[Nhcoll-l] dna storage tubes for -80°C

[Dirk Neumann]

Hi all,

I would like to know if there are any specific experiences (good _and_ 
bad) on DNA-storage tubes (normal freezing as we don't have liquid-N 
storage).

We are using Matrix racks (sepra seals) and I want to change to high 
quality skrew-lid tubes, but I am sceptical with the silicone gasket in 
combination with the internal thread of the tubes.

Thermo says that the tubes are tight sealing (from liquid-N to normal 
freezing and even room temperature), but if the gasket starts to 
crumble, then the tubes necks are open. Also, gaskets are made of 
silicone, which might cause issues with cold-flowing behaviour and life 
span of the material.

Curious to hear your thoughts, especially if you have experiences where 
things turned out to be not that good as originally promised by the 
manufacturer (also off-line, if you wouldn't like to post any failures 
to the broad public)


Thanks in advance for any helpful feedback

Dirk


-- 
Dirk Neumann

Tel: 089 / 8107-111
Fax: 089 / 8107-300
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Staatliche Naturwissenschaftliche Sammlungen Bayerns
Zoologische Staatssammlung München
Dirk Neumann, Sektion Ichthyologie / DNA-Labor
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---------

Dirk Neumann

Tel: +49-89-8107-111
Fax: +49-89-8107-300
email: Dirk.Neumann(a)zsm.mwn.de

postal address:

Bavarian Natural History Collections
The Bavarian State Collection of Zoology
Dirk Neumann, Section Ichthyology / DNA-Lab
Muenchhausenstr. 21
81247 Munich (Germany)

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