[NHCOLL-L:1392] RE: Sealing bones after DNA sampling.

[Julian.Carter at nmgw.ac.uk]

A good query! A possible suggestion would be to use a stable acrylic polymer
such as Paraloid / acryloid B72 (about 20% w/v in acetone) mixed in with
glass microballoons. This makes a good reversible filler that should not
affect the surrounding bone structure, or become too deeply absorbed that it
would not alter future biochemical analysis, and is an obvious repair.
Jules Carter
Conservation Officer
Natioal Museums and Galleries of Wales

-----Original Message-----
From: Paul Scofield [mailto:pscofield at cantmus.govt.nz]
Sent: 13 December 2001 03:34
To: 'NHCOLL-L at LISTS.YALE.EDU'
Subject: [NHCOLL-L:1391] Sealing bones after DNA sampling.


We have recently have had various researchers sampling subfossil bird bones
in our collection for DNA using dremels and narrow gauge corers. This
generally leaves the marrow in the center of the bone exposed directly to
the air for the first time since the death of the bird. presumably it may be
important to seal up the hole to prevent contamination, excessive drying and
further DNA deterioration. I do feel that it is unnecessary to attempt to
make the bone look as good as new - this not only creates problems in
identifying what is natural and what is recreated but also gives a false
impression of the destructive nature of the sampling. Let be honest DNA
sampling -is destructive! 

Does anyone have any suggestions on sound methods for sealing the bone after
sampling that don't detract too much from the aesthetic and morphometrically
important aspects of the bones.

Regards Paul Scofield

Collections Manager - Natural History
Canterbury Museum,
Rolleston Avenue,
Christchurch, 8001
New Zealand.
Ph 64-3-3669429 ext 853 (direct)
Ph 64-3-366 5000 (switchboard)

Fax 64-3-366 5622






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