[Nhcoll-l] eDNA voucher question

[Bentley, Andrew Charles]

Tom

We have used vials within jars to keep tissue vouchers separate and prevent cross-contamination.  You can vary the vials size based on the size of the fish and as such it shouldn't take up too much more space - even though it will take up a lot more of your time :).  Having said that I wonder how much cross-contamination you would get after the fish have been preserved?  Not sure that there would be much floating around in the liquid after preservation that wouldn't be drowned out by sending a tissue sample from the original fish for analysis.

My two cents...

Andy
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Andy Bentley
Ichthyology Collection Manager
University of Kansas
Biodiversity Institute
Dyche Hall
1345 Jayhawk Boulevard<x-apple-data-detectors://9>
Lawrence, KS, 66045-7561<x-apple-data-detectors://9>
USA<x-apple-data-detectors://9>

Tel: (785) 864-3863<tel:%28785%29%20864-3863>
Fax: (785) 864-5335<tel:%28785%29%20864-5335>
Email: abentley at ku.edu<mailto:abentley at ku.edu>
http://ichthyology.biodiversity.ku.edu<http://ichthyology.biodiversity.ku.edu/>
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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> On Behalf Of Thomas Labedz
Sent: Thursday, February 21, 2019 3:42 PM
To: NHCOLL-L at mailman.yale.edu
Subject: [Nhcoll-l] eDNA voucher question

Greetings from snowy central North America!
Has anyone developed policy or protocol for the receipt, treatment, and maintenance of eDNA fish voucher specimens? Would you share? The individual fish are small and the researcher recommends keeping each individual in a separate container (how they were delivered) to prohibit cross contamination of DNA via the ethanol solution. That is understandable but will utilize lots of space and containers.
Thomas
Thomas E. Labedz, Collections Manager
Division of Zoology and Division of Botany
University of Nebraska State Museum
Lincoln, Nebraska, U.S.A.
tlabedz1 at unl.edu<mailto:tlabedz1 at unl.edu>

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