[Nhcoll-l] Transporting flash frozen specimens [SEC=OFFICIAL]

[Haff, Tonya (NCMI, Black Mountain)]

OFFICIAL

Hello all,

We have an old liquid nitrogen dewar, which holds maybe 35L of LN2 (this is a guess I must admit, but it's pretty big). It is the kind that you just have to drop your tissue tubes into and then have to fish them out later.  I'm guessing it must be about 30 years old but it works pretty well at least in terms of holding temperature, and if properly cooled down can last up to about 3 or 4 weeks in the field if the temperatures are cool to mild. This is not too shabby - however we do a lot of work in the tropics, and our trips can be longer than 4 weeks in remote locations.

I am interested in whether any of you know of modern LN2 dewars that might last longer than this, or have some information on reliable dry shippers rather than dewars - or perhaps you combine the two to keep your flash frozen material at temperature for longer in the field? My primary concern is how long we can be in the field without having to worry that the dewar/dry shipper will run dry or warm up. My secondary interest is that it would be great to have something that makes it a bit easier to remove tissues once they're placed into the dewar. And I'd love to know if any of you use dry shippers that last as long as this dewar.

Thanks so much!

Cheers,

Tonya


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Dr Tonya Haff
Senior Collection Manager
Australian National Wildlife Collection
National Research Collections Australia, CSIRO
Canberra, ACT 2602 Australia
+61(0)419569109


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