NEED PARNASSIUS BEHRII AND P. SACERDOS/suggestion

[Clark Thompson]

Suggestion:

Place a want ad under 'Lepidoptera' in The Insect Classifieds:
http://www.insectnet.com/cgi/classifieds/classifieds.cgi

and in the InsectNet.com Forum under 'Lepidoptera'
http://www.insectnet.com/cgi/dcforum/dcboard.cgi

Both venues are free of charge. Lots of traffic. You might get a bite.

- Clark

-----Original Message-----
From: owner-leps-l at lists.yale.edu [mailto:owner-leps-l at lists.yale.edu]On
Behalf Of Evgueni Zakharov
Sent: Tuesday, December 03, 2002 10:57 AM
To: leps-l at lists.yale.edu; TILS-leps-talk at yahoogroups.com
Subject: NEED PARNASSIUS BEHRII AND P. SACERDOS


Does anyone have a few Parnassius behrii and P. sacerdos to spare for DNA
research?


I am a postdoc working with Felix Sperling at the University of Alberta. I
have been doing DNA-based research on the phylogeny and geographic variation
of  the Parnassius phoebus-smintheus complex. I AM trying to determine
taxonomic boundaries of the group across the Holarctic.

I need just a few papered specimens that have been kept dried and unrelaxed,
preferably, less than 2-3 years after collecting. Material up to about 20
years old would be welcome too; it just has a declining probability of
giving
useable DNA. If someone, by any chance, has alcohol preserved material, this
would be even better.

According to our DNA sequence data, P. phoebus and P. smintheus are well
diverged. P. bremeri, which is usually treated as an independent allopatric
species from
P. phoebus species in Siberia, shows no or little difference from P. phoebus
from Eurasia, Alaska
and the central Yukon. I have sequenced 825bp of COI for almost 100
specimens
from Eurasia and
North America but to complete the picture we really a few more specimens of
Parnassius sacerdos (from the European Alps) and Parnassius behrii > >(from
the Sierra Nevada, California).


So, if anyone hase some of these specimens to spare (or even a couple of
their legs and a digital scan of the rest of the specimen) I would be very
grateful for your help.

Many thanks in advance,
Evgueni

Evgueni Zakharov
Ph.D. Postdoctoral Researcher
Department of Biological Sciences
University of Alberta
Edmonton, Alberta T6G 2E9
CANADA

Tel.: (780) 492-0587
Fax: (780) 492-9234



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