[Nhcoll-l] Fluid preservation correction

Thu May 20 14:52:07 EDT 2021

Julian

That is presuming that the specimens were not first fixed in formalin before being placed in 95% ethanol.

Andy

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Andy Bentley
Ichthyology Collection Manager
University of Kansas
Biodiversity Institute
Dyche Hall
1345 Jayhawk Boulevard
Lawrence, KS, 66045-7561
USA

Tel: (785) 864-3863
Fax: (785) 864-5335
Email: abentley at ku.edu<mailto:abentley at ku.edu>
http://ichthyology.biodiversity.ku.edu<http://ichthyology.biodiversity.ku.edu/>

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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> on behalf of "Julian uk>" <Julian.Carter at museumwales.ac.uk>
Date: Thursday, May 20, 2021 at 1:51 PM
To: Joosep Sarapuu <Joosep.Sarapuu at loodusmuuseum.ee>, "nhcoll-l at mailman.yale.edu" <nhcoll-l at mailman.yale.edu>
Subject: Re: [Nhcoll-l] Fluid preservation correction

Hi Joosep,

96% Ethanol (with no additives) will actually be better for DNA preservation as it effectively dehydrates the specimen and reduces hydrolytic effects. So I’d keep them in the 96% EtOH. ‘Cold and dry’ tends to be best for biomolecule preservation.

As to the denatured EtOH – then probably best to transfer into equivalent absolute ethanol. The ‘denaturant’ used can be very variable though usually something like MEK or Methanol, both of which are better avoided with DNA samples.

Hope that helps.

All the best,

Jules

Julian Carter (he/him)
Prif Gadwraethydd, Gwyddorau Naturiol / Principal Conservator Natural Sciences
julian.carter at amgueddfacymru.ac.uk<mailto:julian.carter at amgueddfacymru.ac.uk>
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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> On Behalf Of Joosep Sarapuu
Sent: 20 May 2021 14:12
To: nhcoll-l at mailman.yale.edu
Subject: [Nhcoll-l] Fluid preservation correction

Dear all,

I have a question about fluid preservation correction.

  1.  I have three samples of animals in 96% EtOH right now, but i know that the best for DNA would be 75% EtOH. Can i just dilute this to the right amount or i have to wash it in destilled water and then step by step to 75% EtOH?
  2.  I have one animal in denaturated EtOH. This one i have to wash and then also step by step to 75% normal EtOH?

Sincerely,
Joosep Sarapuu

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