[Nhcoll-l] DICE-CT on NHspecimens

Wed Mar 23 15:40:23 EDT 2022

Simon et al.,
I know from experience with histology that certain mordants such as
phosphotungstic and phosphomolybdic acid enhance staining of paraffin
sections. I assumed that the iodine might be doing something similar, but
I'm just guessing.

Dave

On Wed, Mar 23, 2022 at 2:22 PM Simon Moore <couteaufin at btinternet.com>
wrote:

> Many thanks for these ‘aside’ observations Dave,
>
> I know nothing of the DICE-CT technique having never needed to use it;
> however, the thought that Lugol’s iodine or the use of iodine as a
> pre-staining mordant for alizarin transparency preparations is most
> interesting. Do you or does anyone have any idea of how this enhancement
> might work?
>
> With all good wishes, Simon
>
> Simon Moore MIScT, RSci, FLS, ACR
> Conservator of Natural Sciences and Cutlery Historian,
>
> www.natural-history-conservation.com
>
>
>
>
> On 23 Mar 2022, at 18:49, David Cannatella <catfish at utexas.edu> wrote:
>
> Hi all,
> I thought I'd share some non-experimental observations on Lugol's solution
> and its effect on formalin fixed material, specifically frogs.
>
> I have not used in DICE-CT. However, I have dissected muscles on hundreds
> of frogs, using Lugol's to improve visualization of muscles and enhance
> photography.
>
> I used a "standard" histology-type solution of iodine and potassium iodide
> in 70% ethanol, not water. I'd reflect the skin to allow the stain to
> penetrate quickly, and did the dissections in layers, exposing
> deeper muscles and re-staining. I moved the specimen directly for storage
> alcohol to the Lugols with no rinsing. Often a specimen was re-stained
> several times, and destained in 70% ethanol (which was discarded after
> a few uses). I never noticed any desiccation or softening of the muscles or
> other organs such as the liver or intestines, but I was not collecting data
> on this.
> I did not measure changes in pH, but I threw out the Lugols after a week
> to avoid potential acidification. I did not notice softening of bones, as
> some have seen.
>
> After muscle dissections, I would sometimes clear and stain the specimen
> using standard alizarin-alcian blue techniques. The specimens seemed to
> take up both the alizarin and alcian stains more quickly and evenly
> than did an untreated specimen.  Perhaps this was a mordant effect of the
> iodine.
>
> Based on these qualitative but numerous observations, as a curator I would
> have little reservation about allowing DICE-CT staining of specimens, but
> bear in mind that I was working with generally smaller vertebrates that
> were sufficiently stained in 3-6 hours.
>
> Of course, it should be noted in the catalog that the specimen was treated
> in this way, to inform future researchers.
>
> Best,
> Dave
>
>
>
>
>
>
>
>
> On Wed, Mar 23, 2022 at 9:01 AM Bentley, Andrew Charles <abentley at ku.edu>
> wrote:
> Esther
>
>
>
> The oVert project here in the US has had extensive experience with diceCT
> and have developed the attached protocol.  Jaimi Gray would be the person
> to contact in this regard - jaimigray at floridamuseum.ufl.edu.  She also
> co-authored the following publication -
> https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.7467
>
>
>
> Hope that helps
>
>
>
> Andy
>
>     A  :             A  :             A  :
>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>     V                V                V
> Andy Bentley
> Ichthyology Collection Manager
> University of Kansas
> Biodiversity Institute
>
> Dyche Hall
> 1345 Jayhawk Boulevard
> Lawrence, KS, 66045-7561
> USA
>
> Tel: (785) 864-3863
> Fax: (785) 864-5335
> Email: abentley at ku.edu
>
> ORCID: https://orcid.org/0000-0002-3093-1258
>
> http://ichthyology.biodiversity.ku.edu
>
>     A  :             A  :             A  :
>  }<(((_°>.,.,.,.}<(((_°>.,.,.,.}<)))_°>
>     V                V                V
>
>
>
> From: Nhcoll-l <nhcoll-l-bounces at mailman.yale.edu> On Behalf Of Esther
> Dondorp
> Sent: Wednesday, March 23, 2022 4:24 AM
> To: nhcoll-l at mailman.yale.edu
> Subject: [Nhcoll-l] DICE-CT on NHspecimens
>
>
>
> Dear colleagues,
>
>
>
> Does anyone have experience with Diffusible Iodine-based Contrast-enhanced
> Computed Tomography (DICE-CT) performed on fluid preserved natural history
> specimens? I want to know if it is really non destructive, if the specimens
> do stay stable for the next many years or more after this treatment?
>
>
>
> Many thanks!
>
>
>
> Esther Dondorp
>
> Senior Collectiebeheerder
>
>
>
>
>
>
>
> - -
> esther.dondorp at naturalis.nl - www.naturalis.nl
> Darwinweg 2, 2333 CR Leiden
> Postbus 9517, 2300 RA Leiden
>
>
>
>
> _______________________________________________
> Nhcoll-l mailing list
> Nhcoll-l at mailman.yale.edu
> https://mailman.yale.edu/mailman/listinfo/nhcoll-l
>
> _______________________________________________
> NHCOLL-L is brought to you by the Society for the Preservation of
> Natural History Collections (SPNHC), an international society whose
> mission is to improve the preservation, conservation and management of
> natural history collections to ensure their continuing value to
> society. See http://www.spnhc.org for membership information.
> Advertising on NH-COLL-L is inappropriate.
> >> This message is from an external sender. Learn more about why this <<
> >> matters at https://links.utexas.edu/rtyclf.                        <<
>
>
> --
> David Cannatella
> Assoc. Director, Biodiversity Center
> Department of Integrative Biology
> Patterson Labs, 2415 Speedway
> University of Texas, Austin, Texas 78712
> www.cannatellalab.org
> 512.453.1620 cell
> _______________________________________________
> Nhcoll-l mailing list
> Nhcoll-l at mailman.yale.edu
> https://mailman.yale.edu/mailman/listinfo/nhcoll-l
>
> _______________________________________________
> NHCOLL-L is brought to you by the Society for the Preservation of
> Natural History Collections (SPNHC), an international society whose
> mission is to improve the preservation, conservation and management of
> natural history collections to ensure their continuing value to
> society. See http://www.spnhc.org for membership information.
> Advertising on NH-COLL-L is inappropriate.
>
>
>

-- 
David Cannatella
Assoc. Director, Biodiversity Center
Department of Integrative Biology
Patterson Labs, 2415 Speedway
University of Texas, Austin, Texas 78712
www.cannatellalab.org
512.453.1620 cell
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20220323/a0af8765/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: PastedGraphic-2.tiff
Type: image/tiff
Size: 38900 bytes
Desc: not available
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20220323/a0af8765/attachment-0001.tiff>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: MA logo.jpg
Type: image/jpeg
Size: 19375 bytes
Desc: not available
URL: <http://mailman.yale.edu/pipermail/nhcoll-l/attachments/20220323/a0af8765/attachment-0001.jpg>