[Nhcoll-l] Techniques for taking toe pad samples?

[Pepijn Kamminga]

Dear dr. Cassidy,

come to think of it, I have a tiny scissors in my toolkit, which has a very
small blade (max 1 cm) and is in an angle relative to the handle, that
gives you also more freedom of movement and has worked for me a few times
with small toepads. It look something like this:
[image: image.png]

Met vriendelijke groet /  Kind regards,

Pepijn Kamminga
Senior Collection Manager Birds & Mammals






+31717519338 - -
pepijn.kamminga at naturalis.nl - www.naturalis.nl
Darwinweg 2, 2333 CR Leiden
Postbus 9517, 2300 RA Leiden

<https://www.naturalis.nl/over-ons> <https://www.naturalis.nl/lang-leve>











Op wo 20 jan. 2021 om 16:55 schreef Cassidy, Kelly Michela <cassidyk at wsu.edu
>:

> Thank you. I generally don’t have issues taking skin clips. It’s the toe
> pads that are a challenge to sample without putting stress on the study
> skin. I received another request for toe pads (on kingfishers) this
> morning. Maybe some sort of tiny nippers would work better than a scalpel?
>
>
>
> Dr. Kelly M. Cassidy, Curator, Conner Museum
>
> School of Biological Sciences
>
> Box 644236
>
> Washington State University
>
> Pullman, WA 99164-4236
>
> 509-335-3515
>
>
>
> *From:* Pepijn Kamminga <pepijn.kamminga at naturalis.nl>
> *Sent:* Wednesday, January 20, 2021 6:35 AM
> *To:* Cassidy, Kelly Michela <cassidyk at wsu.edu>
> *Cc:* nhcoll-l at mailman.yale.edu
> *Subject:* Re: [Nhcoll-l] Techniques for taking toe pad samples?
>
>
>
> Dear dr. Cassidy,
>
>
>
> Similar requests are made for the bird and mammal collection of Naturalis
> Biodiversity Center, the Netherlands. Bird skins often have small flaps of
> skin that can be cut with fresh scalpel blades. For me it helps to apply
> some counterpressure, but the blade should do the cutting, so I don't apply
> too much force. As for mammals I prefer to use specimens stored on alcohol,
> I make a small incision in the ventral side of the skin and take a sample
> of the breast muscle. Mammalian toepads I find difficult in general. If the
> requested specimen is a skin, cutting a piece of tissue along the seam can
> also do the trick. I hope this is of any help to you.
>
>
>
> Met vriendelijke groet /  Kind regards,
>
>
>
> Pepijn Kamminga
>
> Senior Collection Manager Birds & Mammals
>
>
>
>
>
>
>
> +31717519338 - -
>
> pepijn.kamminga at naturalis.nl - www.naturalis.nl
> <https://urldefense.com/v3/__https:/www.naturalis.nl__;!!JmPEgBY0HMszNaDT!-MEoFpA5aTMtbFqkZxBhK2QCzCz_yq7CPSVoQwY7JXE1dZe0iCzcOb7empd--KQ$>
>
> Darwinweg 2, 2333 CR Leiden
>
> Postbus 9517, 2300 RA Leiden
>
>
>
>
> <https://urldefense.com/v3/__https:/www.naturalis.nl/lang-leve__;!!JmPEgBY0HMszNaDT!-MEoFpA5aTMtbFqkZxBhK2QCzCz_yq7CPSVoQwY7JXE1dZe0iCzcOb7ePk4zTL8$>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> Op di 19 jan. 2021 om 19:27 schreef Cassidy, Kelly Michela <
> cassidyk at wsu.edu>:
>
> I am getting more frequent requests for toe pad samples for DNA
> sequencing, instead of skin clips.
>
>
>
> I find it difficult to take these samples without putting stress on the
> prepared skin. The toe pads are tough and leathery. Scalpels don’t cut into
> them very easily, if at all. The most recent request is for a sample from a
> vole, which has small feet.
>
>
>
> Are there any techniques or recommended tools to help minimize stress and
> possible damage to skins when taking toe pad samples?
>
>
>
> Dr. Kelly M. Cassidy, Curator, Conner Museum
>
> School of Biological Sciences
>
> Box 644236
>
> Washington State University
>
> Pullman, WA 99164-4236
>
> 509-335-3515
>
>
>
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