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Hi everyone, </div>
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With apologies for cross-posting, I'm looking for some advice about working with a damaged specimen. </div>
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It's a newborn monkey that was originally an alizarin transparency, prepared by the Spalteholz technique (<a class="OWAAutoLink" id="OWAf6042ca3-eb70-cd33-aa2e-734706ad1500" href="https://doi.org/10.1016/s0940-9602(01)80020-0">https://doi.org/10.1016/s0940-9602(01)80020-0</a>),
with our catalogue listing the preservative as "Methyl salicylate 5: benzyl benzoate 4".</div>
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In 2008 it was apparently in poor condition with crystal deposition, and it was transferred into 100% glycerol. </div>
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Currently, a lipid layer has formed at the top of the fluid, which is also cloudy - presumably also with suspended lipids. </div>
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The tissue is no longer transparent. </div>
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My questions are: </div>
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Is it possible to make the tissues transparent again? (If so, how?!)</div>
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Is it best to keep the specimen in glycerol, but dilute it to a more appropriate concentration? </div>
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<span style="color: rgb(0, 0, 0);">Many thanks,</span><br>
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Claire</p>
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<span style="color: rgb(50, 49, 48);"> </span></p>
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<span style="color: rgb(50, 49, 48);">*******</span></p>
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<span style="color: rgb(50, 49, 48);"><b>Survey: Fluid Preservation Methods in Biological Collections</b></span></p>
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<span style="color: blue;"><u><a style="color: blue; margin-top: 0px; margin-bottom: 0px;" href="https://app.onlinesurveys.jisc.ac.uk/s/reading/fluid-preservation-methods-in-biological-collections">https://app.onlinesurveys.jisc.ac.uk/s/reading/fluid-preservation-methods-in-biological-collections</a></u></span></p>
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<span style="color: rgb(50, 49, 48);"> </span></p>
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<span style="color: rgb(50, 49, 48);">*******</span></p>
<p style="background-color: white; margin: 0cm;" class="elementToProof"><span style="font-family: Calibri, sans-serif; font-size: 11pt; color: rgb(50, 49, 48);"><b>Claire Smith
</b>(she/her), AFHEA </span></p>
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<span style="color: rgb(50, 49, 48);"><b>Graduate Teaching Assistant: </b>Tuesdays and Wednesdays</span></p>
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<span style="color: rgb(50, 49, 48);"><b>PhD Researcher: </b>Mondays, Thursdays, and Fridays</span></p>
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</b></span></p>
<p style="background-color: white; margin: 0cm;" class="elementToProof"><span style="font-family: Calibri, sans-serif; font-size: 14.6667px; color: rgb(50, 49, 48);"><b>Cole Museum of Zoology</b></span></p>
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<span style="color: rgb(50, 49, 48);">University of Reading</span></p>
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<span style="color: rgb(50, 49, 48);"><br>
</span><span style="color: blue;"><u><a style="color: blue; margin-top: 0px; margin-bottom: 0px;" target="_blank" href="mailto:claire.smith@reading.ac.uk">claire.smith@reading.ac.uk</a></u></span><span style="color: rgb(0, 112, 192);">
</span></p>
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<span style="color: blue; background-color: white;"><u><a style="color: blue; margin-top: 0px; margin-bottom: 0px;" href="http://www.linkedin.com/in/wetconservatrix">www.linkedin.com/in/wetconservatrix</a></u></span><span style="color: rgb(0, 112, 192);"><br>
</span><span style="color: black;">Social media: @wetconservatrix</span></p>
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