[NHCOLL-L:240] Accessioning and DNA sample query

[ANDREW BENTLEY]

Hi all

I wonder if any of you could help me with a query regarding 
accessioning of specimens and DNA samples.

Firstly, we are wondering what to do with lots accessioned into our 
fish collection which get split after arriving.  For instance, a lot of 15 
fish come in to be accessioned.  However, 5 of the fish are cleared 
and stained while 5 are turned into dry specimens while the other 
five remain in the wet collection.  In your collections would these 
three sublots retain the same accession or catalog number or 
would they be given discreet numbers??  Are they in some way 
cross referenced in your database?  I would be interested to hear 
from anyone who has ideas as we are prsently considering 
converting to a new database that can handle separate database 
fiiles for the various parts of our collection i.e. wet, dry, DNA, C&S, 
X-ray, image etc. and theoretically one could have the same 
number for a lot, parts of which belong to each 'sub-collection' just 
with a different prefix or identifying character.

2.  We are in the process of getting a DNA collection started and I 
was wondering if anyone could help with some policy on the 
loaning of such specimens.  Essentially a DNA specimen cannto 
be loaned - it is a gift of material which will not be returned??  What 
prerequisites have been set by other organisations in terms of 
lodging DNA sequences or data with the loaning organisation or a 
recognised gene bank in return for the use of material??

3.  What concentration of ethanol is best for long term storage of 
DNA specimen material if no cryogenic facilities are available??

Any ideas etc would be greatly appreciated

Thanking you all in advance

Andy


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Ý    ---------------        ANDY BENTLEY          ---------------    Ý
Ý    -----------         COLLECTION MANAGER           -----------    Ý
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