<div dir="ltr"><div>How long did you leave them at -25C? Duration may be the issue. Cycling through freeze/thaw may actually allow larvae or pupae to better survive, but 14-21 days at -20C should be sufficient to kill all stages of <i>Tineola</i>. Also take into account how long it may take for the interior of the object to reach -20, so your clock shouldn't start until the interior is at temp. I don't know whether or not freezing is good for baleen though.<br></div><div><br></div><div>Chris<br></div><div><br></div><div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:10pt;font-family:Arial;color:rgb(0,120,122);font-weight:700;vertical-align:baseline;white-space:pre-wrap">Christopher C. Grinter</span></div><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:8.5pt;font-family:Arial;color:rgb(0,120,122);vertical-align:baseline;white-space:pre-wrap">Collection Manager of Entomology</span></div><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:8.5pt;font-family:Arial;color:rgb(0,120,122);vertical-align:baseline;white-space:pre-wrap">Secretary, The Lepidopterists’ Society</span></div><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:8.5pt;font-family:Arial;color:rgb(0,120,122);vertical-align:baseline;white-space:pre-wrap">Subject Editor: Lepidoptera, The Pacific Coast Entomological Society</span></div><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.7999999999999998;margin-top:0pt;margin-bottom:0pt"><span style="font-size:9.5pt;font-family:Arial;color:rgb(138,138,138);vertical-align:baseline;white-space:pre-wrap">California Academy of Sciences</span></div><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.7999999999999998;margin-top:0pt;margin-bottom:0pt"><span style="font-size:9.5pt;font-family:Arial;color:rgb(138,138,138);vertical-align:baseline;white-space:pre-wrap">55 Music Concourse Dr</span></div><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.7999999999999998;margin-top:0pt;margin-bottom:0pt"><span style="font-size:9.5pt;font-family:Arial;color:rgb(138,138,138);vertical-align:baseline;white-space:pre-wrap">San Francisco, CA 94118</span></div><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.7999999999999998;margin-top:0pt;margin-bottom:0pt"><span style="font-size:9.5pt;font-family:Arial;color:rgb(138,138,138);vertical-align:baseline;white-space:pre-wrap">T 415.379.5320</span></div><div style="color:rgb(0,0,0);font-family:"Palatino Linotype";font-size:12px;font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px;text-decoration:none;line-height:1.38;margin-top:0pt;margin-bottom:0pt"><span style="font-size:9.5pt;font-family:Arial;color:rgb(22,101,114);vertical-align:baseline;white-space:pre-wrap">We regenerate the natural world through science, learning, and collaboration.</span></div><span style="font-style:normal;font-weight:400;letter-spacing:normal;text-align:start;text-indent:0px;text-transform:none;word-spacing:0px;text-decoration:none;font-size:9.5pt;font-family:Arial;col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