
title="tel:%28785%29%20864-5335"
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Email: <a href="mailto:abentley@ku.edu" target="_blank"
title="mailto:abentley@ku.edu" moz-do-not-send="true"><span
style="color:#954F72">abentley@ku.edu</span></a> </span><span
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<p class="MsoNormal"><span style="color:black">ORCID: </span><span
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href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Forcid.org%2F0000-0002-3093-1258&data=04%7C01%7Cabentley%40ku.edu%7C5720c0e4d0f94b9e0df008da0127752c%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637823566221065460%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=LFnPbiFA9HMZ8k85%2F0%2FOx5vJxTNhZTjAo7S%2BqrDAh6c%3D&reserved=0"
title="Original URL:
https://orcid.org/0000-0002-3093-1258
Click to follow link." moz-do-not-send="true"><span
style="color:#954F72">https://orcid.org/0000-0002-3093-1258</span></a></span><span
style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black" lang="EN-AU"><a
href="http://ichthyology.biodiversity.ku.edu/"
target="_blank"
title="http://ichthyology.biodiversity.ku.edu/"
moz-do-not-send="true"><span style="color:#954F72">http://ichthyology.biodiversity.ku.edu</span></a></span><span
style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black"> A
: A : A :<br>
}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
V V V<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<div style="border:none;border-top:solid #B5C4DF
1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span
style="font-size:12.0pt;color:black" lang="EN-AU">From:
</span></b><span style="font-size:12.0pt;color:black"
lang="EN-AU">Nhcoll-l
<a class="moz-txt-link-rfc2396E" href="mailto:nhcoll-l-bounces@mailman.yale.edu"><nhcoll-l-bounces@mailman.yale.edu></a> on behalf of
"Haff, Tonya (NCMI, Crace)" <a class="moz-txt-link-rfc2396E" href="mailto:Tonya.Haff@csiro.au"><Tonya.Haff@csiro.au></a><br>
<b>Date: </b>Wednesday, October 5, 2022 at 6:13 PM<br>
<b>To: </b><a class="moz-txt-link-rfc2396E" href="mailto:nhcoll-l@mailman.yale.edu">"nhcoll-l@mailman.yale.edu"</a>
<a class="moz-txt-link-rfc2396E" href="mailto:nhcoll-l@mailman.yale.edu"><nhcoll-l@mailman.yale.edu></a><br>
<b>Subject: </b>[Nhcoll-l] cleared and stained amphibians<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
</div>
<p class="MsoNormal"><span lang="EN-AU">Hello all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">We have some cleared and
stained small frogs that are, I suspect, stored in a mixture
of glycerine and ethanol. I have read in John’s Fluid
Preservation book that some people use a mixture the two at
a 1:1 ratio (but at what ETOH concentration I am not sure),
but I wonder if any of you would share how you store similar
specimens? Some of the jars need to be topped up, so I also
wonder if it’s best to top up using a 1:1 ratio of glycerine
to ethanol (given that’s what it *<b>looks</b>* like, ha
ha), or remove all fluid and replace, or…? Thoughts, as
usual, appreciated!<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Cheers,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Tonya<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU"
lang="EN-AU">-------------------------------------------------<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU"
lang="EN-AU">Dr. Tonya M. Haff<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU"
lang="EN-AU">Collection Manager<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU"
lang="EN-AU">Australian National Wildlife Collection<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU"
lang="EN-AU">CSIRO<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU"
lang="EN-AU">+61(0)419569109<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:EN-AU"
lang="EN-AU"><a
href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.csiro.au%2Fen%2Fabout%2Ffacilities-collections%2Fcollections%2Fanwc&data=05%7C01%7Cabentley%40ku.edu%7C0ef503da722a44a5569c08daa727284c%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C638006083885233690%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=xo7oQ1sFwdNOMO0Cpp68SkGfv2l0GX0YAd06SULP9mc%3D&reserved=0"
moz-do-not-send="true">https://www.csiro.au/en/about/facilities-collections/collections/anwc</a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
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