[Nhcoll-l] Fwd: Preservation for SEM

[Edwin Cruz-Rivera]

Hello all,
  First time poster here.  Someone in the crust-L list suggested that I
send my query to the experts in this list.  I apologize for cross-posting.
Here it is:

  What is the best preservation medium for crustaceans in order to do SEM
(and possibly TEM)?  I have a number of old samples that are preserved in
10% formalin/seawater and I wonder if those are suitable for looking at
external and internal (e.g., gut) fine structure.  I do not know the
history of the samples (buffered or not, any other preservatives) because
they were "inherited."  If recollection is needed because these are no
good, what then should be the right preservation protocol?
Your thoughts are appreciated,
Edwin
-- 
Dr. Edwin Cruz-Rivera
Assistant Professor of Marine Biology
Department of Biology
American University in Cairo
AUC Avenue, P.O. Box 74
New Cairo 11835, Egypt

Tel. 20.2.2615.2906
Fax 20.2.2795.7565
Email: edwincruz at aucegypt.edu

"It is not the same to hear the devil as to see him coming your way"
(Puerto Rican proverb)
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