[Nhcoll-l] Freezer storage of EtoH tissue samples

Sun Feb 1 16:38:22 EST 2015

Hi John,

This may not apply to you, but in Australia both total volume and the volume contained in individual storage containers is used to determine whether an EtOH collection (whether frozen or room temperature) requires specialised housing.

For example, if you have a total of 52 litres per freezer in a SINGLE container then it would merit requiring a flammable material freezer. However, if your total of 52 L is comprised of thousands of tiny vials then the risk is much lower. This is based on the premise that IF a fire started in that freezer, then each container would need to be breached for the EtOH to become hazardous.

We have an EtOH collection at room temperature that equates to many hundreds of litres of EtOH but have been advised that, because each vial is only a maximum of 10mL at most, that this does not require any special provision. Whether we should anyway is another question… :)

It might be worth checking if this rule also applies in your State/the US.

Cheers,
Beth

_____________________________________
Dr Beth Mantle
Research Operations Manager, National Research Collections Australia
CSIRO National Facilities and Collections
Please note that I work part-time hours: Monday – Wednesday.

P: +61 2 6246 4281 | M: +61 (0) 407 174 427 | F: +61 2 6246 5166
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-----nhcoll-l-bounces at mailman.yale.edu<mailto:-----nhcoll-l-bounces at mailman.yale.edu> wrote: -----
To: "nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>" <nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>>
From: "John P. Sullivan"
Sent by: nhcoll-l-bounces at mailman.yale.edu<mailto:nhcoll-l-bounces at mailman.yale.edu>
Date: 01/30/2015 12:26PM
Subject: [Nhcoll-l] Freezer storage of EtoH tissue samples

I am curious to know how people on this list deal with cold storage for DNA samples in ethanol.

We have thousands of tissue samples and fish fin clips preserved in 95% EtOH in 2ml Sarstedt screw cap vials. We typically store 16 boxes of 81 tubes per freezer rack and can fit 20 racks in a refrigerator/freezer.  This makes for a maximum of 52 liters or 13.7 gallons of EtOH stored in such a refrigerator/freezer.

We have been informed by Environmental Health and Safety at our institution that it is improper to store these in regular freezers (as opposed to prohibitively expensive, specialized “flammable material” freezers).

This person cited NFPA 45  10.5.2 and 12.2.2 and Article 501 of code 70 the National Electrical code.

Here are links to EH&S pages at several institutions that seem to indicate ethanol storage requires flammable material freezers:

https://urldefense.proofpoint.com/v2/url?u=http-3A__www.usd.edu_research_research-2Dand-2Dsponsored-2Dprograms_upload_Flammable-2DLiquids-2DStorage.pdf&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=xsAe-v5Z2ZE0fDchR_s4kuq9QC8BOgRyljnptUCARW4&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.usd.edu_research_research-2Dand-2Dsponsored-2Dprograms_upload_Flammable-2DLiquids-2DStorage.pdf&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=KF4QbWrSBgmNxBdy9sGpN5ZoayJnK3fFYHaiR9pdSK8&e=>
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ehs.washington.edu_fsofire_flamfrig.shtm&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=5etcgrUTAx-sFGPsHfiGxnvpQbT7G2HHgu8aWeEOzMM&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.ehs.washington.edu_fsofire_flamfrig.shtm&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=qdXV0xQBR7I0tPSImqpMt4pk8mwMg_bQcEQlWWOdHC4&e=>
https://urldefense.proofpoint.com/v2/url?u=http-3A__blink.ucsd.edu_safety_research-2Dlab_chemical_liquids_storage_index.html-23Determine-2Dallowable-2Dcontainer-2C-2D&d=AwIF-g&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=xtunBXc7eWM9AvSwGfsT0U45ZQZ2FVtjC4SoQP8zWww&s=eAeWqJhqyQ0DYcMDFwv9X9ZByrB15yqbTLRlrwbverI&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__blink.ucsd.edu_safety_research-2Dlab_chemical_liquids_storage_index.html-23Determine-2Dallowable-2Dcontainer-2C-2D&d=AwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=sfUtvnZ0J8NsTEIgQdXNNr7uxyEnp6bwuvR7T0JbELE&s=39ybek4t0u3PswjDfD_nsH9j4KPo9k7Ourm56R-91s8&e=>

However, my informal polling of colleagues on Facebook suggests that many of them do in fact store screw-top tubes with tissue samples in ethanol in regular freezers (though perhaps without the approval/oversight of their EH&S departments). My view is that fire/explosion risk from storage of these vials must be negligible to non-existent.  The flashpoint of pure EtoH is 16.6°C.  This temp would only be reached if a freezer failed and no action was taken for a long period of time. The risk of EtOH vapors concentrating in any quantity near internal mechanisms that could spark are minimal since tubes are sealed.

My questions are:  Has anyone here received explicit clearance from their EH&S to store EtOH tissue samples in regular freezers?  If you got this, what was required to obtain it?  If you have ethanol-preserved tissue samples, how do you store them?

This could make for an interesting and useful conversation, but feel free to contact me off-list if you’re concerned about being “on the record.”

- John Sullivan

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
John P. Sullivan, PhD

Collections Archivist & Curatorial Affiliate - Cornell University Museum of Vertebrates
Research Associate - The Academy of Natural Sciences of Drexel University

Cornell University Museum of Vertebrates
159 Sapsucker Woods Road
Ithaca, New York 14850-1923 USA

jpsullivan at cornell.edu<mailto:jpsullivan at cornell.edu> / office 607.254.6237, cell 607.342.2234, fax 607.254.2415

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