[Nhcoll-l] rehydrating fish (was Rehydrating crustaceans)
Alastair.Graham at csiro.au
Alastair.Graham at csiro.au
Thu Jun 25 22:16:56 EDT 2015
Hello everyone
I have never heard of "Aerosol OT". Has anyone used this product on dried (formalin-fixed and then ethanol-preserved) fish?
I have been told that trisodium phosphate solution is recommended. However, the results are often unsatisfactory and perhaps it best to just leave the specimen dry.
Cheers
Al
Alastair (Al) Graham
Fish Collection Manager
Australian National Fish Collection
National Research Collections Australia
CSIRO National Facilities and Collections
P: +61 3 6232 5351 | M: +61 (0) 419 756 411 | F: +61 3 6232 5000
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From: nhcoll-l-bounces at mailman.yale.edu [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Yemisi Dare
Sent: Friday, 26 June 2015 3:07 AM
To: Simon Moore; Lazo-Wasem, Eric
Cc: Smith, Jamie; nhcoll-l at mailman.yale.edu
Subject: Re: [Nhcoll-l] Rehydrating crustaceans
Thank you very much Eric and Simon, for these pointers!
Yemisi.
From: Simon Moore [mailto:couteaufin at btinternet.com]
Sent: June-25-15 12:43 PM
To: Lazo-Wasem, Eric
Cc: Yemisi Dare; Smith, Jamie; nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>
Subject: Re: [Nhcoll-l] Rehydrating crustaceans
The OT spray is Na di-octyl sulpho-succinate - now in a convenient spray, should be good and many thanks for the update.
With all good wishes, Simon.
Simon Moore MIScT, RSci, FLS, ACR
Conservator of Natural Sciences and Cutlery Historian,
www.natural-history-conservation.com<https://urldefense.proofpoint.com/v2/url?u=http-3A__www.natural-2Dhistory-2Dconservation.com&d=AwMFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=ukpmynGv5iF5Bj88dqTmuJjC1bACxgWHbarbQguPbL4&s=7p5MoeHMlYRB7dlSJ0BxKLmt05qxTVQStqm56930HVQ&e=>
On 25 Jun 2015, at 16:53, Lazo-Wasem, Eric <eric.lazo-wasem at yale.edu<mailto:eric.lazo-wasem at yale.edu>> wrote:
Dear Yemesi,
I have generally placed the dried out material in a small container (capped vial, watch glass, etc.) along with a small volume of the wetting agent and let it sit overnight. I continue monitoring daily until the crustacean feels flexible and there are no more air bubbles visible inside the exoskeleton. I have actually forgotten samples and left them sit for more than a week, but in practice I think it usually takes 3-4 days for small things (amphipods, copepods) and a good week or so for something larger (such as the leeches I mentioned). The trick is to monitor regularly and when it appears "done" you can go back straight into ethanol (70-75% is what we use).
Eric LW
-----Original Message-----
From: Yemisi Dare [mailto:YDare at mus-nature.ca]
Sent: Thursday, June 25, 2015 11:43 AM
To: Lazo-Wasem, Eric; Smith, Jamie
Subject: RE: Rehydrating crustaceans
Dear Eric, Jamie,
We have precisely the same issue here and I have been looking for a resource somewhere on how to rehydrate some of our crustacean donations that are severely dessicated.
Eric do you have a written protocol that I can use?
Thank you very much,
Yemisi Dare.
Yemisi Dare, Ph.D.
Assistant Collections Manager, Invertebrates / Gestionnaire adjointe des collections invertébrés Canadian Museum of Nature / Musée canadien de la Nature PO Box 3443 Station D / CP 3443 Succ <<D>> Ottawa, ON Canada K1P 6P4
Tel: 613-566 4736/ F: 613-364-4027
E-Mail: ydare at mus-nature.ca<mailto:ydare at mus-nature.ca>
-----Original Message-----
From: nhcoll-l-bounces at mailman.yale.edu<mailto:nhcoll-l-bounces at mailman.yale.edu> [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Lazo-Wasem, Eric
Sent: June-25-15 11:30 AM
To: Smith, Jamie
Cc: nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>
Subject: Re: [Nhcoll-l] Rehydrating crustaceans
Dear Jamie,
I have been using "Aerosol OT," a wetting agent suggested to me by Bill Moser at the NMNH. We have successfully been rehydrating tough as leather dried out leeches (140 years old) and amphipod crustaceans (types dried out from 1871). The latter rehydrate so well that I can dissect the specimens and make permanent stained mounts in balsam. Even plankton sample more than 50 years old have been rehydrated, and we can successfully mount copepods and daphniids after a weeks soak in A-OT.
An alternative is Tri-basic calcium phosphate, or the old house painters washing solution, TSP (tri-sodium phosphate). Neither of these work nearly as well as Aerosol OT.
Eric Lazo-Wasem
Yale Peabody Museum
-----Original Message-----
From: nhcoll-l-bounces at mailman.yale.edu<mailto:nhcoll-l-bounces at mailman.yale.edu> [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Smith, Jamie
Sent: Thursday, June 25, 2015 11:04 AM
To: nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>
Subject: [Nhcoll-l] Rehydrating crustaceans
Has anyone had any luck rehydrating crustaceans that have completely dried out? If so, which method did you use?
Jamie M. Smith
Collections Manager, Invertebrates
North Carolina State Museum of Natural Sciences
Phone: (919)707-8869
Fax: (919)715-2294
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