[NHCOLL-L:4363] how to dissolve and puffer paraformaldehyde in the field?
Dirk Neumann
Dirk.Neumann at zsm.mwn.de
Wed Jun 24 02:59:11 EDT 2009
Hi all,
for our next field trip to Africa we are considering to fixate specimens
with puffered paraformaldehyde solution; to carry cristalline
paraformaldehyde is onboard of aircraft is 100 % conform with IATA
regulations and thus avoiding difficulties of liquid formalin solutions
(to carry solutions with concentrations < / = 10 % is uneffecetive in
terms of fixations effort per carried quantity).
For puffering, the "Fish Collection Methods and Standards" recommend to
puffer 16g paraformaldehyde with 4 g anhydrous sodium carbonate and
dissolve in 400 ml distilled water to have a 10 % buffered formalin
solution (refers to McAllister, 1965).
Do you have experiences with this method or are there any other methods
to solve and puffer paraformaldehyde ?
Thanks for your comments & help
Dirk
--
Dirk Neumann
Tel: 089 / 8107-111
Fax: 089 / 8107-300
email: Dirk.Neumann(a)zsm.mwn.de
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Staatliche Naturwissenschaftliche Sammlungen Bayerns
Zoologische Staatssammlung München
Dirk Neumann, Sektion Ichthyologie / DNA-Labor
Münchhausenstr. 21
81247 München
Besuchen Sie unsere Sammlung:
http://www.zsm.mwn.de/ich/
---------
Dirk Neumann
Tel: +49-89-8107-111
Fax: +49-89-8107-300
email: Dirk.Neumann(a)zsm.mwn.de
postal address:
Bavarian Natural History Collections
The Bavarian State Collection of Zoology
Dirk Neumann, Section Ichthyology / DNA-Lab
Muenchhausenstr. 21
81247 Munich (Germany)
Visit our section at:
http://www.zsm.mwn.de/ich/
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