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Dear Robyn,<br>
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There is a positive correlation between the depth of tissue penetration and the square root of time for fixatives/preservatives. Assuming ethanol would penetrate 1 mm into the tissue in 1 hour (some papers mention this figure for 100% ethanol as the penetration
coefficient), it would penetrate in 25 hours (ca. 1 day) 5 mm, in 49 hours (ca. 2 days) 7 mm, and in 169 hours (ca. one week) 13 mm.</div>
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Of course, besides the thickness of the tissue, it also depends on the type of specimen and its tissue density. It will always be a bit of a guess, but the above calculation surely helps in defining a minimum time per transfer bath.</div>
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Kind regards,</div>
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Dries</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>Van:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> namens Robyn Cumming <robyn.cumming@qm.qld.gov.au><br>
<b>Verzonden:</b> vrijdag 25 augustus 2023 04:04<br>
<b>Aan:</b> nhcoll-l@mailman.yale.edu <nhcoll-l@mailman.yale.edu><br>
<b>Onderwerp:</b> [Nhcoll-l] Increasing ethanol concentration in stages</font>
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<p class="x_MsoNormal">Hi all</p>
<p class="x_MsoNormal">I have some wet specimens with ethanol concentration below what is desirable (50-60%) and am topping them up in stages of ~5% back up to 70% to avoid osmotic shock.</p>
<p class="x_MsoNormal"> </p>
<p class="x_MsoNormal">My question: does anybody know how long I should wait between stages? Is a few days long enough to allow the specimens to adjust before increasing the concentration again?</p>
<p class="x_MsoNormal"> </p>
<p class="x_MsoNormal">I haven’t been able to find anything in the literature on this. Any advice greatly appreciated!</p>
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<p class="x_MsoNormal" style=""><b><span style="font-size:12.0pt; font-family:"Lucida Handwriting"; color:#500BB5">Best wishes, Robyn</span></b></p>
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