[Nhcoll-l] [MOGELIJK SPAM ! ********] 95% vs. ~70% EtOH?

A.J.van_Dam at lumc.nl A.J.van_Dam at lumc.nl
Tue Jul 29 04:56:40 EDT 2014


Dear Sonia,

In general one could say:

95% is better for DNA
70% is better for morphology
Ethanol is better than IMS

Regards,

Dries
Andries J. van Dam, conservator

Museum of Anatomy
Leiden University Medical Center
Building 3 (V3-32)
P.O.Box 9600
2300 RC Leiden
The Netherlands
tel: +31 (0)71 52 68356
E-mail: A.J.van_Dam at lumc.nl<mailto:A.J.van_Dam at lumc.nl>
Visiting address: Hippocratespad 21
Scientific associate Natural History Museum London
http://www.nhm.ac.uk<http://www.nhm.ac.uk/>
Directory Board member ICOM-CC
http://www.icom-cc.org<http://www.icom-cc.org/>

Director Alcomon Company
http://www.alcomon.com<http://www.alcomon.com/>



From: nhcoll-l-bounces at mailman.yale.edu [mailto:nhcoll-l-bounces at mailman.yale.edu] On Behalf Of Sonia Rowley
Sent: dinsdag 29 juli 2014 10:32
To: nhcoll-l at mailman.yale.edu
Subject: [MOGELIJK SPAM ! ********] [Nhcoll-l] 95% vs. ~70% EtOH?

Dear List

I am writing with regards any experience and evidence for and against the use of 95% or ~70% EtOH for longterm invertebrate (specifically gorgonian coral) specimen preservation for collections. More and more institutions seem to be turning to 95% EtOH, however, there are numerous conflicting thoughts and evidence with regards the use of higher concentrations for longterm specimen storage. Furthermore, I understand that there are 2 types of high EtOH concentration; clean and dirty with the latter being far cheaper but unsuitable for genetic analyses.

Whilst collections are indeed a taxonomic concern, this does include the use of molecular work as part of the suite of tools in determining species differences. The collections I have worked in using ~95% EtOH have had good success with both storage and genetic work, but these specimens may often be a maximum of 1 - 2 decades old. Conversely, others mention caveats such as the high concentration dehydrating the specimen to such an extent that it disrupts the integrity of both specimen and DNA. At the same time the often high water content of specimens is said to bring down the EtOH concentration which may also cause issues with specimen degradation.

Please excuse the somewhat rudimentary nature of my question, there does seem a tremendous amount of confliction in what is recommend and therefore it would be really valuable to hear what people have to say and recommend from the list.

Thank you for your time with this matter and look forward to hearing any responses

Sonia

--
Sonia J. Rowley PhD
Research Affiliate
Bernice Pauahi Bishop Museum
1525 Bernice St, Honolulu,
HI 96817,,USA
+1 808 348 6224

Research Affiliate
University of Hawai'i at Manoa
2538 McCarthy Mall, Edmondson 312
Honolulu, HI 96822
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20140729/474ff609/attachment-0001.html 


More information about the Nhcoll-l mailing list