[Nhcoll-l] FW: [CRUST-L:9947] Mercuric chloride and sequencing

[Joachim Haendel]

Hello Kareen & Rachael,
and hello Dirk,

well - this is really interesting. But I can't say anything about this. To my knowledge there are no researches 
on this topic. Mercury(II) chloride is an effective antiseptic and disinfectant and it is corrosive. It is thinkable 
that it will damage the DNA. Maybe someone could try it out?! I think many museo-biologists want to know 
the results.

Greetings
Joachim



---- On 24 Jan 2017 at 8:36, Dirk Neumann wrote:

> Good morning Kareen & Rachael,
> 
> I am not sure if there is first hand experience how mercuric chloride 
> behaves exactly, but it was used as fixative especially for marine 
> invertebrates in the early 20th century (sublimate; cf. Piechocki & 
> Händel, 2007. Makroskopische Präparationstechnik, Wirbellose, 5th 
> Edition) and as early antibiotic (e.g. against syphilis) because of its 
> toxic properties. So I would assume that it does interact with tissues 
> and DNAs and consequently do influence sequencing results.
> 
> Perhaps Joachim can add more details ?
> 
> Hope this helps
> Dirk
> 
> Am 23.01.2017 um 23:54 schrieb Kareen Schnabel:
> >
> > Good morning all, I thought this listserv may have some valuable input 
> > into my colleague´s query to the Crustacean listserv. Please see query 
> > below and please reply to Rachael Peart rachaelapeart at gmail.com 
> > <mailto:rachaelapeart at gmail.com>.**
> >
> > Thanks
> >
> > Kareen
> >
> >
> > DrKareen Schnabel
> > Marine Biologist
> >
> > *From:*crust-l-request at vims.edu [mailto:crust-l-request at vims.edu] *On 
> > Behalf Of *Rachael Peart
> > *Sent:* Tuesday, 24 January 2017 11:49 a.m.
> > *To:* crust-l at vims.edu
> > *Subject:* [CRUST-L:9947] Mercuric chloride and sequencing
> >
> > Hi all,
> >
> > I have come across some amphipod samples that were captured in traps 
> > using mercuric chloride.
> >
> > I am curious to know if this chemical can affect molecular sequencing 
> > - ie COI or 18S?
> >
> > Does it completely destroy the amounts, or do you get traces, or no 
> > damage?
> >
> > Thanks in advance
> >
> > Rachael Peart
> >
> >



--  
Joachim Haendel                                                          
Center of Natural Sciences Collections
of the Martin-Luther-University
- Entomological Collection  -                         

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Germany    

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Email: joachim.haendel at zns.uni-halle.de