[Nhcoll-l] eDNA voucher question

[Callomon,Paul]

Hi Andy,

What kind of stopper do you use in vials that are submerged in fluid? Presumably they must be impermeable, to prevent cross-contamination, but does e. g. polypropylene last well under full immersion? I know that phenolic does not - it will leach into alcohol after only a few years.

PC

Paul Callomon
Collection Manager, Malacology and General Invertebrates
________________________________
Academy of Natural Sciences of Drexel University, Philadelphia
1900 Benjamin Franklin Parkway, Philadelphia PA 19103-1195, USA
callomon at ansp.org<mailto:callomon at ansp.org> Tel 215-405-5096 - Fax 215-299-1170



From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> On Behalf Of Bentley, Andrew Charles
Sent: Friday, February 22, 2019 10:01 AM
To: Thomas Labedz <tlabedz1 at unl.edu>; NHCOLL-L at mailman.yale.edu
Subject: Re: [Nhcoll-l] eDNA voucher question

Tom

We have used vials within jars to keep tissue vouchers separate and prevent cross-contamination.  You can vary the vials size based on the size of the fish and as such it shouldn't take up too much more space - even though it will take up a lot more of your time :).  Having said that I wonder how much cross-contamination you would get after the fish have been preserved?  Not sure that there would be much floating around in the liquid after preservation that wouldn't be drowned out by sending a tissue sample from the original fish for analysis.

My two cents...

Andy
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Andy Bentley
Ichthyology Collection Manager
University of Kansas
Biodiversity Institute
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Email: abentley at ku.edu<mailto:abentley at ku.edu>
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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu<mailto:nhcoll-l-bounces at mailman.yale.edu>> On Behalf Of Thomas Labedz
Sent: Thursday, February 21, 2019 3:42 PM
To: NHCOLL-L at mailman.yale.edu<mailto:NHCOLL-L at mailman.yale.edu>
Subject: [Nhcoll-l] eDNA voucher question

Greetings from snowy central North America!
Has anyone developed policy or protocol for the receipt, treatment, and maintenance of eDNA fish voucher specimens? Would you share? The individual fish are small and the researcher recommends keeping each individual in a separate container (how they were delivered) to prohibit cross contamination of DNA via the ethanol solution. That is understandable but will utilize lots of space and containers.
Thomas
Thomas E. Labedz, Collections Manager
Division of Zoology and Division of Botany
University of Nebraska State Museum
Lincoln, Nebraska, U.S.A.
tlabedz1 at unl.edu<mailto:tlabedz1 at unl.edu>

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