[Nhcoll-l] dna storage tubes for -80°C

[Kevin Winker]

Hi Dirk et al. If you don't need to immerse in LN2, I recommend a cheaper
vial. We've been using these for almost 20 years with no complaints:
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.perf-2Dsci.com_d-5Fcryo_cryo.html&d=CwIFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=XzqgYbp54Oi5EuvAsh0DsGbNIOvJzTkWmPab1eT4F9s&s=uAsGP_q5AhmAxh46yiOtXx_9D81bv7g5rnkvRLUYhAU&e=  They work fine in -80 or
vapor-phase LN2.

When we do immerse in LN2 (e.g., in the field) we use more expensive vials
that can take that.

Best, K.

On Thu, Dec 1, 2016 at 6:03 AM, Dirk Neumann <dirk.neumann at zsm.mwn.de>
wrote:

> Hi all,
>
> I would like to know if there are any specific experiences (good *and*
> bad) on DNA-storage tubes (normal freezing as we don't have liquid-N
> storage).
>
> We are using Matrix racks (sepra seals) and I want to change to high
> quality skrew-lid tubes, but I am sceptical with the silicone gasket in
> combination with the internal thread of the tubes.
>
> Thermo says that the tubes are tight sealing (from liquid-N to normal
> freezing and even room temperature), but if the gasket starts to crumble,
> then the tubes necks are open. Also, gaskets are made of silicone, which
> might cause issues with cold-flowing behaviour and life span of the
> material.
>
> Curious to hear your thoughts, especially if you have experiences where
> things turned out to be not that good as originally promised by the
> manufacturer (also off-line, if you wouldn't like to post any failures to
> the broad public)
>
>
> Thanks in advance for any helpful feedback
>
> Dirk
>
> --
> Dirk Neumann
>
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> Fax: 089 / 8107-300
> email: Dirk.Neumann(a)zsm.mwn.de
>
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>
> ---------
>
> Dirk Neumann
>
> Tel: +49-89-8107-111 <+49%2089%208107111>
> Fax: +49-89-8107-300 <+49%2089%208107300>
> email: Dirk.Neumann(a)zsm.mwn.de
>
> postal address:
>
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> The Bavarian State Collection of Zoology
> Dirk Neumann, Section Ichthyology / DNA-Lab
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> 81247 Munich (Germany)
>
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-- 
Kevin Winker
University of Alaska Museum
907 Yukon Drive
Fairbanks, AK 99775
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