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<div>We use a method devised by a former graduste student (and longtime friend of the museum.)</div>
<div dir="ltr">If you order a certain kind of patent-lip shell vial between 1 and 2 drams... they often come with a
<a href="https://acmevial.com/products/plastic-stopper-ag-1/" rel="noreferrer noopener" tabindex="0">
solid stopper made of polypropylene</a>. </div>
<div dir="ltr">These stoppers can be easily poked through with an awl or a lab probe, and you can easily push the pin of a stub into it. This makes for a very stable stub holder that can be stored either upside down, right side up, or sideways, and with a big
enough drying chamber you could probably store them in the slotted containers that vials usually ship in. </div>
<div dir="ltr">When the stopper + stub is placed back in the vial, it creates an an air and watertight seal, just like using the vial for fluids would do, so my feeling is that these would be very appropriate for types. We put a label on the inside of the vial
like we would any other (one that wraps around the inside and faces out, so it doesn't touch the stub), and store many of these in glass chambers with desiccant (3L bail top jars filled with dessicant work great.)</div>
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<div dir="ltr">I have had mixed experience with the TedPella singletons Paul mentioned.</div>
<div dir="ltr">They are pricy and the plastic has a strong tendency to craze/crack if opened and shut too many times. They are not really airtight before that, either. I do like the multi-stub holders TedPella sells, however, for keeping sets of stubs organized,
especially for hand carried transportation. </div>
<div dir="ltr">Good luck!</div>
<div dir="ltr">-N</div>
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<span style="background-color:rgb(255, 255, 255);font-size:9pt;font-family:Arial, sans-serif;color:rgb(51, 51, 51);line-height:1.5">-<br>
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of Evolution, Ecology, and Organismal Biology, The Ohio State University<br>
Museum of Biological Diversity</span></div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu> on behalf of Callomon,Paul <prc44@drexel.edu><br>
<b>Sent:</b> Saturday, December 14, 2024 7:42:57 AM<br>
<b>To:</b> Menard, Katrina <katrina.menard@uconn.edu>; NHCOLL-L <nhcoll-l@mailman.yale.edu><br>
<b>Subject:</b> Re: [Nhcoll-l] Holotype specimens on SEM stubs</font>
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We use single-stub storage tubes for long-term storage of SEM type material, coated and uncoated: https: //www. tedpella. com/storage-boxes-bags_html/SEM-mount-storage-boxes. aspx#_16630 These are sufficiently airtight that their internal conditions</div>
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<p class="x_MsoNormal">We use single-stub storage tubes for long-term storage of SEM type material, coated and uncoated:</p>
<p class="x_MsoNormal"><a href="https://urldefense.com/v3/__https://www.tedpella.com/storage-boxes-bags_html/SEM-mount-storage-boxes.aspx*_16630__;Iw!!KGKeukY!0QtNjy_OiZxzYpGazsRxZwQPXOGIgsalWGoCzwCXEPz4Su74TaA8bsyK-ymPfgnvMdLPZhkxcNW0oio4$">https://www.tedpella.com/storage-boxes-bags_html/SEM-mount-storage-boxes.aspx#_16630</a></p>
<p class="x_MsoNormal">These are sufficiently airtight that their internal conditions will equilibriate, and we keep them in cabinets within our normal collection space, which is 50-65 degrees F and 50-70% rH.</p>
<p class="x_MsoNormal">Of course, this likely will prove too expensive for large-scale storage, in which case a dry chamber or dry room is likely to be more economical.</p>
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<p class="x_MsoNormal"><b><span style="font-size:10.0pt; font-family:"Tahoma",sans-serif; color:black">Paul Callomon</span></b><span style="font-size:10.0pt; font-family:"Tahoma",sans-serif; color:black"><br>
<i>Collection Manager, Malacology and General Invertebrates</i></span><span style=""></span></p>
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<p class="x_MsoNormal"><b><span style="font-size:10.0pt; font-family:"Verdana",sans-serif; color:black">Academy of Natural Sciences of Drexel University, Philadelphia</span></b><span style="font-size:10.0pt; font-family:"Tahoma",sans-serif; color:black"><br>
<i><a href="mailto:callomon@ansp.org"><span style="color:blue">callomon@ansp.org</span></a> Tel 215-405-5096 - Fax 215-299-1170</i></span><span style=""></span></p>
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<p class="x_MsoNormal" style="margin-left:.5in"><b><span style="font-size:11.0pt; font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt; font-family:"Calibri",sans-serif"> Nhcoll-l <nhcoll-l-bounces@mailman.yale.edu>
<b>On Behalf Of </b>Menard, Katrina<br>
<b>Sent:</b> Friday, December 13, 2024 12:32 PM<br>
<b>To:</b> NHCOLL-L <nhcoll-l@mailman.yale.edu><br>
<b>Subject:</b> [Nhcoll-l] Holotype specimens on SEM stubs</span></p>
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<p><b><span style="font-family:"Arial",sans-serif; font-variant:small-caps; color:black">External.</span></b><b><span style="font-family:"Arial",sans-serif; font-variant:small-caps"></span></b></p>
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<p class="x_MsoNormal" style="margin-left:.5in">Hi All, </p>
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<p class="x_MsoNormal" style="margin-left:.5in">We have received a request to deposit a holotype of a protist into our collection, but the specimen was used for SEM. We have desiccation chambers for storing SEM stubs for working research, but I wanted to know
if there is a best practice for long-term storage of SEM specimens that are designated holotypes/type material. Do other collections utilize sequestered chambers for type material, or is there another way to store unicellular type material that was coated?</p>
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<p class="x_MsoNormal" style="margin-left:.5in">Thank you for all of your input and suggestions,</p>
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<p class="x_MsoNormal" style="margin-left:.5in">Katrina</p>
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<p class="x_MsoNormal" style="margin-left:.5in"><span style="color:black">------------------------------------------------------------------------------<br>
Dr. Katrina Menard (she/her)<br>
Collection Manager, Invertebrates<br>
Ecology & Evolutionary Biology<br>
University of Connecticut<br>
Unit 3043, 75 N. Eagleville Rd.<br>
Storrs, CT 06269-3043<br>
email: <a href="mailto:katrina.menard@uconn.edu">katrina.menard@uconn.edu</a><br>
<br>
Zootaxa Section Editor: Miroidea</span></p>
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