[Nhcoll-l] Rehydration question

[Singer,Randal Anthony]

Tom,

I will refer you to a technique I have had great success with for amphibians, fishes and mammals developed by John Simmons. It involves placing the specimen inside a well sealed container (preferably a glass jar) on a mesh (rubber or metal) platform above, but not immersed in 100% deionized water. Warm water works best and a vapor should form inside the jar. Once the specimens looks sufficiently "wet" you transfer it to and immerse it in, a jar of 100% deionized water. As the water becomes cloudy, change it in increasing proportions of ethanol/isopropanol by 20% (0%, 20%, 40%...etc.) until you reach your desired preservative strength. I have had great success with this technique with vertebrates, but I have yet to try on invertebrates. I would be interested to see how it works.

Let me know if you have any further questions on the matter.

Cheers,

Randy Singer
________________________________
From: nhcoll-l-bounces at mailman.yale.edu [nhcoll-l-bounces at mailman.yale.edu] on behalf of Tom Schioette [TSchioette at snm.ku.dk]
Sent: Wednesday, July 24, 2013 4:58 AM
To: nhcoll-l at mailman.yale.edu
Subject: Re: [Nhcoll-l] Rehydration question

A former colleague of mine used Dioctyl Sodium Succinate on various marine invertebrates and was very happy with the results; I tried using it on molluscs after his recommendation and had moderate success, that is, it didn’t seem to work if the specimen was completely dried out and hard as a rock, but if there was a bit of moisture left in it, it could work rather well. A necessary part of the process was to put the specimen, while immersed in the solution, in a desiccator and turn it on, in order to get air bubbles out and pull as much of the liquid into existing cavities.

Cheers Tom


Tom Schiøtte
Natural History Museum of Denmark (Zoology)
Universitetsparken 15
DK-2100 Copenhagen OE
Denmark

TSchioette at snm.ku.dk



Fra: nhcoll-l-bounces at mailman.yale.edu [mailto:nhcoll-l-bounces at mailman.yale.edu] På vegne af Mandy Reid
Sendt: 24. juli 2013 01:18
Til: 'nhcoll-l at mailman.yale.edu'
Emne: [Nhcoll-l] Rehydration question

Does anyone have a method to rehydrate mollusc specimens that have dried in jars? I am wondering whether to try glycerine and am not sure whether to dilute it or use it neat.
Thanks
Mandy

Dr Mandy Reid
Collection Manager, Malacology
Australian Museum 6 College Street Sydney NSW 2010 Australia
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