[Nhcoll-l] DICE-CT on NHspecimens

Thu Mar 24 15:14:05 EDT 2022

In case it's helpful, here are a few papers with different concentrations
for different specimens, and one that used diceCT and then did histology.
It's destructive in the sense that, regardless of "de-staining" with sodium
thiosulfate, you'll still end up with iodine remaining in the specimen (but
it won't be red anymore!) You can at least remove the red color, and
specimens are still amenable to later histology, etc. The shrinkage can be
mitigated well with Sorensen's buffer, and specimens are then stable in
ethanol. (I mean, it's a new-ish protocol, so who knows what they'll look
like in 100 years, but I don't know why it'd be much different from any
other specimen in alcohol!)
In addition to talking with Jaimi Gray, I also recommend talking to
Catherine Early, Paul Gignac, and Aki Watanabe - all super knowledgeable
about diceCT techniques for vertebrate specimens.

Allison

On Wed, Mar 23, 2022 at 1:28 PM Randall, Zachary <zrandall at flmnh.ufl.edu>
wrote:

> The biggest issue with diceCT has been soft-tissue shrinkage, but this has
> been remedied by using Sorensen’s buffer as published by Dawood et al.
> 2021. This is the buffer used in the Gray protocol shared by Andy. Links to
> the Dawood paper as well as others of potential interest are below.
>
>
>
> Dawood et al. 2021: https://www.nature.com/articles/s41598-021-99202-2
>
> Early et al. 2020:
> https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238783
>
> Hedrick et al. 2018:
> https://www.researchgate.net/publication/325862458_Assessing_Soft-Tissue_Shrinkage_Estimates_in_Museum_Specimens_Imaged_With_Diffusible_Iodine-Based_Contrast-Enhanced_Computed_Tomography_diceCT
>
>
>
> Best,
>
> Zach
>
> --
>
> *Zachary S. Randall*
>
> Digital Imaging Manager
>
> Florida Museum of Natural History
>
> 1659 Museum Road, Gainesville, FL 32611-7800
>
> (352) 273-1958 | zrandall at flmnh.ufl.edu
>
>
>
> https://zachrandall.weebly.com/
>
> www.floridamuseum.ufl.edu/digital-lab/
>
> https://sketchfab.com/FloridaMuseum
>
>
>
>
>
> *From:* Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> * On Behalf Of *Bentley,
> Andrew Charles
> *Sent:* Wednesday, March 23, 2022 10:01 AM
> *To:* Esther Dondorp <esther.dondorp at naturalis.nl>;
> nhcoll-l at mailman.yale.edu
> *Subject:* Re: [Nhcoll-l] DICE-CT on NHspecimens
>
>
>
> *[External Email]*
>
> Esther
>
>
>
> The oVert project here in the US has had extensive experience with diceCT
> and have developed the attached protocol.  Jaimi Gray would be the person
> to contact in this regard - jaimigray at floridamuseum.ufl.edu.  She also
> co-authored the following publication -
> https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__onlinelibrary.wiley.com_doi_full_10.1002_ece3.7467&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=PCoqPP0RplincQmDRfDfKbAf08gmtmu0zMnevmF7kYE&e=>
>
>
>
> Hope that helps
>
>
>
> Andy
>
>     A  :             A  :             A  :
>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>     V                V                V
> Andy Bentley
> Ichthyology Collection Manager
> University of Kansas
> Biodiversity Institute
>
> Dyche Hall
> 1345 Jayhawk Boulevard
> Lawrence, KS, 66045-7561
> USA
>
> Tel: (785) 864-3863
> Fax: (785) 864-5335
> Email: abentley at ku.edu
>
> ORCID: https://orcid.org/0000-0002-3093-1258
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__orcid.org_0000-2D0002-2D3093-2D1258&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=W6K85Ze9PmgioL9GHoEqKgA6yf-ISXmJ6QyjpUy0Uyw&e=>
>
> http://ichthyology.biodiversity.ku.edu
> <https://urldefense.proofpoint.com/v2/url?u=http-3A__ichthyology.biodiversity.ku.edu_&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=ANS6f0lN9_8S28FjQKr0jwy3geQCl1FB4n7T1ALKhwg&e=>
>
>     A  :             A  :             A  :
>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>     V                V                V
>
>
>
> *From:* Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> * On Behalf Of *Esther
> Dondorp
> *Sent:* Wednesday, March 23, 2022 4:24 AM
> *To:* nhcoll-l at mailman.yale.edu
> *Subject:* [Nhcoll-l] DICE-CT on NHspecimens
>
>
>
> Dear colleagues,
>
>
>
> Does anyone have experience with Diffusible Iodine-based
> Contrast-enhanced Computed Tomography (DICE-CT) performed on fluid
> preserved natural history specimens? I want to know if it is really non
> destructive, if the specimens do stay stable for the next many years or
> more after this treatment?
>
>
>
> Many thanks!
>
>
>
> Esther Dondorp
>
> Senior Collectiebeheerder
>
> [image: Image removed by sender.]
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__nam10.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Fnaturalis-2Dpublic-2Dmedia-2Dassets.s3.eu-2Dcentral-2D1.amazonaws.com-252FNaturalis-2Dmail-2Dsignature-252Flogo.png-26data-3D04-257C01-257Cabentley-2540ku.edu-257C045b40042878448dbb0208da0caef50d-257C3c176536afe643f5b96636feabbe3c1a-257C0-257C0-257C637836242826560921-257CUnknown-257CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0-253D-257C3000-26sdata-3D9F3QxE2YraYusdBUWfenHfsfCdHXPhXAQ4PcmIBbZkk-253D-26reserved-3D0&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=RYSSbVNkyqIqkc1C-VlPbEfSBXyqzx7etNMkIzMCpGk&e=>
>
>
>
>
>
>
> - -
> esther.dondorp at naturalis.nl - www.naturalis.nl
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__nam10.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Fwww.naturalis.nl-252F-26data-3D04-257C01-257Cabentley-2540ku.edu-257C045b40042878448dbb0208da0caef50d-257C3c176536afe643f5b96636feabbe3c1a-257C0-257C0-257C637836242826560921-257CUnknown-257CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0-253D-257C3000-26sdata-3Dy-252B43gsCV3bwJk4g4Wdm06Z8fbmHMM1HfR20aqEGlWBQ-253D-26reserved-3D0&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=adbMMe-pIUO8NhDu0IUIdOuKJcJMbH9yn8KRx5h8nVY&e=>
> Darwinweg 2, 2333 CR Leiden
> Postbus 9517, 2300 RA Leiden
>
> [image: Image removed by sender.]
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__nam10.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Fwww.naturalis.nl-252F-26data-3D04-257C01-257Cabentley-2540ku.edu-257C045b40042878448dbb0208da0caef50d-257C3c176536afe643f5b96636feabbe3c1a-257C0-257C0-257C637836242826560921-257CUnknown-257CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0-253D-257C3000-26sdata-3Dy-252B43gsCV3bwJk4g4Wdm06Z8fbmHMM1HfR20aqEGlWBQ-253D-26reserved-3D0&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=adbMMe-pIUO8NhDu0IUIdOuKJcJMbH9yn8KRx5h8nVY&e=>
>
>
> _______________________________________________
> Nhcoll-l mailing list
> Nhcoll-l at mailman.yale.edu
> https://mailman.yale.edu/mailman/listinfo/nhcoll-l
>
> _______________________________________________
> NHCOLL-L is brought to you by the Society for the Preservation of
> Natural History Collections (SPNHC), an international society whose
> mission is to improve the preservation, conservation and management of
> natural history collections to ensure their continuing value to
> society. See http://www.spnhc.org for membership information.
> Advertising on NH-COLL-L is inappropriate.
>

-- 
Dr. Allison W. Bronson
Adjunct Faculty and Research Associate
Department of Biological Sciences
California State Polytechnic University, Humboldt
https://sites.google.com/humboldt.edu/fish-anatomy
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20220324/5f5bc5df/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: ~WRD0005.jpg
Type: image/jpeg
Size: 823 bytes
Desc: not available
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20220324/5f5bc5df/attachment-0001.jpg>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: Gignac--(cover) Multiscale imaging of the rat brain using an integrated diceCT and histology workflow.pdf
Type: application/pdf
Size: 7058193 bytes
Desc: not available
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20220324/5f5bc5df/attachment-0003.pdf>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: Metscher2009_StainingMicroCT.pdf
Type: application/pdf
Size: 1607700 bytes
Desc: not available
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20220324/5f5bc5df/attachment-0004.pdf>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: GignacKley2014.pdf
Type: application/pdf
Size: 8952744 bytes
Desc: not available
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20220324/5f5bc5df/attachment-0005.pdf>