<div dir="ltr">Hi, Rebecca! My response would be to take them all. You only get one opportunity to sample that species from a particular locality and point in time. Of course, this assumes you have ample growth space in your tissue collection.<div><br></div><div>Take care,</div><div>Cody</div><div><br></div><div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr"><div dir="ltr">Cody W. Thompson, PhD<br></div></div><div dir="ltr"><div>Mammal Collections Manager</div><div>& Associate Research Scientist<br><div>University of M<font face="arial, helvetica, sans-serif" size="2">ichigan</font></div><div><font face="arial, helvetica, sans-serif" size="2">Museum of Zoology</font></div><div><div style="color:rgb(0,0,0)"><font face="arial, helvetica, sans-serif" size="2">3600 Varsity Drive</font></div><div style="color:rgb(0,0,0)"><font face="arial, helvetica, sans-serif" size="2">Ann Arbor, Michigan 48108</font></div></div><div><font face="arial, helvetica, sans-serif" size="2">Office: (734) 615-2810</font></div><div>Fa<font face="arial, helvetica, sans-serif" size="2">x: (734) 763-4080</font></div><div><font face="arial, helvetica, sans-serif" size="2">Email: <a href="mailto:cwthomp@umich.edu" target="_blank">cwthomp@umich.edu</a></font></div><div><font size="2"><font face="arial, helvetica, sans-serif">W</font><font face="arial, sans-serif">ebsite: <a href="http://codythompson.org" target="_blank">codythompson.org</a></font></font></div><div><br></div><div><a href="https://lsa.umich.edu/ummz/mammals/donate-to-the-mammal-division.html" target="_blank"><b>Please click here to support the UMMZ Mammal Division, its collections, and research mission!!!</b></a><br></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Tue, Oct 17, 2023 at 11:28 AM Mullon, Madeleine <<a href="mailto:madeleine_mullon@fas.harvard.edu">madeleine_mullon@fas.harvard.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg4830857136385115201">
<div lang="EN-US" style="overflow-wrap: break-word;">
<div class="m_4830857136385115201WordSection1">
<p class="MsoNormal">Hi Rebecca,<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">If I recall correctly, the tissue found to have the greatest genomic DNA load was spleen. However, seeing as it’s impractical to locate the spleen on many small mammals, we opt for liver and muscle here at MCZ. Our current standard practice
is to take 1 vial of liver and 1 vial of skeletal muscle for storage in our cryogenic collection. Our thinking is that the liver is more rich in DNA yield, and the muscle is more stable in long-term cold storage. This works for most of our specimens, which
tend to be local species that are plentiful. If the specimen we are prepping is of particular interest or rarity, we will often take extra if the animal is large enough to do so. If the specimen is too decomposed, we may skip liver (since it autolyzes so quickly)
and instead take 2 muscle samples. We don’t typically save other tissue samples unless they have been specifically requested (heart tissue, skin samples in RNALater, etc.).<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">There was a good article out of Texas Tech about the cold preservation of different tissue types, and the time interval at which the genetic material degrades at different temperatures. Skeletal muscle is most stable over time, though not
as high in yield as spleen or liver, which both tend to autolyze quickly. Here: <a href="http://dx.doi.org/10.1093/jmammal/gyab009" target="_blank">
10.1093/jmammal/gyab009</a><u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">This is a different paper I found while I was looking for the aforementioned one, more to do with preservation of RNA in liver and spleen: <a href="https://doi.org/10.1371%2Fjournal.pone.0167391" target="_blank">10.1371/journal.pone.0167391</a><u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal">Best,<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal">Madeleine Mullon<u></u><u></u></p>
<p class="MsoNormal">She/they<u></u><u></u></p>
<p class="MsoNormal">MCZ Mammalogy<u></u><u></u></p>
<p class="MsoNormal"><span style="color:black"><img border="0" width="150" height="44" style="width: 1.5625in; height: 0.4583in;" id="m_4830857136385115201_x0000_i1025" src="https://huctw.org/wp-content/uploads/2015/05/huctw_email_sig_2012_white.jpg?download=1"></span><u></u><u></u></p>
</div>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<div style="border-right:none;border-bottom:none;border-left:none;border-top:1pt solid rgb(225,225,225);padding:3pt 0in 0in">
<p class="MsoNormal"><b>From:</b> Nhcoll-l <<a href="mailto:nhcoll-l-bounces@mailman.yale.edu" target="_blank">nhcoll-l-bounces@mailman.yale.edu</a>> <b>
On Behalf Of </b>Hawkins, Rebecca K.<br>
<b>Sent:</b> Tuesday, October 17, 2023 9:42 AM<br>
<b>To:</b> <a href="mailto:nhcoll-l@mailman.yale.edu" target="_blank">nhcoll-l@mailman.yale.edu</a><br>
<b>Subject:</b> [Nhcoll-l] Best tissues to take for mammal specimens<u></u><u></u></p>
</div>
</div>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal"><span style="font-size:12pt;color:black">Hello all,<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12pt;color:black"><u></u> <u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12pt;color:black">Does anyone know of primary literature or have advice on the most useful tissue samples to take when preparing mammal specimens? We are looking to reevaluate what tissue samples we currently take
and which tissues are stored together. We currently take three tissue tubes per specimen for storage in liquid nitrogen: 1) heart and kidney, 2) liver, and 3) muscle. Thank you.<u></u><u></u></span></p>
</div>
<div>
<div>
<p class="MsoNormal"><span style="font-size:12pt;color:black"><u></u> <u></u></span></p>
</div>
<div id="m_4830857136385115201Signature">
<div>
<div>
<p class="MsoNormal"><span class="m_4830857136385115201contentpasted0"><span style="font-size:12pt;color:black">Rebecca Hawkins (she/her)</span></span><span style="font-size:12pt;color:black"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:12pt;color:black">Curatorial Associate<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span class="m_4830857136385115201contentpasted0"><span style="font-size:12pt;color:black">Sam Noble Museum</span></span><span style="font-size:12pt;color:black"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span class="m_4830857136385115201contentpasted0"><span style="font-size:12pt;color:black">2401 Chautauqua Ave.</span></span><span style="font-size:12pt;color:black"><u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><span class="m_4830857136385115201contentpasted0"><span style="font-size:12pt;color:black">Norman, OK 73072</span></span><span style="font-size:12pt;color:black"><u></u><u></u></span></p>
</div>
</div>
</div>
</div>
</div>
</div>
_______________________________________________<br>
Nhcoll-l mailing list<br>
<a href="mailto:Nhcoll-l@mailman.yale.edu" target="_blank">Nhcoll-l@mailman.yale.edu</a><br>
<a href="https://mailman.yale.edu/mailman/listinfo/nhcoll-l" rel="noreferrer" target="_blank">https://mailman.yale.edu/mailman/listinfo/nhcoll-l</a><br>
<br>
_______________________________________________<br>
NHCOLL-L is brought to you by the Society for the Preservation of<br>
Natural History Collections (SPNHC), an international society whose<br>
mission is to improve the preservation, conservation and management of<br>
natural history collections to ensure their continuing value to<br>
society. See <a href="http://www.spnhc.org" rel="noreferrer" target="_blank">http://www.spnhc.org</a> for membership information.<br>
Advertising on NH-COLL-L is inappropriate.<br>
</div></blockquote></div>