The biggest issue with diceCT has been soft-tissue shrinkage, but this has been remedied by using Sorensen's buffer as published by Dawood et al. 2021. This is the buffer used in the Gray protocol shared by Andy. Links to the Dawood paper as well as others of potential interest are below.
Dawood et al. 2021: https://www.nature.com/articles/s41598-021-99202-2
Early et al. 2020: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0238783
Hedrick et al. 2018: https://www.researchgate.net/publication/325862458_Assessing_Soft-Tissue_Shrinkage_Estimates_in_Museum_Specimens_Imaged_With_Diffusible_Iodine-Based_Contrast-Enhanced_Computed_Tomography_diceCT
Best,
Zach
--
Zachary S. Randall
Digital Imaging Manager
Florida Museum of Natural History
1659 Museum Road, Gainesville, FL 32611-7800
(352) 273-1958 | zrandall at flmnh.ufl.edu<mailto:zrandall at flmnh.ufl.edu>
https://zachrandall.weebly.com/
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https://sketchfab.com/FloridaMuseum
From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> On Behalf Of Bentley, Andrew Charles
Sent: Wednesday, March 23, 2022 10:01 AM
To: Esther Dondorp <esther.dondorp at naturalis.nl>; nhcoll-l at mailman.yale.edu
Subject: Re: [Nhcoll-l] DICE-CT on NHspecimens
[External Email]
Esther
The oVert project here in the US has had extensive experience with diceCT and have developed the attached protocol. Jaimi Gray would be the person to contact in this regard - jaimigray at floridamuseum.ufl.edu. She also co-authored the following publication - https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467<https://urldefense.proofpoint.com/v2/url?u=https-3A__onlinelibrary.wiley.com_doi_full_10.1002_ece3.7467&d=DwMFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=BsjUEaAzErVnOJA4kXSO_g&m=wxRvIT13YXc8ucW2LYysYhhystArFtc5AC6Huxs4v4j4YjmlTq3sRNo8vY0lOvOL&s=PCoqPP0RplincQmDRfDfKbAf08gmtmu0zMnevmF7kYE&e=>
Hope that helps
Andy
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Andy Bentley
Ichthyology Collection Manager
University of Kansas
Biodiversity Institute
Dyche Hall
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Tel: (785) 864-3863<tel:%28785%29%20864-3863>
Fax: (785) 864-5335<tel:%28785%29%20864-5335>
Email: abentley at ku.edu<mailto:abentley at ku.edu>
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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> On Behalf Of Esther Dondorp
Sent: Wednesday, March 23, 2022 4:24 AM
To: nhcoll-l at mailman.yale.edu
Subject: [Nhcoll-l] DICE-CT on NHspecimens
Dear colleagues,
Does anyone have experience with Diffusible Iodine-based Contrast-enhanced Computed Tomography (DICE-CT) performed on fluid preserved natural history specimens? I want to know if it is really non destructive, if the specimens do stay stable for the next many years or more after this treatment?
Many thanks!
Esther Dondorp
Senior Collectiebeheerder
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