[Nhcoll-l] comparative quality cryogenic vials

Wed Aug 19 17:19:33 EDT 2020

John

We have been using these Nalgene tubes in our collection and for collecting in all environments for over 15 years and have had no issues with storage in 95% ethanol, RNALater or liquid nitrogen environments:  https://www.fishersci.com/shop/products/nalgene-general-long-term-storage-cryogenic-tubes/033377d

I agree with Dirk that whatever make you go with, externally threaded tubes with no O-ring gasket are the way to go.

Andy

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Andy Bentley
Ichthyology Collection Manager
University of Kansas
Biodiversity Institute
Dyche Hall
1345 Jayhawk Boulevard
Lawrence, KS, 66045-7561
USA

Tel: (785) 864-3863
Fax: (785) 864-5335
Email: abentley at ku.edu<mailto:abentley at ku.edu>
http://ichthyology.biodiversity.ku.edu<http://ichthyology.biodiversity.ku.edu/>

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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> on behalf of Dirk Neumann <neumann at snsb.de>
Reply-To: "neumann at snsb.de" <neumann at snsb.de>
Date: Wednesday, August 19, 2020 at 4:05 PM
To: "nhcoll-l at mailman.yale.edu" <nhcoll-l at mailman.yale.edu>
Subject: Re: [Nhcoll-l] comparative quality cryogenic vials

Dear John,

I would prefer NUNC Store-it tubes (article 264300; https://www.thermofisher.com/order/catalog/product/264262#/264262<https://nam10.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.thermofisher.com%2Forder%2Fcatalog%2Fproduct%2F264262%23%2F264262&data=02%7C01%7Cabentley%40ku.edu%7Cdf385ddaaca24707b7f808d844839aa4%7C3c176536afe643f5b96636feabbe3c1a%7C0%7C0%7C637334679345844493&sdata=2fl7vyz6UNbMvx1fHGiytMNCT9cN3ggCZ%2BkbqTVaI1I%3D&reserved=0>)

We tested them against Sarsted and other brands in a warm water bath > 50°C and they were among the tightest ones; also, they DON'T have an silicone O-ring (which will fail after some time); we had to replace poor quality tubes twice so far and besides necessary replacement costs and time needed to transfer tissues the huge evaporation loss (> 50% in some tubes) surely affected the integrity of the tissues / DNA.

The NUNC-tubes have a flange and really need to be screwed down tight (which is a bit annoying), but we have them on storage since 2004, use them in the tropic in the field and their performance is excellent. Bit more expensive, but this slightly higher investment pays off in the long run.

Hope this helps,
with best wishes
Dirk

Am 17.08.2020 um 23:42 schrieb John Edwin Lattke Bravo:
Hello,

Does anybody have any thoughts or experience to share regarding the comparative merits of 2ml cryogenic vials by Corning, Sarstedt and Granier for long term storage of insects in ethanol? Is the quality amongst the three similar? Positive experiences? Negative one? Just needing some user experience to help decide what might deliever the best bang for the buck (or real, in my case).

Obrigado,

John Lattke

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