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<p class="MsoNormal">Tonya<o:p></o:p></p>
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<p class="MsoNormal">We have sometimes mixed different proportions of glycerin and 70% ethanol to make the liquid less viscous and thus reduce bubble formation in cavities of C&S specimens but otherwise use full strength glycerin for these specimens with a
couple of crystals of thymol added to prevent mold growth. The addition of 70% ethanol, as long as not too much, does not seem to affect these specimens in any adverse way – that we can tell.<o:p></o:p></p>
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<p class="MsoNormal">Andy<o:p></o:p></p>
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}<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°><br>
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Andy Bentley<br>
Ichthyology Collection Manager<br>
University of Kansas<br>
Biodiversity Institute</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="color:black">Dyche Hall<br>
<a href="x-apple-data-detectors://9/" title="x-apple-data-detectors://9/"><span style="color:#954F72">1345 Jayhawk Boulevard</span></a><br>
<a href="x-apple-data-detectors://9/" title="x-apple-data-detectors://9/"><span style="color:#954F72">Lawrence, KS, 66045-7561</span></a><br>
<a href="x-apple-data-detectors://9/" title="x-apple-data-detectors://9/"><span style="color:#954F72">USA</span></a><br>
<br>
Tel: <a href="tel:%28785%29%20864-3863" target="_blank" title="tel:%28785%29%20864-3863"><span style="color:#954F72">(785) 864-3863</span></a><br>
Fax: <a href="tel:%28785%29%20864-5335" target="_blank" title="tel:%28785%29%20864-5335"><span style="color:#954F72">(785) 864-5335</span></a> <br>
Email: <a href="mailto:abentley@ku.edu" target="_blank" title="mailto:abentley@ku.edu"><span style="color:#954F72">abentley@ku.edu</span></a> </span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">ORCID: </span><span style="color:#494A4C;background:white"><a href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Forcid.org%2F0000-0002-3093-1258&data=04%7C01%7Cabentley%40ku.edu%7C5720c0e4d0f94b9e0df008da0127752c%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637823566221065460%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=LFnPbiFA9HMZ8k85%2F0%2FOx5vJxTNhZTjAo7S%2BqrDAh6c%3D&reserved=0" title="Original URL:
https://orcid.org/0000-0002-3093-1258
Click to follow link."><span style="color:#954F72">https://orcid.org/0000-0002-3093-1258</span></a></span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="color:black"><a href="http://ichthyology.biodiversity.ku.edu/" target="_blank" title="http://ichthyology.biodiversity.ku.edu/"><span style="color:#954F72">http://ichthyology.biodiversity.ku.edu</span></a></span><span style="color:black"><o:p></o:p></span></p>
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V V V<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-AU" style="font-size:12.0pt;color:black">From:
</span></b><span lang="EN-AU" style="font-size:12.0pt;color:black">Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> on behalf of "Haff, Tonya (NCMI, Crace)" <Tonya.Haff@csiro.au><br>
<b>Date: </b>Wednesday, October 5, 2022 at 6:13 PM<br>
<b>To: </b>"nhcoll-l@mailman.yale.edu" <nhcoll-l@mailman.yale.edu><br>
<b>Subject: </b>[Nhcoll-l] cleared and stained amphibians<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-AU">Hello all,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">We have some cleared and stained small frogs that are, I suspect, stored in a mixture of glycerine and ethanol. I have read in John’s Fluid Preservation book that some people use a mixture the two at a 1:1 ratio (but
at what ETOH concentration I am not sure), but I wonder if any of you would share how you store similar specimens? Some of the jars need to be topped up, so I also wonder if it’s best to top up using a 1:1 ratio of glycerine to ethanol (given that’s what it
*<b>looks</b>* like, ha ha), or remove all fluid and replace, or…? Thoughts, as usual, appreciated!<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Cheers,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU">Tonya<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">-------------------------------------------------<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Dr. Tonya M. Haff<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Collection Manager<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">Australian National Wildlife Collection<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">CSIRO<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU">+61(0)419569109<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-AU" style="mso-fareast-language:EN-AU"><a href="https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.csiro.au%2Fen%2Fabout%2Ffacilities-collections%2Fcollections%2Fanwc&data=05%7C01%7Cabentley%40ku.edu%7C0ef503da722a44a5569c08daa727284c%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C638006083885233690%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=xo7oQ1sFwdNOMO0Cpp68SkGfv2l0GX0YAd06SULP9mc%3D&reserved=0">https://www.csiro.au/en/about/facilities-collections/collections/anwc</a><o:p></o:p></span></p>
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