[Nhcoll-l] Formaldehyde and bone preservation

Fri May 26 14:53:12 EDT 2017

*Just so no one is concerned, *we have a protocol in place that has
received BioSafety clearance to perform the macerations in an appropriate
facility without formalin fixation. We were just considering formalin
fixation as an alternative.

On Fri, May 26, 2017 at 11:49 AM, David Katz <dckatz at ucdavis.edu> wrote:

> Thanks everyone. We will stick with out original (non-formalin) plan. The
> reason for considering formalin is because these animals are potentially
> disease vectors. By "short" I meant fixation for a sufficient length of
> time that the tissue would be fixed. I was contrasting it with a situation
> where you might have an animal sitting in formalin for years (sometimes
> happens in anatomy classes for dissection specimens that don't get used).
>
> Really appreciate the help.
>
> David
>
> On Fri, May 26, 2017 at 1:59 AM, Dirk Neumann <dirk.neumann at zsm.mwn.de>
> wrote:
>
>> Dear David,
>>
>> because of the low pH formaldehyde decalcifies the the bones and can make
>> (tiny) bones brittle. Also, because of the covalent bonding inside tissues,
>> enzymatic degradation of tissues is hindered. Unbound formalin escaping
>> during maceration from specimens could cause formaldehyde gas to get
>> trapped under the lid of the maceration tank and might cause health issues
>> when opening the tank.
>>
>> I am also not sure if "short" fixation would result in sufficient
>> penetration into tissues, so you would need to expose the bodied for
>> sufficient long time (depending on the size and amount of tissue)
>>
>> This would be the immediate concerns that jump into my mind, but there
>> might be more.
>>
>> Hope this helps
>> Dirk
>>
>>
>>
>> Am 26.05.2017 um 03:45 schrieb David Katz:
>>
>> Hi everyone,
>>
>> We will have a number of medium bodied primate carcasses that we plan to
>> macerate in order to preserve the skeletons. We've been told that fixing
>> with formaldehyde prior to maceration can negatively affect the bones.
>>
>> What are the consequences? Is this an issue for both short and long
>> duration fixation, or just long? Thanks very much.
>>
>> David
>>
>> --
>> David C. Katz, Ph.D.
>> Postdoctoral Fellow
>> Benedikt Hallgrimsson Lab
>> University of Calgary
>>
>> Research Associate
>> Department of Anthropology
>> University of California, Davis
>>
>> ResearchGate profile
>> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.researchgate.net_profile_David-5FKatz29&d=DwMFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=l2m1TxetuJFFU3F1X1583Si31tc_rwjKAPbjbBGfOiA&s=SKyKxGfcWWp-HTvfqIULUy2uF4nVwFZfmMCrJEbpoyI&e=>
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>> --
>> Dirk Neumann
>>
>> Tel: 089 / 8107-111
>> Fax: 089 / 8107-300
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>>
>> Postanschrift:
>>
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>> Dirk Neumann, Sektion Ichthyologie / DNA-Storage
>> Münchhausenstr. 21
>> 81247 München
>>
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>>
>> ---------
>>
>> Dirk Neumann
>>
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>> Fax: +49-89-8107-300 <+49%2089%208107300>
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>>
>> postal address:
>>
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>>
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>> natural history collections to ensure their continuing value to
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>
>
> --
> David C. Katz, Ph.D.
> Postdoctoral Fellow
> Benedikt Hallgrimsson Lab
> University of Calgary
>
> Research Associate
> Department of Anthropology
> University of California, Davis
>
> ResearchGate profile <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.researchgate.net_profile_David-5FKatz29&d=DwIFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=AL11lOuKOnBhGSeTUVFuGk2y9BlA74Lalfghfyf-vKk&s=GCvWt0HuiAfJ1wQL_8ZG9-0r_z7XUdfM4KjPWcTPMaY&e= >
> Personal webpage
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__davidckatz.wordpress.com_&d=DwIFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=AL11lOuKOnBhGSeTUVFuGk2y9BlA74Lalfghfyf-vKk&s=9a8B7reigQPmPRbbOBfG6H3_QZEKK1ObJfpuFHTsAVE&e= >
>

-- 
David C. Katz, Ph.D.
Postdoctoral Fellow
Benedikt Hallgrimsson Lab
University of Calgary

Research Associate
Department of Anthropology
University of California, Davis

ResearchGate profile <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.researchgate.net_profile_David-5FKatz29&d=DwIFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=AL11lOuKOnBhGSeTUVFuGk2y9BlA74Lalfghfyf-vKk&s=GCvWt0HuiAfJ1wQL_8ZG9-0r_z7XUdfM4KjPWcTPMaY&e= >
Personal webpage
<https://urldefense.proofpoint.com/v2/url?u=https-3A__davidckatz.wordpress.com_&d=DwIFaQ&c=-dg2m7zWuuDZ0MUcV7Sdqw&r=CLFZJ3fvGSmDp7xK1dNZfh6uGV_h-8NVlo3fXNoRNzI&m=AL11lOuKOnBhGSeTUVFuGk2y9BlA74Lalfghfyf-vKk&s=9a8B7reigQPmPRbbOBfG6H3_QZEKK1ObJfpuFHTsAVE&e= >
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