[Nhcoll-l] Allozyme homogenates at your institution

[Wynn, Addison]

Dear colleagues,

We have had very promising results using a target-capture based approach to sequence DNA from the frozen tissue pellets and supernatants of samples originally prepared for studies of allozyme variation many decades ago. This investigation was inspired by the large quantity of such materials that are part of Richard Highton's important collection of plethodontid salamanders, now housed at the USNM. We intend to publish a short methods note alerting the community to utility of this material that our institutions have been safeguarding for decades. In this same publication, we would also like to alert readers to the availability of equivalent material in other institutions given that it may not be easy to find this information using standard collections searches. For this we need your help! We envision a summary table listing the institution, collection, approximate quantity of homogenates available, and brief notes  (e.g., USNM; Amphibians + Reptiles; 30,000; primarily North American salamanders). We can also include a mention of collections that do not have such material to (hopefully) reduce the number of unnecessary inquiries you may receive. Thank you for your consideration in taking part in this survey (message and form<https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.google.com%2Fforms%2Fd%2Fe%2F1FAIpQLSfxwyN9w8ntRkZ9rpHozvt7AVrfYmCBtSjk6t4B9L5q37RiEg%2Fviewform%3Fusp%3Dsf_link&data=04%7C01%7CWYNNA%40si.edu%7Ce6ad1213b0cd4dfd48e708d87b6f3a96%7C989b5e2a14e44efe93b78cdd5fc5d11c%7C0%7C0%7C637395064951750669%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=QkiOGOjhS0DGMKqZfXNcG7ELwCAIkKtgPXLTJVYGXkQ%3D&reserved=0>), and we appreciate any efforts to circulate this with your colleagues!

Rayna Bell (California Academy of Sciences)
Kyle O'Connell, Addison Wynn, Kevin Mulder, and Kevin de Queiroz (National Museum of Natural History, Smithsonian Institution)


Addison Wynn
Museum Specialist
Division of Amphibians & Reptiles

ph: 202-633-0737; wynna at si.edu<mailto:wynna at si.edu>

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY
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