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Dear all: </div>
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Bioarchaeologist here...do you all consider tooth/bone or hair for potential isotope sampling? Those do preserve without special care, but just curious. We have a similar type of list for burials but face the challenge of asking for exports for future sampling
when we also are required to produce data within a set timeframe. </div>
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Carolyn</div>
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<div class="PlainText">Dr. Carolyn Freiwald<br>
Department of Sociology and Anthropology<br>
University of Mississippi<br>
544 Lamar<br>
University MS 38677<br>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> on behalf of Jonathan Dunnum <jldunnum@unm.edu><br>
<b>Sent:</b> Tuesday, October 17, 2023 10:47 AM<br>
<b>To:</b> Mullon, Madeleine <madeleine_mullon@fas.harvard.edu>; Hawkins, Rebecca K. <rkhawkins@ou.edu>; nhcoll-l@mailman.yale.edu <nhcoll-l@mailman.yale.edu><br>
<b>Subject:</b> Re: [Nhcoll-l] Best tissues to take for mammal specimens</font>
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<td>[EXTERNAL]</td>
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Hi All,</div>
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Cody beat me to it. I would suggest that as we move forward and grow collections into resources available to a much wider range of users (e.g., pathobiology, global health) that we as a community are not maximizing the breadth of sampling and utility of our
specimens. The gold standard is a true "holistic specimen". Below is an example of what we took on a recent trip. </div>
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<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:14.0pt; font-family:"Arial",sans-serif">Sample Collection - Panama Picante Project</span></p>
<p class="x_MsoNormal"><span style="font-size:6.5pt; font-family:"Arial",sans-serif"> </span></p>
<span style=""></span><span style=""></span><span style=""></span><span style=""></span><span style=""></span><span style=""></span><span style=""></span><span style=""></span><span style=""></span><span style=""></span><span style=""></span><span style=""></span>
<table class="x_MsoTableGrid x_ContentPasted0" style="border-collapse:collapse; border:none">
<tbody class="x_ContentPasted0">
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<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 2.25pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><b><span class="x_ContentPasted0" style="font-size:9.0pt; font-family:"Arial",sans-serif">part</span></b></p>
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<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 2.25pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><b><span class="x_ContentPasted0" style="font-size:9.0pt; font-family:"Arial",sans-serif">preservative</span></b></p>
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<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 2.25pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><b><span class="x_ContentPasted0" style="font-size:9.0pt; font-family:"Arial",sans-serif">container</span></b></p>
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<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 2.25pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><b><span class="x_ContentPasted0" style="font-size:9.0pt; font-family:"Arial",sans-serif">storage</span></b></p>
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<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Heart, lung</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
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<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Liquid nitrogen</span></p>
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<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Lung (subsample for screening in Japan)</span></p>
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<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">RNALater</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient for a day then</span></p>
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">liquid nitrogen or -80C</span></p>
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<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Whole lung (fixed for lung modeling)</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">formalin</span></p>
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<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Scintillation vial</span></p>
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<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient</span></p>
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<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Kidney, spleen</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal x_ContentPasted0">Liquid nitrogen<span style="font-size:9.0pt"></span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Liver</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal x_ContentPasted0">Liquid nitrogen<span style="font-size:9.0pt"></span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Tissue subsamples for Gorgas</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal x_ContentPasted0">Liquid nitrogen<span style="font-size:9.0pt"></span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Large intestine w/feces</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal x_ContentPasted0">Liquid nitrogen<span style="font-size:9.0pt"></span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Blood (dry)</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Nubuto</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">envelope</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient to -20C</span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><a class="x_ContentPasted0"><span style="font-size:9.0pt">Blood (smear)</span></a></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">Methanol</span></span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">slide</span></span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient to -20C</span></span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">Oral swab (viral)</span></span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">DNA/RNA shield</span></span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="">Liquid nitrogen</span><span style=""><span style="font-size:9.0pt"></span></span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">Anal swab (viral)</span></span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">DNA/RNA shield</span></span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span style=""><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="">Liquid nitrogen</span><span style=""><span style="font-size:9.0pt"></span></span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Wing swab (fungal)</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient to -20C</span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Oral swab (fungal)</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient to -20C</span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Anal swab (fungal)</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient to -20C</span></p>
</td>
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<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ectoparasites</span></p>
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<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">95% EtOH</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Cryovial or</span></p>
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">scintillation vial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient to -20C</span></p>
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<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Endoparasites</span></p>
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<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">70% EtOH</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">scintillation vial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient to -20C</span></p>
</td>
</tr>
<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Embryos (small)</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">none</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">cryovial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Liquid nitrogen</span></p>
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<tr class="x_ContentPasted0" style="">
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Embryos (large)</span></p>
</td>
<td width="114" valign="top" class="x_ContentPasted0" style="width:85.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">95% EtOH</span></p>
</td>
<td width="126" valign="top" class="x_ContentPasted0" style="width:94.5pt; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">20mL scintillation vial</span></p>
</td>
<td width="168" valign="top" class="x_ContentPasted0" style="width:1.75in; border:none; border-bottom:solid windowtext 1.0pt; padding:.05in 0in .05in 0in">
<p class="x_MsoNormal"><span class="x_ContentPasted0" style="font-size:9.0pt">Ambient to -20C</span></p>
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<p class="x_MsoNormal"><span style="font-family:"Times New Roman",serif"> </span></p>
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Jonathan L. Dunnum Ph.D. <span style="font-family:Tahoma,Geneva,sans-serif; text-align:start; background-color:rgb(255,255,255)">(he, him, his)</span><br>
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<div id="x_divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> on behalf of Mullon, Madeleine <madeleine_mullon@fas.harvard.edu><br>
<b>Sent:</b> Tuesday, October 17, 2023 9:27 AM<br>
<b>To:</b> Hawkins, Rebecca K. <rkhawkins@ou.edu>; nhcoll-l@mailman.yale.edu <nhcoll-l@mailman.yale.edu><br>
<b>Subject:</b> Re: [Nhcoll-l] Best tissues to take for mammal specimens</font>
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<p class="x_x_MsoNormal" style="line-height:12.0pt; background:#FF6666"><b><span style="font-size:10.0pt; color:white"> [EXTERNAL]</span></p>
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<p class="x_x_MsoNormal">Hi Rebecca,</p>
<p class="x_x_MsoNormal"> </p>
<p class="x_x_MsoNormal">If I recall correctly, the tissue found to have the greatest genomic DNA load was spleen. However, seeing as it’s impractical to locate the spleen on many small mammals, we opt for liver and muscle here at MCZ. Our current standard
practice is to take 1 vial of liver and 1 vial of skeletal muscle for storage in our cryogenic collection. Our thinking is that the liver is more rich in DNA yield, and the muscle is more stable in long-term cold storage. This works for most of our specimens,
which tend to be local species that are plentiful. If the specimen we are prepping is of particular interest or rarity, we will often take extra if the animal is large enough to do so. If the specimen is too decomposed, we may skip liver (since it autolyzes
so quickly) and instead take 2 muscle samples. We don’t typically save other tissue samples unless they have been specifically requested (heart tissue, skin samples in RNALater, etc.).</p>
<p class="x_x_MsoNormal"> </p>
<p class="x_x_MsoNormal">There was a good article out of Texas Tech about the cold preservation of different tissue types, and the time interval at which the genetic material degrades at different temperatures. Skeletal muscle is most stable over time, though
not as high in yield as spleen or liver, which both tend to autolyze quickly. Here:
<a href="http://dx.doi.org/10.1093/jmammal/gyab009" target="_blank">10.1093/jmammal/gyab009</a></p>
<p class="x_x_MsoNormal"> </p>
<p class="x_x_MsoNormal">This is a different paper I found while I was looking for the aforementioned one, more to do with preservation of RNA in liver and spleen: <a href="https://doi.org/10.1371%2Fjournal.pone.0167391" target="_blank">10.1371/journal.pone.0167391</a></p>
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<p class="x_x_MsoNormal">Best,</p>
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<p class="x_x_MsoNormal">Madeleine Mullon</p>
<p class="x_x_MsoNormal">She/they</p>
<p class="x_x_MsoNormal">MCZ Mammalogy</p>
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<p class="x_x_MsoNormal"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu>
<b>On Behalf Of </b>Hawkins, Rebecca K.<br>
<b>Sent:</b> Tuesday, October 17, 2023 9:42 AM<br>
<b>To:</b> nhcoll-l@mailman.yale.edu<br>
<b>Subject:</b> [Nhcoll-l] Best tissues to take for mammal specimens</p>
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<p class="x_x_MsoNormal"><span style="font-size:12.0pt; color:black">Hello all,</span></p>
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<p class="x_x_MsoNormal"><span style="font-size:12.0pt; color:black">Does anyone know of primary literature or have advice on the most useful tissue samples to take when preparing mammal specimens? We are looking to reevaluate what tissue samples we currently
take and which tissues are stored together. We currently take three tissue tubes per specimen for storage in liquid nitrogen: 1) heart and kidney, 2) liver, and 3) muscle. Thank you.</span></p>
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