[Nhcoll-l] Clearing & Staining issue

[Sebastien Enault]

Hello all,

I have a quick question for those of you that routinely use clearing and
staining for embryonic specimens. I mostly use the technique on small fish
and herps without issues, but when processing foetal/juvenile rodents, I've
had white tissue not clearing at all during the process. Attached are a
couple of pictures to illustrate the issue. This happened on two distinct
batches, and if any of you know what's going on and how to solve the issue,
that would be greatly appreciated. So far the only way it could be removed
is by careful manual scraping, which is obviously not satisfactory
considering how time consuming it is and due to the increased risk to
damage such fragile specimens.
Thanks and best regards,
S. Enault
======================
Sebastien Enault, PhD
www.kraniata.com
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