[Nhcoll-l] DICE-CT on NHspecimens

[Milensky, Christopher]

I agree with everything Greg stated and we consider it destructive sampling as well.  The staining and other alterations are permanent and specimens should be stored separately when returned to the collection with notes about the staining and scanning methods used.  In the birds we tested, the internal organs are stained permanently as shown in scans done before and after 'destaining'.

Chris


Christopher Milensky
Collection Manager
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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> On Behalf Of Watkins-Colwell, Gregory
Sent: Wednesday, March 23, 2022 9:59 AM
To: Esther Dondorp <esther.dondorp at naturalis.nl>; nhcoll-l at mailman.yale.edu
Subject: Re: [Nhcoll-l] DICE-CT on NHspecimens

External Email - Exercise Caution
We consider it "destructive sampling" because the specimen is forever altered.  Rinsing in multiple washes of ethanol can get the specimen back to near original color (though likely pulling out more pigments and other etoh-soluble organics in the process).  However, the odds are pretty good that there's still iodine in the specimen somewhere... and that could impact future use.  So because of that we consider it a form of destructive sampling.

I also suspect there is damage done during the long soak in the aqueous solution.  I know some folks have used iodine dissolved in ethanol because of that reason and it may help, though it also might not stain as well thus permanently altering a specimen for insufficient gain.  I tend to just document the process, call it destructive, and not dictate that the iodine be in ETOH.  I also request that it be returned in ETOH, but not "washed".  I can do that here and have more control over how many ETOH soaks it gets before going back into the collection.  Generally they all get their own jar too so they can't accidentally stain a room-mate.  And I'd rather there be slightly more iodine left in the specimen than risk damaging it by repeated ETOH changes.

My 2 cents.... Maybe 3.

Greg




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Gregory J. Watkins-Colwell
Sr. Collection Manager, Herpetology and Ichthyology
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From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu<mailto:nhcoll-l-bounces at mailman.yale.edu>> On Behalf Of Esther Dondorp
Sent: Wednesday, March 23, 2022 5:24 AM
To: nhcoll-l at mailman.yale.edu<mailto:nhcoll-l at mailman.yale.edu>
Subject: [Nhcoll-l] DICE-CT on NHspecimens

Dear colleagues,

Does anyone have experience with Diffusible Iodine-based Contrast-enhanced Computed Tomography (DICE-CT) performed on fluid preserved natural history specimens? I want to know if it is really non destructive, if the specimens do stay stable for the next many years or more after this treatment?

Many thanks!

Esther Dondorp
Senior Collectiebeheerder
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