[Nhcoll-l] Rite-in-Rain Paper
Dirk Neumann
dirk.neumann at zsm.mwn.de
Wed Jan 4 16:39:25 EST 2017
Hi Alexandra,
I am not sure about the composition of RIR, but if uses formaldehyde
e.g. in the pulp to glue it together, the paper might be reactive if it
gets into contact with formalin, e.g. start to swell, get sticky / stick
together. I once tried a high quality archival paper and put a small
bundle of labels into formalin. As soon as they entered, they started to
swell and stuck firmly together; it was impossible to detach individual
labels from each other again.
But there are also other chemicals that could do harm (e.g. shifting the
pH). Normally, if a paper is some sort of water resistant, either the
surface and bottom and/or the pulp is hydrophobised. Normal paper
already uses up to 30 chemicals, waterproofed paper surely more. Even if
you use RIR only temporarily in the field until samples are returned,
you may already have a chemical cocktail inside because 96% EthOH is a
fairly good organic solvent, too.
For me, externally pre-numbered tubes seem the safer option; or tubes
that can be marked externally with pencils. If those internally RIR
tagged samples fail during extraction or give bad sequencing results,
you add just one more unknown to the black box of weird lab mysteries
making samples fail.
I guess the only other option would be to specifically test for effects
of internal RIR tags by subdividing a tissue to two tubes, one
externally labelled, the other with internal RIR, returned under
identical conditions to the lab, and compare e.g. DNA yields after
extraction, PCR results, etc. if you want to be sure. For sure still not
the answer you are looking for, but hope it offers some sort of help.
All the best
Dirk
Am 04.01.2017 um 21:20 schrieb Alexandra Snyder:
> Andy, Absolutely. No disagreement there, i.e., external labels.
> Thanks for reminding the list.
>
> I would still like to document the condition of tissue accessions
> rec'd with RIR labels and the USFWS hatchery folks are curious re: the
> use of Rite in Rain for temporary labels.
>
> If there is a way to get an idea or if anyone has knowledge of RIR
> properties, I would like to hear about it. I recognize that
> manufacturers do not share recipes, but one never knows.
>
> Lex
>
> On Wed, Jan 4, 2017 at 11:41 AM, Bentley, Andrew Charles
> <abentley at ku.edu <mailto:abentley at ku.edu>> wrote:
>
> Lex
>
> We have a policy here at KU that nothing gets put inside the tube
> except the tissue and preservative (if necessary). There are just
> too many possible avenues of contamination from the “paper” to the
> “ink” to the residue left on the paper by human contact. All
> labels are external and usually adhered before going in to the
> field using a Brady labeling system and cryo safe labeling material.
>
> Andy
>
> A : A : A :
>
> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>
> V V V
>
> Andy Bentley
>
> Ichthyology Collection Manager
>
> University of Kansas
>
> Biodiversity Institute
>
> Dyche Hall
>
> 1345 Jayhawk Boulevard
>
> Lawrence, KS, 66045-7561
>
> USA
>
> Tel: (785) 864-3863 <tel:%28785%29%20864-3863>
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>
> Email: abentley at ku.edu <mailto:abentley at ku.edu>
>
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> : :
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> A : A : A :
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> }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
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> V V V
>
> *From: *<nhcoll-l-bounces at mailman.yale.edu
> <mailto:nhcoll-l-bounces at mailman.yale.edu>> on behalf of Alexandra
> Snyder <amsnyder at unm.edu <mailto:amsnyder at unm.edu>>
> *Date: *Wednesday, January 4, 2017 at 12:10 PM
> *To: *"nhcoll-l at mailman.yale.edu
> <mailto:nhcoll-l at mailman.yale.edu>" <nhcoll-l at mailman.yale.edu
> <mailto:nhcoll-l at mailman.yale.edu>>
> *Subject: *[Nhcoll-l] Rite-in-Rain Paper
>
> Rite-in-Rain or "all weather" field paper is used by the many
> agency biologists depositing specimens and genetic samples with
> the MSB Division of Fishes and other repositories. Recently, the
> USFWS Southwestern Native Aquatic Resources and Recovery Center
> has requested that I find out if there are any issues with using
> this paper for genetic labels in the field or otherwise. They
> tried to get information from the Rite-in-Rain company re: what
> was in the paper and coating (formalin?) to determine if the paper
> would pose a problem for genetic samples. The company was not
> forthcoming re: Rite-in-Rain product specifics.
>
> We have had many genetic samples received at both the Center and
> the MSB with these paper tags made by tearing pieces of paper out
> of the RIR field books, writing the sample number on RIR tag with
> pencil, and stuffing the tag into a cryotube. Not optimal in
> terms of contamination issues, etc. but getting word out to field
> crews, etc. can be difficult. For many projects, I preprint
> labels on polyspun paper (DataMax) for researchers, but there are
> many opportunistic collections that receive these temporary labels.
>
> Any thoughts or insights? Does anyone have an idea of how this
> paper is manufactured/treated?
>
> Thank you.
>
> *************************************
>
> Alexandra M Snyder, Collections Manager-Fishes
>
> Museum of Southwestern Biology MSC03-2020
>
> University of New Mexico Albuquerque NM 87131
>
> PH./FAX 505.277.6005 <tel:%28505%29%20277-6005> amsnyder at unm.edu
> <mailto:amsnyder at unm.edu>
>
> PHYSICAL ADDRESS FOR FEDEX/UPS
>
> Bldg.83 Room 204
>
> 302 Yale Blvd NE
>
>
>
>
> --
> *****************************************************
> Alexandra M Snyder
> Collections Manager-Fishes
> Museum of Southwestern Biology MSC01-2020
> University of New Mexico
> Albuquerque NM 87131 USA
>
> PH.505.277.6005 OFFICE
>
> _Physical address for FedEx and UPS_
> Bldg.83 Room 204, 302 Yale Blvd NE
>
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--
Dirk Neumann
Tel: 089 / 8107-111
Fax: 089 / 8107-300
email: Dirk.Neumann(a)zsm.mwn.de
Postanschrift:
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