[Nhcoll-l] Advice on removing specimens from glass tubes

Fabian Neisskenwirth info at naturhistorische-konservierung.de
Tue Mar 1 04:22:54 EST 2022


Hey Luisa,


I agree with Andrew's suggestion of placing them on ethafoam. I attached 
a picture of some similar specimens I rehoused for storage in a school 
collection in Wuppertal.

As long as the tubes show no leakage and the specimens are stable, I see 
no reason of rehousing them. They look very well preserved to me. Of 
course you should keep an eye on the cork/rubber stopper.


... and to James question,

the method that John points out works very well. I did it using a 
portable Raman-Spectrometer provided by Ocean Insight 
(https://www.oceaninsight.com/products/measurement--technique-bundles/raman-bundle-785/?qty=1) 
during my master thesis on the conservation of fluid preserved 
specimens. I encourage everyone who could afford such analysis to try it 
out, we need a big spectral library of references so its easier to 
identify possible preservation fluids ;>)

For more info get in contact with Sophie.


All best,

Am 28.02.22 um 23:06 schrieb John E Simmons:
> A recent paper has reported on the use of Raman spectrometry for this 
> purpose, but you need the right lab equipment to do use it. Sophie 
> Cersoy demonstrated the technique for us in Paris during the 2018 
> fluid collection conference, and her paper is now available:
>
> S. Cersoy, V. Rouchon, O. Belhadj, J. Cuisin, and M. Herbin. 2020. 
> Noninvasive fluid identification: potential of micro-Raman 
> spectroscopy. /Collection Forum/ 34(1):53-72
>
> --John
>
> John E. Simmons
> Writer and Museum Consultant
> Museologica
> /and/
> Associate Curator of Collections
> Earth and Mineral Science Museum & Art Gallery
> Penn State University
> /and/
> Investigador Asociado, Departamento de Ornitologia
> Museo de Historia Natural, Universidad Nacional Mayor de San Marcos, Lima
>
>
> On Mon, Feb 28, 2022 at 5:00 PM James Bryant <jbandjb at live.com> wrote:
>
>     Turtox! Fascinating, John. I agree that it would be useful to know
>     how old these preparations might be. If they’ve remained stable
>     this long, I can’t imagine there are many other reasons to disturb
>     them.
>
>     Perhaps I’ve just not recalling things, but are there any
>     instrumental methods to analyze the content of solutions used in
>     fluid collections without disturbing the containers?
>
>     James Bryant
>     SOJOURN Science - Nature - Education
>     Santa Fe, NM
>     https://www.linkedin.com/in/james-bryant-0598a940/
>
>
>>     On Feb 28, 2022, at 1:00 PM, John E Simmons
>>     <simmons.johne at gmail.com> wrote:
>>
>>     This technique was published in Turtox News 15(10:129 in October
>>     of 1937 in an anonymous short article with the title "A method of
>>     displaying snakes." The article includes a photograph of a rack
>>     holding a number of long tubes with preserved snakes stretched
>>     out in them, sent in by "Professor John M. Frazier of the State
>>     Teachers College, Hattiesburg Mississippi." Prof. Frazier
>>     reported that "The snakes are injected with formalin-alcohol
>>     preservative and are hardened instraight and extended position.
>>     They are then inserted in the glass tubes, the ends of which are
>>     sealed with cork or rubber stoppers and coated with paraffin
>>     after the tubes have been completely filled with the preserving
>>     solution."
>>
>>     There were several "formalin-alcohol preservative" mixtures that
>>     were popular at the time, the idea being that you could reduce
>>     the two-steps of fixation and preservation into one. These
>>     mixtures were not successful because the chemical actions of the
>>     formaldehyde and alcohol interfered with each other, resulting in
>>     uneven preservation as tissues were dehydrated. For example, one
>>     mixture called for 95ml of 70% ETOH and 5 ml of formaldehyde;
>>     another for 50 parts alcohol, 5 parts formaldehyde, and 45 parts
>>     water. It may also refer to what was more commonly called FAA,
>>     which was formaldehyde, alcohol, and acetic acid.
>>
>>     You cannot tell just by looking what solution the specimens are
>>     in, but I expect it is alcohol due to the discoloration
>>     (formaldehyde does not extract lipids as readily as alcohol).
>>     However, I would handle these as if they did contain formaldehyde
>>     and take appropriate precautions until you are sure. The problem
>>     with re-housing the specimens will be that they are going to be
>>     very stiff and it will be difficult to coil them up without
>>     damaging them. If they are not leaking, and you do not need to
>>     remove the specimens for examination, I would leave them as they
>>     are but house the tubes in a way that will reduce the chance of
>>     breakage, such as in a box or tray with half-rounds of cardboard
>>     to keep them from rolling or touching each other. They are an
>>     excellent example of an old technique that was rather quaint.
>>
>>     Any idea when the specimens were preserved?
>>
>>     --John
>>
>>     John E. Simmons
>>     Writer and Museum Consultant
>>     Museologica
>>     /and/
>>     Associate Curator of Collections
>>     Earth and Mineral Science Museum & Art Gallery
>>     Penn State University
>>     /and/
>>     Investigador Asociado, Departamento de Ornitologia
>>     Museo de Historia Natural, Universidad Nacional Mayor de San
>>     Marcos, Lima
>>
>>
>>     On Mon, Feb 28, 2022 at 2:02 PM Luisa Zamora Chavez
>>     <lzamorac at asu.edu> wrote:
>>
>>         Hello all,
>>
>>         I have a few liquid-preserved snakes in glass tubes that were
>>         donated to our collections sometime ago. The tubes are sealed
>>         shut using what appears to be plastic corks, tape, and
>>         sealant. We're not sure if the liquid they're in is formalin
>>         or something other than ethanol.
>>
>>         I am wondering if anyone has had any experience with this
>>         sort of preservation and any advice on how to transfer the
>>         specimens to a more stable mode? We'd like to keep some of
>>         them but fear the tubes might break. We are unsure of what
>>         liquid is typically used for this type of preservation and
>>         would like to be as prepared as possible so we can
>>         safely remove them from the tubes (if that is at all
>>         possible). I have included some pictures of the specimens for
>>         reference. Any help is greatly appreciated!
>>
>>         Kind regards,
>>         Luisa
>>
>>
>>         -- 
>>         *Luisa Zamora Chavez *
>>         Pronouns: she/they
>>         Research Technician
>>         Arizona State University Biocollections
>>         Lzamorac at asu.edu
>>         602-737-8357
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>>     _______________________________________________
>>     NHCOLL-L is brought to you by the Society for the Preservation of
>>     Natural History Collections (SPNHC), an international society whose
>>     mission is to improve the preservation, conservation and
>>     management of
>>     natural history collections to ensure their continuing value to
>>     society. See http://www.spnhc.org for membership information.
>>     Advertising on NH-COLL-L is inappropriate.
>
>
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> _______________________________________________
> NHCOLL-L is brought to you by the Society for the Preservation of
> Natural History Collections (SPNHC), an international society whose
> mission is to improve the preservation, conservation and management of
> natural history collections to ensure their continuing value to
> society. Seehttp://www.spnhc.org  for membership information.
> Advertising on NH-COLL-L is inappropriate.
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