[Nhcoll-l] cleared and stained amphibians

[Fabian Neisskenwirth]

Hey Tonya,

I would recommend to measure the density of the solution before assuming 
anything. With this you can calculate what percentage of strength you 
can expect. Usually this can vary very much from literature, to 
literature. And i really have never heard the use of ethanol in 
clearings. The ones I have made are all in plain glycerol and water and 
are preserved very well.

And I would not add any thymol, since if the solution is above 65% mold 
growth is inhibited. Why use poison if not needed...


All the best,


Am 06.10.22 um 04:32 schrieb Bentley, Andrew Charles:
>
> Tonya
>
> We have sometimes mixed different proportions of glycerin and 70% 
> ethanol to make the liquid less viscous and thus reduce bubble 
> formation in cavities of C&S specimens but otherwise use full strength 
> glycerin for these specimens with a couple of crystals of thymol added 
> to prevent mold growth.  The addition of 70% ethanol, as long as not 
> too much, does not seem to affect these specimens in any adverse way – 
> that we can tell.
>
> Andy
>
>   A  :             A  :             A  :
>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>     V                V                V
> Andy Bentley
> Ichthyology Collection Manager
> University of Kansas
> Biodiversity Institute
>
> Dyche Hall
> 1345 Jayhawk Boulevard <x-apple-data-detectors://9/>
> Lawrence, KS, 66045-7561 <x-apple-data-detectors://9/>
> USA <x-apple-data-detectors://9/>
>
> Tel: (785) 864-3863 <tel:%28785%29%20864-3863>
> Fax: (785) 864-5335 <tel:%28785%29%20864-5335>
> Email: abentley at ku.edu <mailto:abentley at ku.edu>
>
> ORCID: https://orcid.org/0000-0002-3093-1258 
> <https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Forcid.org%2F0000-0002-3093-1258&data=04%7C01%7Cabentley%40ku.edu%7C5720c0e4d0f94b9e0df008da0127752c%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637823566221065460%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=LFnPbiFA9HMZ8k85%2F0%2FOx5vJxTNhZTjAo7S%2BqrDAh6c%3D&reserved=0>
>
> http://ichthyology.biodiversity.ku.edu 
> <http://ichthyology.biodiversity.ku.edu/>
>
>     A :             A  :             A  :
>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>     V                V                V
>
> *From: *Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> on behalf of 
> "Haff, Tonya (NCMI, Crace)" <Tonya.Haff at csiro.au>
> *Date: *Wednesday, October 5, 2022 at 6:13 PM
> *To: *"nhcoll-l at mailman.yale.edu" <nhcoll-l at mailman.yale.edu>
> *Subject: *[Nhcoll-l] cleared and stained amphibians
>
> Hello all,
>
> We have some cleared and stained small frogs that are, I suspect, 
> stored in a mixture of glycerine and ethanol. I have read in John’s 
> Fluid Preservation book that some people use a mixture the two at a 
> 1:1 ratio (but at what ETOH concentration I am not sure), but I wonder 
> if any of you would share how you store similar specimens? Some of the 
> jars need to be topped up, so I also wonder if it’s best to top up 
> using a 1:1 ratio of glycerine to ethanol (given that’s what it 
> **looks** like, ha ha), or remove all fluid and replace, or…? 
> Thoughts, as usual, appreciated!
>
> Cheers,
>
> Tonya
>
> -------------------------------------------------
>
> Dr. Tonya M. Haff
>
> Collection Manager
>
> Australian National Wildlife Collection
>
> CSIRO
>
> +61(0)419569109
>
> https://www.csiro.au/en/about/facilities-collections/collections/anwc 
> <https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.csiro.au%2Fen%2Fabout%2Ffacilities-collections%2Fcollections%2Fanwc&data=05%7C01%7Cabentley%40ku.edu%7C0ef503da722a44a5569c08daa727284c%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C638006083885233690%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=xo7oQ1sFwdNOMO0Cpp68SkGfv2l0GX0YAd06SULP9mc%3D&reserved=0>
>
>
> _______________________________________________
> Nhcoll-l mailing list
> Nhcoll-l at mailman.yale.edu
> https://mailman.yale.edu/mailman/listinfo/nhcoll-l
>
> _______________________________________________
> NHCOLL-L is brought to you by the Society for the Preservation of
> Natural History Collections (SPNHC), an international society whose
> mission is to improve the preservation, conservation and management of
> natural history collections to ensure their continuing value to
> society. Seehttp://www.spnhc.org  for membership information.
> Advertising on NH-COLL-L is inappropriate.
-- 

Tel: +49 (0) 1573 2778729

Web: www.naturhistorische-konservierung.de
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20221006/1ab4f4cf/attachment.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: Logo.jpg
Type: image/jpeg
Size: 47337 bytes
Desc: not available
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20221006/1ab4f4cf/attachment.jpg>