[Nhcoll-l] dna storage tubes for -80°C

[Bentley, Andrew Charles]

Dirk

We have been using these for a number of years now - https://urldefense.proofpoint.com/v2/url?u=https-3A__www.fishersci.com_shop_products_nalgene-2Dgeneral-2Dlong-2Dterm-2Dstorage-2Dcryogenic-2Dtubes_033377w-3FsearchHijack-3Dtrue-26searchTerm-3D033377W-26searchType-3DRAPID&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=uQg2Y4uc188QnChKc072r4at7J4xXMUw5btRbaRin8s&e= 

We specifically selected ungasketed, externally threaded tubes even for -80 freezers which we were using at the time.  There were too many reports of gasket failure at low temps and externally threaded tubes, although taking up slightly more space in storage, negate any contamination issues that may occur by having an internally threaded closure potentially in contact with tissue or preservation fluid.

Hope that helps

Andy

    A  :             A  :             A  :
 }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
    V                V                V
Andy Bentley
Ichthyology Collection Manager
University of Kansas
Biodiversity Institute
Dyche Hall
1345 Jayhawk Boulevard
Lawrence, KS, 66045-7561
USA

Tel: (785) 864-3863
Fax: (785) 864-5335
Email: abentley at ku.edu<mailto:abentley at ku.edu>
https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=-EM_--hP5W3AJYFlx2GF-QkRPBkkDIs8dFOIe_viTCU&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu_&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=5dr2Rc4jxMQRUmOyMCTo6XtwfwOmwn0Ue_jdOdYQq5U&e= >

SPNHC Past-President
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=VPrkG1m-GnoIg3BLUnAYe7BgUD4f9QaPHYp7ZINF-9Y&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.spnhc.org_&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=hQR_d-S8qVfe0uGPRSqZc1MKX2wqXO1vrGKihGZYYCI&e= >

                           :                 :
    A  :             A  :             A  :
 }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
    V                V                V

From: nhcoll-l-bounces at mailman.yale.edu [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Dirk Neumann
Sent: Thursday, December 01, 2016 9:03 AM
To: nhcoll-l at mailman.yale.edu
Subject: [Nhcoll-l] dna storage tubes for -80°C


Hi all,

I would like to know if there are any specific experiences (good and bad) on DNA-storage tubes (normal freezing as we don't have liquid-N storage).

We are using Matrix racks (sepra seals) and I want to change to high quality skrew-lid tubes, but I am sceptical with the silicone gasket in combination with the internal thread of the tubes.

Thermo says that the tubes are tight sealing (from liquid-N to normal freezing and even room temperature), but if the gasket starts to crumble, then the tubes necks are open. Also, gaskets are made of silicone, which might cause issues with cold-flowing behaviour and life span of the material.

Curious to hear your thoughts, especially if you have experiences where things turned out to be not that good as originally promised by the manufacturer (also off-line, if you wouldn't like to post any failures to the broad public)



Thanks in advance for any helpful feedback

Dirk



--

Dirk Neumann



Tel: 089 / 8107-111

Fax: 089 / 8107-300

email: Dirk.Neumann(a)zsm.mwn.de



Postanschrift:



Staatliche Naturwissenschaftliche Sammlungen Bayerns

Zoologische Staatssammlung München

Dirk Neumann, Sektion Ichthyologie / DNA-Labor

Münchhausenstr. 21

81247 München



Besuchen Sie unsere Sammlung:

https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=EDsY1V68uXff9kA8KEJteJfsBVa8fSDUogpS0C0J4Pc&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=CwMDaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=Zon_pRWu2LgaUZWryH53ux0AYw5xz6rO-vGC5OsQA_c&s=EsDqQFqkkW0MGjC44P59OoWs3ofclT-YiRGrCPksJsU&e=>



---------



Dirk Neumann



Tel: +49-89-8107-111

Fax: +49-89-8107-300

email: Dirk.Neumann(a)zsm.mwn.de



postal address:



Bavarian Natural History Collections

The Bavarian State Collection of Zoology

Dirk Neumann, Section Ichthyology / DNA-Lab

Muenchhausenstr. 21

81247 Munich (Germany)



Visit our section at:

https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=EDsY1V68uXff9kA8KEJteJfsBVa8fSDUogpS0C0J4Pc&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.zsm.mwn.de_ich_&d=CwMDaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=Zon_pRWu2LgaUZWryH53ux0AYw5xz6rO-vGC5OsQA_c&s=EsDqQFqkkW0MGjC44P59OoWs3ofclT-YiRGrCPksJsU&e=>






-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20161201/751dfc21/attachment.html