[Nhcoll-l] FW: [CRUST-L:9947] Mercuric chloride and sequencing

[Rachael Peart]

Thanks for the discussion,
It looks like due to it's toxic nature, there is no extraction possible
from animals preserved with mercuric chloride based products.
Found these articles.
O'Leary et al, 1994 - "The importance of fixation procedures on DNA
template and its suitability for solution-phase polymerase chain reaction
and PCR *in situ *hybridisation."
The Histochemical Journal 26 (4): 337 - 346.
C. Schander and K.M. Halanych, 2003. "DNA, PCR and formalinized animal
tissue - a short review and protocols." Organisms Diversity and Evolution 3
(3): 195 - 205.

Thanks anyway

Rachael

On Tue, Jan 24, 2017 at 9:50 PM, Joachim Haendel <
joachim.haendel at zns.uni-halle.de> wrote:

> Hello Kareen & Rachael,
> and hello Dirk,
>
> well - this is really interesting. But I can't say anything about this. To
> my knowledge there are no researches
> on this topic. Mercury(II) chloride is an effective antiseptic and
> disinfectant and it is corrosive. It is thinkable
> that it will damage the DNA. Maybe someone could try it out?! I think many
> museo-biologists want to know
> the results.
>
> Greetings
> Joachim
>
>
>
> ---- On 24 Jan 2017 at 8:36, Dirk Neumann wrote:
>
> > Good morning Kareen & Rachael,
> >
> > I am not sure if there is first hand experience how mercuric chloride
> > behaves exactly, but it was used as fixative especially for marine
> > invertebrates in the early 20th century (sublimate; cf. Piechocki &
> > Händel, 2007. Makroskopische Präparationstechnik, Wirbellose, 5th
> > Edition) and as early antibiotic (e.g. against syphilis) because of its
> > toxic properties. So I would assume that it does interact with tissues
> > and DNAs and consequently do influence sequencing results.
> >
> > Perhaps Joachim can add more details ?
> >
> > Hope this helps
> > Dirk
> >
> > Am 23.01.2017 um 23:54 schrieb Kareen Schnabel:
> > >
> > > Good morning all, I thought this listserv may have some valuable input
> > > into my colleague´s query to the Crustacean listserv. Please see query
> > > below and please reply to Rachael Peart rachaelapeart at gmail.com
> > > <mailto:rachaelapeart at gmail.com>.**
> > >
> > > Thanks
> > >
> > > Kareen
> > >
> > >
> > > DrKareen Schnabel
> > > Marine Biologist
> > >
> > > *From:*crust-l-request at vims.edu [mailto:crust-l-request at vims.edu] *On
> > > Behalf Of *Rachael Peart
> > > *Sent:* Tuesday, 24 January 2017 11:49 a.m.
> > > *To:* crust-l at vims.edu
> > > *Subject:* [CRUST-L:9947] Mercuric chloride and sequencing
> > >
> > > Hi all,
> > >
> > > I have come across some amphipod samples that were captured in traps
> > > using mercuric chloride.
> > >
> > > I am curious to know if this chemical can affect molecular sequencing
> > > - ie COI or 18S?
> > >
> > > Does it completely destroy the amounts, or do you get traces, or no
> > > damage?
> > >
> > > Thanks in advance
> > >
> > > Rachael Peart
> > >
> > >
>
>
>
> --
> Joachim Haendel
> Center of Natural Sciences Collections
> of the Martin-Luther-University
> - Entomological Collection  -
>
> Domplatz 4
> D-06099 Halle (Saale)
> Germany
>
> Phone:  +49 345 - 55 26 447
> Fax: +49 345 - 55 27 248
> Email: joachim.haendel at zns.uni-halle.de
>
>
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20170125/f3d108df/attachment.html