[Nhcoll-l] Polycarbonate cryogenic box manufacture

Fri May 29 10:06:07 EDT 2015

Heath

We use the following combination of polycarbonate boxes and 2ml cryovials from Fisher and they work just great.  81 tubes fit into the boxes with room to spare and no crowding of tubes of scratching off of labels.

https://urldefense.proofpoint.com/v2/url?u=https-3A__www.fishersci.com_shop_products_thermo-2Dscientific-2Dcryoboxes-2Dcryoboxes-2Dpc-2Dwhite-2Dholds-2D81-2Dvials-2D133-2Dx-2D133-2Dx-2D52-2Dmm_15350107b-3Fkrypto-3DJJxJGZ1BqepBumi6p5LV1sKC4ZvWJ9Dk-26ddkey-3Dhttp-3ASearch&d=AwIFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=FyvTaW_OvZ_sHvUdAqHfMcSW9eX0bg4N-Ym7AFI-c3g&s=jkKPoOWcDMIf5g6GRXCl1ySSYsuDj7lJPzcKYPkyoWQ&e= 

https://urldefense.proofpoint.com/v2/url?u=https-3A__www.fishersci.com_shop_products_thermo-2Dscientific-2Dnalgene-2Dgeneral-2Dlong-2Dterm-2Dstorage-2Dcryogenic-2Dtubes-2Dnalgene-2Dcryogenic-2Dtube-2Dsterile-2Dbulk-2Dpacked-2D2-2D0ml-2Dext-2Dthread-2Dconical-2Dbottom_033377w-3Fkrypto-3DJJxJGZ1BqeprY9HWHSFHvkCyYLyYcPEb-26ddkey-3Dhttp-3ASearch&d=AwIFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=FyvTaW_OvZ_sHvUdAqHfMcSW9eX0bg4N-Ym7AFI-c3g&s=7eTLovgcpAO7slbo4aNyVeBB9fbgUBi5uKpnUcHp5cc&e= 

Hope that helps

Andy

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Andy Bentley
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From: nhcoll-l-bounces at mailman.yale.edu [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Dirk Neumann
Sent: Friday, May 29, 2015 1:53 AM
To: nhcoll-l at mailman.yale.edu
Subject: Re: [Nhcoll-l] Polycarbonate cryogenic box manufacture

Hi Heath,

yes, isn't it funny that there is a huge variety of external-threated tubes and hardly a rack that allows proper storage and easy picking of single tubes ? Boxes with rigid internal grids don't work and scratch labels off as you explained; maybe its worth to check these ones:

Greiner (sorry, German text but PDF offers 2 detail views of boxes); US contact: Greiner Bio-One North America Inc., info at us.gbo.com<mailto:info at us.gbo.com>

Fisher Scientific offers similar boxes, acc. to their catalogue from

Wheaton, article 1267-3395 (100 tubes) / 1266-3405 (81 tubes)
Corning, article 1065-9351 (100 tubes) / 1039-0502 (81 tubes)
https://urldefense.proofpoint.com/v2/url?u=https-3A__de.fishersci.com_deen_-3Foption-3Dcom-5Finsight2-26task-3Dgetproduct-26productCode-3D10390502&d=AwIFAw&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=FyvTaW_OvZ_sHvUdAqHfMcSW9eX0bg4N-Ym7AFI-c3g&s=FntIp8GBd9U1Rn5z8XT9zbTC6sr8n87lanEoH19u05s&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__de.fishersci.com_deen_-3Foption-3Dcom-5Finsight2-26task-3Dgetproduct-26productCode-3D10390502&d=AwMD-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=y5p_rqm3rsvlr94cxpWf8qwGDQrn-6H8KmcNy6E7GYY&s=5fCXC88vCF0l1i2J_mAFLGzM3blc6VvteOBnSfbcNrM&e=>

For some internal reasons, the article numbers from the printed catalogue do not match with the online catalogue, and also the pictures differ. Both alternatives have internal posts that hold the tubes, not a grid. Even with those racks spacing of tubes might be an issue, as external dimensions of boxes are designed to fit in freezer racks which do have fixed dimensions. We have been testing similar boxes with the same design, tubes fit inside fairly without rubbing external labels, but we decided to use tall NUNC storage vials, as they perform much better during field, lab & under frozen (-25°C) conditions (externally threaded tubes do have major evaporation issues under ambient temperatures and under field conditions).

Hope this helps
Dirk

Am 29.05.2015 um 00:42 schrieb Garner, Heath:
Hi,

We're needing to switch the storage of our frozen tissue collection into 81-cell polycarbonate boxes. Unfortunately, about 95% of our frozen collection are in externally threaded tubes with labels, which make the vials themselves too wide to be placed in your typical cryogenic storage box. The labels tear and scuff on the edges of the divider or the vial caps become so closely impacted that the lids to the box no longer fit. We've tried every popular brand of polycarbonate cryogenic storage box on the market that's readily available in the U.S. and certainly no grid-style cryo box fits our tubes. Most appear to be the exact same or similar design, just with a different distributers logo printed on them.

Does anyone have positive experience with a laboratory or plastics company that's willing to manufacture something for you, provided you pay for the mold costs and order suitable quantities? So far we're not having much luck. Feel free to contact off-list.

Thanks!
Heath

---------------------------------------------
Heath J. Garner
Curator of Collections
Museum of Texas Tech University
Natural Science Research Laboratory
3301 4th Street
Lubbock, TX 79415
(806) 742-2486

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