[Nhcoll-l] dna storage tubes for -80°C

Fri Dec 2 14:04:26 EST 2016

Dirk,

I vaguely recall a thread from several years ago about this very topic.  Someone had done some analysis and talked to the tube manufacturer and their conclusion was that the silicone gaskets did not seal in ethanol.  A search of the nh-coll archives might turn it up as I am clearly a bit vague on the details.  They did an assessment of a variety of tubes and recommended the Nalgene ones.  We’re using a slightly different variety than Andy https://urldefense.proofpoint.com/v2/url?u=https-3A__www.fishersci.com_shop_products_nalgene-2Dgeneral-2Dlong-2Dterm-2Dstorage-2Dcryogenic-2Dtubes_033377d&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=FuIWHY9LgLat3tvJWJJoITt0mNheD2FcX_K-z7UUpr0&s=-zq1envNU-Pmu6IQnvJpnzx-y3OpkUtkAqBoIgPxvzE&e= .  I’m not quite sure what the difference is but I know that what we’re using was the recommendation made at the time.

Best wishes- Jean

Jean L. Woods, Ph.D.                                                    Phone: 302-658-9111 x314
Curator of Birds                                                             Fax: 302-658-2610
Delaware Museum of Natural History                      jwoods at delmnh.org
P.O. Box 3937                                                                www.delmnh.org
(4840 Kennett Pike)
Wilmington, DE  19807

From: nhcoll-l-bounces at mailman.yale.edu [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Bentley, Andrew Charles
Sent: Thursday, December 01, 2016 2:22 PM
To: Dirk Neumann
Cc: nhcoll-l at mailman.yale.edu
Subject: Re: [Nhcoll-l] dna storage tubes for -80°C

Dirk

We have been using these for a number of years now - https://urldefense.proofpoint.com/v2/url?u=https-3A__www.fishersci.com_shop_products_nalgene-2Dgeneral-2Dlong-2Dterm-2Dstorage-2Dcryogenic-2Dtubes_033377w-3FsearchHijack-3Dtrue-26searchTerm-3D033377W-26searchType-3DRAPID&d=CwIGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=FuIWHY9LgLat3tvJWJJoITt0mNheD2FcX_K-z7UUpr0&s=3-iP6cWSSjTI7df3igNASU5IYKj0Px7cORISb7mofNo&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.fishersci.com_shop_products_nalgene-2Dgeneral-2Dlong-2Dterm-2Dstorage-2Dcryogenic-2Dtubes_033377w-3FsearchHijack-3Dtrue-26searchTerm-3D033377W-26searchType-3DRAPID&d=CwMGaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=VaZxKWWpo2bZ9QzRzukz9mEF2_Hnf4sT6yyuth1iy60&s=uQg2Y4uc188QnChKc072r4at7J4xXMUw5btRbaRin8s&e=>

We specifically selected ungasketed, externally threaded tubes even for -80 freezers which we were using at the time.  There were too many reports of gasket failure at low temps and externally threaded tubes, although taking up slightly more space in storage, negate any contamination issues that may occur by having an internally threaded closure potentially in contact with tissue or preservation fluid.

Hope that helps

Andy

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From: nhcoll-l-bounces at mailman.yale.edu<mailto:nhcoll-l-bounces at mailman.yale.edu> [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Dirk Neumann
Sent: Thursday, December 01, 2016 9:03 AM
To: nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>
Subject: [Nhcoll-l] dna storage tubes for -80°C

Hi all,

I would like to know if there are any specific experiences (good and bad) on DNA-storage tubes (normal freezing as we don't have liquid-N storage).

We are using Matrix racks (sepra seals) and I want to change to high quality skrew-lid tubes, but I am sceptical with the silicone gasket in combination with the internal thread of the tubes.

Thermo says that the tubes are tight sealing (from liquid-N to normal freezing and even room temperature), but if the gasket starts to crumble, then the tubes necks are open. Also, gaskets are made of silicone, which might cause issues with cold-flowing behaviour and life span of the material.

Curious to hear your thoughts, especially if you have experiences where things turned out to be not that good as originally promised by the manufacturer (also off-line, if you wouldn't like to post any failures to the broad public)

Thanks in advance for any helpful feedback

Dirk

--

Dirk Neumann

Tel: 089 / 8107-111

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email: Dirk.Neumann(a)zsm.mwn.de

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