[Nhcoll-l] USDA treatments

[Charles M. Dardia]

We have the opportunity to receive skins, tissues and skeletons from 3 species hedgehogs originating in Israel.  The material must be treated due to the presence of foot and mouth disease in the region but all the treatments seem like a detriment to the skins and the tissues. I have pasted below treatments the USDA will accept. Does anyone have any experience with these methods as it relates to study skins and tissue intended for molecular use?


Charles

*         A heat treatment at a minimum of 72 degrees C for a minimum of 30 minutes exposure time; OR

*         A pH of 5.5 or less for a minimum of 30 minutes exposure time; OR

*         A pH of 10 or greater for a minimum of 2 hours exposure time; OR

*         Immersion in a minimum of 0.4 percent beta propriolactone/12 hours/4 degrees C/pH 7; OR

*         Immersion in a minimum of 0.2 percent glutaraldehyde; OR

*         Immersion in a minimum of 10 percent formalin


Charles M. Dardia
Collections Manager
Cornell Museum of Vertebrates
159 Sapsucker Woods Road
Ithaca, NY 14850
(607) 254-2161
http://cumv.cornell.edu/

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